| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
*
Deutsches Rheuma ForschungsZentrum, Berlin, Germany; and
Institut für Pathologie, Universität Würzburg, Germany
Plasma cells are found surrounding the inflammatory infiltrates of macrophages, T, and B cells in the synovial tissue of patients with rheumatoid and reactive arthritis. This characteristic arrangement suggests that in the synovial tissue CD20+ B cells differentiate into plasma cells. To examine clonal relationships, we have used micromanipulation to separately isolate CD20+ B cells and plasma cells from single infiltrates. DNA was extracted, and from both populations the VH/VL gene repertoires was determined. The data show that in the inflamed synovial tissue activated B cells are clonally expanded. During proliferation in the network of follicular dendritic cells, V gene variants are generated by the hypermutation mechanism. Surprisingly, we do not find identical rearrangements between CD20+ B cells and plasma cells. Nevertheless, the finding of clonally related plasma cells within single infiltrates suggests that these cells underwent terminal differentiation in the synovial tissue. These results indicate that B cell differentiation in the synovial tissue is a dynamic process. Whereas CD20+ B cells may turnover rapidly, plasma cells may well be long lived and thus accumulate in the synovial tissue. The analysis of individual B cells recovered from synovial tissue opens a new way to determine the specificity of those cells that take part in the local immune reaction. This will provide new insights into the pathogenesis of chronic inflammatory diseases like rheumatoid or reactive arthritis.
This article has been cited by other articles:
|
|
 |
|
  T. Cantaert, J. Kolln, T. Timmer, T. C. van der Pouw Kraan, B. Vandooren, R. M. Thurlings, J. D. Canete, A. I. Catrina, T. Out, C. L. Verweij, et al. B Lymphocyte Autoimmunity in Rheumatoid Synovitis Is Independent of Ectopic Lymphoid Neogenesis J. Immunol., July 1, 2008; 181(1): 785 - 794. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. R. Withers, C. Fiorini, R. T. Fischer, R. Ettinger, P. E. Lipsky, and A. C. Grammer T cell dependent survival of CD20+ and CD20 plasma cells in human secondary lymphoid tissue Blood, June 1, 2007; 109(11): 4856 - 4864. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Genton, Y. Wang, S. Izui, B. Malissen, G. Delsol, G. J. Fournie, M. Malissen, and H. Acha-Orbea The Th2 Lymphoproliferation Developing in LatY136F Mutant Mice Triggers Polyclonal B Cell Activation and Systemic Autoimmunity J. Immunol., August 15, 2006; 177(4): 2285 - 2293. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Munoz-Fernandez, F. J. Blanco, C. Frecha, F. Martin, M. Kimatrai, A. C. Abadia-Molina, J. M. Garcia-Pacheco, and E. G. Olivares Follicular Dendritic Cells Are Related to Bone Marrow Stromal Cell Progenitors and to Myofibroblasts J. Immunol., July 1, 2006; 177(1): 280 - 289. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Nakamura-Kikuoka, K. Takahi, H. Tsuboi, T. Toyosaki-Maeda, M. Maeda-Tanimura, C. Wakasa, N. Kikuchi, S. Norioka, M. Iwasaki, T. Matsutani, et al. Limited VH gene usage in B-cell clones established with nurse-like cells from patients with rheumatoid arthritis Rheumatology, May 1, 2006; 45(5): 549 - 557. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Jimenez-Boj, K. Redlich, B. Turk, B. Hanslik-Schnabel, A. Wanivenhaus, A. Chott, J. S. Smolen, and G. Schett Interaction between Synovial Inflammatory Tissue and Bone Marrow in Rheumatoid Arthritis J. Immunol., August 15, 2005; 175(4): 2579 - 2588. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Zhang, K.-H. Ko, Q. L. K. Lam, C. K. C. Lo, G. Srivastava, B. Zheng, Y.-L. Lau, and L. Lu Expression and function of TNF family member B cell-activating factor in the development of autoimmune arthritis Int. Immunol., August 1, 2005; 17(8): 1081 - 1092. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Takhar, L. Smurthwaite, H. A. Coker, D. J. Fear, G. K. Banfield, V. A. Carr, S. R. Durham, and H. J. Gould Allergen Drives Class Switching to IgE in the Nasal Mucosa in Allergic Rhinitis J. Immunol., April 15, 2005; 174(8): 5024 - 5032. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Ghosh, A. C. Steere, B. D. Stollar, and B. T. Huber In Situ Diversification of the Antibody Repertoire in Chronic Lyme Arthritis Synovium J. Immunol., March 1, 2005; 174(5): 2860 - 2869. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. M. Ritchie, D. H. Gilden, R. A. Williamson, M. P. Burgoon, X. Yu, K. Helm, J. R. Corboy, and G. P. Owens Comparative Analysis of the CD19+ and CD138+ Cell Antibody Repertoires in the Cerebrospinal Fluid of Patients with Multiple Sclerosis J. Immunol., July 1, 2004; 173(1): 649 - 656. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H.-J. Kim, T. Kammertoens, M. Janke, O. Schmetzer, Z. Qin, C. Berek, and T. Blankenstein Establishment of Early Lymphoid Organ Infrastructure in Transplanted Tumors Mediated by Local Production of Lymphotoxin {alpha} and in the Combined Absence of Functional B and T Cells J. Immunol., April 1, 2004; 172(7): 4037 - 4047. