| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
*
Immune Cell Biology Program, Naval Medical Research Institute, Bethesda, MD 20889;
The Henry M. Jackson Foundation for the Advancement of Military Medicine, U.S. Military HIV Research Program, Bethesda, MD 20889;
Laboratory of Immunoregulation, National Institute of Allergy and Infectious Disease, National Institutes of Health, Bethesda, MD 20892; and
§
Department of Internal Medicine, Uniformed Services University of the Health Sciences, Bethesda, MD 20889
Intracellular signals that mediate differentiation of pluripotent
hemopoietic progenitors to dendritic cells (DC) are largely undefined.
We have previously shown that protein kinase C (PKC) activation (with
phorbol ester (PMA) alone) specifically induces differentiation of
primary human CD34+ hemopoietic progenitor cells (HPC) to
mature DC. We now find that cytokine-driven (granulocyte-macrophage CSF
and TNF-
) CD34+ HPC
DC differentiation is
preferentially blocked by inhibitors of PKC activation. To further
identify intracellular signals and downstream events important in
CD34+ HPCDC differentiation we have characterized a
human leukemic cell line model of this process. The CD34+
myelomonocytic cell line KG1 differentiates into dendritic-like cells
in response to granulocyte-macrophage CSF plus TNF-, or PMA (with or
without the calcium ionophore ionomycin, or TNF-), with different
stimuli mediating different aspects of the process. Phenotypic DC
characteristics of KG1 dendritic-like cells include morphology (loosely
adherent cells with long neurite processes), MHC I+/MHC
IIbright/CD83+/CD86+/CD14-
surface Ag expression, and RelB and DC-CK1 gene expression. Functional
DC characteristics include fluid phase macromolecule uptake
(FITC-dextran) and activation of resting T cells. Comparison of KG1 to
the PMA-unresponsive subline KG1a reveals differences in expression of
TNF receptors 1 and 2; PKC isoforms , ßI, ßII, and µ; and
RelB, suggesting that these components/pathways are important for DC
differentiation. Together, these findings demonstrate that cytokine or
phorbol ester stimulation of KG1 is a model of human CD34+
HPC to DC differentiation and suggest that specific intracellular
signaling pathways mediate specific events in DC lineage
commitment.
This article has been cited by other articles:
|
|
 |
|
  S. J. A. M. Santegoets, A. J. M. van den Eertwegh, A. A. van de Loosdrecht, R. J. Scheper, and T. D. de Gruijl Human dendritic cell line models for DC differentiation and clinical DC vaccination studies J. Leukoc. Biol., December 1, 2008; 84(6): 1364 - 1373. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. L. Herrmann, R. S. Agrawal, S. F. Connolly, R. L. McCaffrey, J. Schlomann, and D. J. Kusner MHC Class II levels and intracellular localization in human dendritic cells are regulated by calmodulin kinase II J. Leukoc. Biol., September 1, 2007; 82(3): 686 - 699. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Majewski, T. O. Bose, F. C. M. Sille, A. M. Pollington, E. Fiebiger, and M. Boes Protein kinase C delta stimulates antigen presentation by Class II MHC in murine dendritic cells Int. Immunol., June 1, 2007; 19(6): 719 - 732. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. J. Bahlis, A. M. King, D. Kolonias, L. M. Carlson, H. Y. Liu, M. A. Hussein, H. R. Terebelo, G. E. Byrne Jr, B. L. Levine, L. H. Boise, et al. CD28-mediated regulation of multiple myeloma cell proliferation and survival Blood, June 1, 2007; 109(11): 5002 - 5010. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Roychowdhury, P. M. Vyas, and C. K. Svensson Formation and Uptake of Arylhydroxylamine-Haptenated Proteins in Human Dendritic Cells Drug Metab. Dispos., April 1, 2007; 35(4): 676 - 681. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Bachmann, C. Dragoi, M. A. Poleganov, J. Pfeilschifter, and H. Muhl Interleukin-18 directly activates T-bet expression and function via p38 mitogen-activated protein kinase and nuclear factor-{kappa}B in acute myeloid leukemia-derived predendritic KG-1 cells Mol. Cancer Ther., February 1, 2007; 6(2): 723 - 731. