| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
*
Laboratoire d’Immunologie Moléculaire, Université de Bordeaux II, Bordeaux, France;
Unité d’Immunogénétique Cellulaire, Institut Pasteur, Paris, France; and
Shanghai Institute of Biochemistry, Shanghai, China
IL-2 induces growth, differentiation, and/or apoptosis of lymphoid
cells. To study further the molecular basis of IL-2 function, we used a
cDNA subtraction approach involving a cell line grown in IL-2 or IL-4.
From the corresponding library, 66 nonredundant sequences were
characterized; 16 of them encode identified proteins. The kinetics of
in vitro expression of 8 selected sequences, the functions of which
could be associated with IL-2-induced T cell
activation/differentiation, was investigated using an IL-2-dependent T
cell line. IL-2 increased the expression of cytoskeleton proteins
(
-tubulin), oncogene-regulating proteins (CCCTC-binding factor, Jun
inhibitor factor-1), and transcription factors (E2F-4, cyclic
AMP-responsive element-binding protein, zhx-1). IL-2 also regulated the
expression of genes coding for multifunctional proteins, e.g.,
ß-catenin and nucleolin. These results were verified using Con
A-induced T cell blasts stimulated or not by IL-2. The in vivo
expression of four of these genes was also analyzed in spleen and lymph
node cells of IL-2-deficient and MRL/lpr mice, which
both have high numbers of activated cells, but the latter have intact
IL-2 expression. The expression of ß-catenin, CCCTC-binding factor,
Jun inhibitor factor-1, and nucleolin was significantly higher in
MRL/lpr animals. A similar analysis of thymocytes from
IL-2-/- and IL-2+/- mice demonstrated the
same expression patterns of the 4 sequences in these strains. The
expression of the IL-2-induced genes described herein is similar to the
regulatory pattern of IL-2R. Taken together, our data provide
additional evidence for the pleiotropic action of IL-2 in the periphery
and IL-2 independence of molecular processes involved in thymocyte
differentiation.
This article has been cited by other articles:
|
|
 |
|
  F. Gesbert, J.-L. Moreau, and J. Theze IL-2 Responsiveness of CD4 and CD8 lymphocytes: further investigations with human IL-2R{beta} transgenic mice Int. Immunol., August 1, 2005; 17(8): 1093 - 1102. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. E. Kovanen, A. Rosenwald, J. Fu, E. M. Hurt, L. T. Lam, J. M. Giltnane, G. Wright, L. M. Staudt, and W. J. Leonard Analysis of gamma c-Family Cytokine Target Genes. IDENTIFICATION OF DUAL-SPECIFICITY PHOSPHATASE 5 (DUSP5) AS A REGULATOR OF MITOGEN-ACTIVATED PROTEIN KINASE ACTIVITY IN INTERLEUKIN-2 SIGNALING J. Biol. Chem., February 7, 2003; 278(7): 5205 - 5213. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Grinstein, P. Wernet, P. J.F. Snijders, F. Rosl, I. Weinert, W. Jia, R. Kraft, C. Schewe, M. Schwabe, S. Hauptmann, et al. Nucleolin as Activator of Human Papillomavirus Type 18 Oncogene Transcription in Cervical Cancer J. Exp. Med., October 21, 2002; 196(8): 1067 - 1078. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Chastagner, J. Reddy, and J. Theze Lymphoadenopathy in IL-2-Deficient Mice: Further Characterization and Overexpression of the Antiapoptotic Molecule Cellular FLIP J. Immunol., October 1, 2002; 169(7): 3644 - 3651. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Y. Hsia, S. Cheng, A. M. Owyang, S. F. Dowdy, and H.-C. Liou c-Rel regulation of the cell cycle in primary mouse B lymphocytes Int. Immunol., August 1, 2002; 14(8): 905 - 916. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. C. S. Bizario, F. A. Castro, J. F. Sousa, R. N. Fernandes, A. D. Damiao, M. K. Oliveira, P. V. B. Palma, R. E. Larson, J. C. Voltarelli, and E. M. Espreafico Myosin-V colocalizes with MHC class II in blood mononuclear cells and is up-regulated by T-lymphocyte activation J. Leukoc. Biol., February 1, 2002; 71(2): 195 - 204. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Reddy, P. Chastagner, L. Fiette, X. Liu, and J. Theze IL-2-induced tumor necrosis factor (TNF)-{beta} expression: further analysis in the IL-2 knockout model, and comparison with TNF-{{alpha}}, lymphotoxin-{beta}, TNFR1 and TNFR2 modulation Int. Immunol., February 1, 2001; 13(2): 135 - 147. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Eckenberg, J.-L. Moreau, O. Melnyk, and J. Theze IL-2R{beta} Agonist P1-30 Acts in Synergy with IL-2, IL-4, IL-9, and IL-15: Biological and Molecular Effects J. Immunol., October 15, 2000; 165(8): 4312 - 4318. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. J. Greasley, C. Bonnard, and B. Amati Myc induces the nucleolin and BN51 genes: possible implications in ribosome biogenesis Nucleic Acids Res., January 15, 2000; 28(2): 446 - 453. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. J. Westmark and J. S. Malter Up-regulation of Nucleolin mRNA and Protein in Peripheral Blood Mononuclear Cells by Extracellular-regulated Kinase J. Biol. Chem., January 5, 2001; 276(2): 1119 - 1126. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