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. D. Buckley Why do leucocytes accumulate within chronically inflamed joints? Rheumatology, December 1, 2003; 42(12): 1433 - 1444. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. S. Nandakumar, L. Svensson, and R. Holmdahl Collagen Type II-Specific Monoclonal Antibody-Induced Arthritis in Mice: Description of the Disease and the Influence of Age, Sex, and Genes Am. J. Pathol., November 1, 2003; 163(5): 1827 - 1837. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Galli, S. Nuti, S. Tavarini, L. Galli-Stampino, C. De Lalla, G. Casorati, P. Dellabona, and S. Abrignani CD1d-restricted Help To B Cells By Human Invariant Natural Killer T Lymphocytes J. Exp. Med., April 21, 2003; 197(8): 1051 - 1057. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Yu, G. C. Sharp, and H. Braley-Mullen Dual Roles for IFN-{gamma}, But Not for IL-4, in Spontaneous Autoimmune Thyroiditis in NOD.H-2h4 Mice J. Immunol., October 1, 2002; 169(7): 3999 - 4007. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P A Klimiuk, S Sierakowski, R Latosiewicz, J P Cylwik, B Cylwik, J Skowronski, and J Chwiecko Soluble adhesion molecules (ICAM-1, VCAM-1, and E-selectin) and vascular endothelial growth factor (VEGF) in patients with distinct variants of rheumatoid synovitis Ann Rheum Dis, September 1, 2002; 61(9): 804 - 809. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. A. Coronella, C. Spier, M. Welch, K. T. Trevor, A. T. Stopeck, H. Villar, and E. M. Hersh Antigen-Driven Oligoclonal Expansion of Tumor-Infiltrating B Cells in Infiltrating Ductal Carcinoma of the Breast J. Immunol., August 15, 2002; 169(4): 1829 - 1836. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Muller, U. E. Hopken, H. Stein, and M. Lipp Systemic immunoregulatory and pathogenic functions of homeostatic chemokine receptors J. Leukoc. Biol., July 1, 2002; 72(1): 1 - 8. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. A. Klimiuk, S. Sierakowski, R. Latosiewicz, B. Cylwik, J. Skowronski, and J. Chwiecko Serum matrix metalloproteinases and tissue inhibitors of metalloproteinases in different histological variants of rheumatoid synovitis Rheumatology, January 1, 2002; 41(1): 78 - 87. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. R. Pettit, H. Weedon, M. Ahern, S. Zehntner, I. H. Frazer, J. Slavotinek, V. Au, M. D. Smith, and R. Thomas Association of clinical, radiological and synovial immunopathological responses to anti-rheumatic treatment in rheumatoid arthritis Rheumatology, November 1, 2001; 40(11): 1243 - 1255. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Bas, P. Muzzin, T. Fulpius, N. Buchs, and T. L. Vischer Indirect evidence of intra-articular immunoglobulin G synthesis in patients with Chlamydia trachomatis reactive arthritis Rheumatology, July 1, 2001; 40(7): 801 - 805. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. M. Souto-Carneiro, H. Burkhardt, E. C. Muller, R. Hermann, A. Otto, H.-G. Kraetsch, U. Sack, A. Konig, D. Heinegard, H. K. Muller-Hermelink, et al. Human Monoclonal Rheumatoid Synovial B Lymphocyte Hybridoma with a New Disease-Related Specificity for Cartilage Oligomeric Matrix Protein J. Immunol., March 15, 2001; 166(6): 4202 - 4208. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Hjelmström Lymphoid neogenesis: de novo formation of lymphoid tissue in chronic inflammation through expression of homing chemokines J. Leukoc. Biol., March 1, 2001; 69(3): 331 - 339. [Abstract] [Full Text]
|
|
|
|
|
|
A. Brauninger, W. Yang, H.-H. Wacker, K. Rajewsky, R. Kuppers, and M.-L. Hansmann B-cell development in progressively transformed germinal centers: similarities and differences compared with classical germinal centers and lymphocyte-predominant Hodgkin disease Blood, February 1, 2001; 97(3): 714 - 719. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Shi, K. Hayashida, M. Kaneko, J. Hashimoto, T. Tomita, P. E. Lipsky, H. Yoshikawa, and T. Ochi Lymphoid Chemokine B Cell-Attracting Chemokine-1 (CXCL13) Is Expressed in Germinal Center of Ectopic Lymphoid Follicles Within the Synovium of Chronic Arthritis Patients J. Immunol., January 1, 2001; 166(1): 650 - 655. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. S. K. de Hooge, F. A. J. van de Loo, O. J. Arntz, and W. B. van den Berg Involvement of IL-6, Apart from Its Role in Immunity, in Mediating a Chronic Response during Experimental Arthritis Am. J. Pathol., December 1, 2000; 157(6): 2081 - 2091. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Odendahl, A. Jacobi, A. Hansen, E. Feist, F. Hiepe, G. R. Burmester, P. E. Lipsky, A. Radbruch, and T. Dorner Disturbed Peripheral B Lymphocyte Homeostasis in Systemic Lupus Erythematosus J. Immunol., November 15, 2000; 165(10): 5970 - 5979. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Colombo, M. Dono, P. Gazzola, S. Roncella, A. Valetto, N. Chiorazzi, G. L. Mancardi, and M. Ferrarini Accumulation of Clonally Related B Lymphocytes in the Cerebrospinal Fluid of Multiple Sclerosis Patients J. Immunol., March 1, 2000; 164(5): 2782 - 2789. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