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. A. Obeng, L. M. Carlson, D. M. Gutman, W. J. Harrington Jr, K. P. Lee, and L. H. Boise Proteasome inhibitors induce a terminal unfolded protein response in multiple myeloma cells Blood, June 15, 2006; 107(12): 4907 - 4916. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. L. Herrmann, C. T. Morita, K. Lee, and D. J. Kusner Calmodulin kinase II regulates the maturation and antigen presentation of human dendritic cells J. Leukoc. Biol., December 1, 2005; 78(6): 1397 - 1407. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. A. Ryan, G. F. Gerberick, L. A. Gildea, B. C. Hulette, C. J. Betts, M. Cumberbatch, R. J. Dearman, and I. Kimber Interactions of Contact Allergens with Dendritic Cells: Opportunities and Challenges for the Development of Novel Approaches to Hazard Assessment Toxicol. Sci., November 1, 2005; 88(1): 4 - 11. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. J. Cejas, L. M. Carlson, D. Kolonias, J. Zhang, I. Lindner, D. D. Billadeau, L. H. Boise, and K. P. Lee Regulation of RelB Expression during the Initiation of Dendritic Cell Differentiation Mol. Cell. Biol., September 1, 2005; 25(17): 7900 - 7916. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. J. Cejas, L. M. Carlson, J. Zhang, S. Padmanabhan, D. Kolonias, I. Lindner, S. Haley, L. H. Boise, and K. P. Lee Protein Kinase C {beta}II Plays an Essential Role in Dendritic Cell Differentiation and Autoregulates Its Own Expression J. Biol. Chem., August 5, 2005; 280(31): 28412 - 28423. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Schutyser, A. Richmond, and J. Van Damme Involvement of CC chemokine ligand 18 (CCL18) in normal and pathological processes J. Leukoc. Biol., July 1, 2005; 78(1): 14 - 26. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Lee, S. N. Sait, and M. Wetzler Characterization of dendritic-like cells derived from t(9;22) acute lymphoblastic leukemia blasts Int. Immunol., October 1, 2004; 16(10): 1377 - 1389. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Puig-Kroger, D. Serrano-Gomez, E. Caparros, A. Dominguez-Soto, M. Relloso, M. Colmenares, L. Martinez-Munoz, N. Longo, N. Sanchez-Sanchez, M. Rincon, et al. Regulated Expression of the Pathogen Receptor Dendritic Cell-specific Intercellular Adhesion Molecule 3 (ICAM-3)-grabbing Nonintegrin in THP-1 Human Leukemic Cells, Monocytes, and Macrophages J. Biol. Chem., June 11, 2004; 279(24): 25680 - 25688. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. L. Ackerman, C. Kyritsis, R. Tampe, and P. Cresswell Early phagosomes in dendritic cells form a cellular compartment sufficient for cross presentation of exogenous antigens PNAS, October 28, 2003; 100(22): 12889 - 12894. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. Lindner, M. A. Kharfan-Dabaja, E. Ayala, D. Kolonias, L. M. Carlson, Y. Beazer-Barclay, U. Scherf, J. H. Hnatyszyn, and K. P. Lee Induced Dendritic Cell Differentiation of Chronic Myeloid Leukemia Blasts Is Associated with Down-Regulation of BCR-ABL J. Immunol., August 15, 2003; 171(4): 1780 - 1791. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. L. Ackerman and P. Cresswell Regulation of MHC Class I Transport in Human Dendritic Cells and the Dendritic-Like Cell Line KG-1 J. Immunol., April 15, 2003; 170(8): 4178 - 4188. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. J. Masterson, C. C. Sombroek, T. D. de Gruijl, Y. M. F. Graus, H. J. J. van der Vliet, S. M. Lougheed, A. J. M. van den Eertwegh, H. M. Pinedo, and R. J. Scheper MUTZ-3, a human cell line model for the cytokine-induced differentiation of dendritic cells from CD34+ precursors Blood, June 28, 2002; 100(2): 701 - 703. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Poggi, R. Carosio, A. Rubartelli, and M. R. Zocchi {beta}3-Mediated engulfment of apoptotic tumor cells by dendritic cells is dependent on CAMKII: inhibition by HIV-1 Tat J. Leukoc. Biol., March 1, 2002; 71(3): 531 - 537. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. J. Soilleux, R. Barten, and J. Trowsdale Cutting Edge: DC-SIGN; a Related Gene, DC-SIGNR; and CD23 Form a Cluster on 19p13 J. Immunol., September 15, 2000; 165(6): 2937 - 2942. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
