| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Department of Immunology, Institute of Medical Science, University of Tokyo, Tokyo, Japan
Adhesiveness of integrins is up-regulated rapidly by a number of
molecules, including growth factors, cytokines, chemokines, and other
cell surface receptors, through a mechanism termed inside-out
signaling. The inside-out signaling pathways are thought to alter
integrin affinity for ligand, or cell surface distribution of integrin
by diffusion/clustering. However, it remains to be clarified whether
any physiologically relevant agonists induce a rapid change in the
affinity of ß1 integrins and how ligand-binding affinity
is modulated upon stimulation. In this study, we reported that affinity
of ß1 integrin very late Ag-5 (VLA-5) for fibronectin was
rapidly increased in bone marrow-derived mast cells by Ag cross-linking
of Fc
RI. Ligand-binding affinity of VLA-5 was also augmented by
receptor tyrosine kinases when the phospholipase C
-1/protein kinase
C pathway was inhibited. Wortmannin suppressed induction of the high
affinity state VLA-5 in either case. Conversely, introduction of a
constitutively active p110 subunit of phosphatidylinositol 3-kinase (PI
3-kinase) increased the binding affinity for fibronectin. Failure of a
constitutively active Akt to stimulate adhesion suggested that the
affinity modulation mechanisms mediated by PI 3-kinase are distinct
from the mechanisms to control growth and apoptosis by PI 3-kinase.
Taken together, our findings demonstrated that the increase of affinity
of VLA-5 was induced by physiologically relevant stimuli and PI
3-kinase was a critical affinity modulator of
VLA-5.
This article has been cited by other articles:
|
|
 |
|
  E. Chavakis, G. Carmona, C. Urbich, S. Gottig, R. Henschler, J. M. Penninger, A. M. Zeiher, T. Chavakis, and S. Dimmeler Phosphatidylinositol-3-Kinase-{gamma} Is Integral to Homing Functions of Progenitor Cells Circ. Res., April 25, 2008; 102(8): 942 - 949. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. Thamilselvan, D. H. Craig, and M. D. Basson FAK association with multiple signal proteins mediates pressure-induced colon cancer cell adhesion via a Src-dependent PI3K/Akt pathway FASEB J, June 1, 2007; 21(8): 1730 - 1741. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Oki, J. Kitaura, K. Eto, Y. Lu, M. Maeda-Yamamoto, N. Inagaki, H. Nagai, Y. Yamanishi, H. Nakajina, H. Kumagai, et al. Integrin {alpha}IIb{beta}3 Induces the Adhesion and Activation of Mast Cells through Interaction with Fibrinogen J. Immunol., January 1, 2006; 176(1): 52 - 60. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Ortiz-Stern and C. Rosales Fc{gamma}RIIIB stimulation promotes {beta}1 integrin activation in human neutrophils J. Leukoc. Biol., May 1, 2005; 77(5): 787 - 799. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Kitaura, K. Eto, T. Kinoshita, Y. Kawakami, M. Leitges, C. A. Lowell, and T. Kawakami Regulation of Highly Cytokinergic IgE-Induced Mast Cell Adhesion by Src, Syk, Tec, and Protein Kinase C Family Kinases J. Immunol., April 15, 2005; 174(8): 4495 - 4504. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. E. Secott, T. L. Lin, and C. C. Wu Mycobacterium avium subsp. paratuberculosis Fibronectin Attachment Protein Facilitates M-Cell Targeting and Invasion through a Fibronectin Bridge with Host Integrins Infect. Immun., July 1, 2004; 72(7): 3724 - 3732. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. Lam, J. Kalesnikoff, C. W. K. Lee, V. Hernandez-Hansen, B. S. Wilson, J. M. Oliver, and G. Krystal IgE alone stimulates mast cell adhesion to fibronectin via pathways similar to those used by IgE + antigen but distinct from those used by Steel factor Blood, August 15, 2003; 102(4): 1405 - 1413. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Zhu, B. Jacobs, E. Boetticher, S. Myou, A. Meliton, H. Sano, A. T. Lambertino, N. M. Munoz, and A. R. Leff IL-5-induced integrin adhesion of human eosinophils caused by ERK1/2-mediated activation of cPLA2 J. Leukoc. Biol., November 1, 2002; 72(5): 1046 - 1053. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Lorentz, D. Schuppan, A. Gebert, M. P. Manns, and S. C. Bischoff Regulatory effects of stem cell factor and interleukin-4 on adhesion of human mast cells to extracellular matrix proteins Blood, February 1, 2002; 99(3): 966 - 972. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. L. Lupher Jr., E. A. S. Harris, C. R. Beals, L. Sui, R. C. Liddington, and D. E. Staunton Cellular Activation of Leukocyte Function-Associated Antigen-1 and Its Affinity Are Regulated at the I Domain Allosteric Site J. Immunol., August 1, 2001; 167(3): 1431 - 1439. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. L. Woods and Y. Shimizu Signaling networks regulating {beta}1 integrin-mediated adhesion of T lymphocytes to extracellular matrix J. Leukoc. Biol., June 1, 2001; 69(6): 874 - 880. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Prasad, R. S. Topping, and S. J. Decker SH2-Containing Inositol 5'-Phosphatase SHIP2 Associates with the p130Cas Adapter Protein and Regulates Cellular Adhesion and Spreading Mol. Cell. Biol., February 15, 2001; 21(4): 1416 - 1428. [Abstract] [Full Text]
|
|
|
|
|
|
R. Houtman, R. Ten Broeke, J. E. Blalock, M. Villain, A. S. Koster, and F. P. Nijkamp Attenuation of Very Late Antigen-5-Mediated Adhesion of Bone Marrow-Derived Mast Cells to Fibronectin by Peptides with Inverted Hydropathy to EF-Hands J. Immunol., January 15, 2001; 166(2): 861 - 867. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. A. Epler, R. Liu, H. Chung, N. C. Ottoson, and Y. Shimizu Regulation of {beta}1 Integrin-Mediated Adhesion by T Cell Receptor Signaling Involves ZAP-70 but Differs from Signaling Events That Regulate Transcriptional Activity J. Immunol., November 1, 2000; 165(9): 4941 - 4949. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. K. Ivins, P. D. Yurchenco, and A. D. Lander Regulation of Neurite Outgrowth by Integrin Activation J. Neurosci., September 1, 2000; 20(17): 6551 - 6560. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Katagiri, M. Hattori, N. Minato, S.-k. Irie, K. Takatsu, and T. Kinashi Rap1 Is a Potent Activation Signal for Leukocyte Function-Associated Antigen 1 Distinct from Protein Kinase C and Phosphatidylinositol-3-OH Kinase Mol. Cell. Biol., March 15, 2000; 20(6): 1956 - 1969. [Abstract] [Full Text]
|
|
|
|
 |
|
 J. M. Lu-Kuo, D. A. Fruman, D. M. Joyal, L. C. Cantley, and H. R. Katz Impaired Kit- but Not Fcepsilon RI-initiated Mast Cell Activation in the Absence of Phosphoinositide 3-Kinase p85alpha Gene Products J. Biol. Chem., February 25, 2000; 275(8): 6022 - 6029. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Kinashi, K. Katagiri, S.-i. Watanabe, B. Vanhaesebroeck, J. Downward, and K. Takatsu Distinct Mechanisms of alpha 5beta 1 Integrin Activation by Ha-Ras and R-Ras J. Biol. Chem., July 14, 2000; 275(29): 22590 - 22596. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
