| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Department of Cell Biology, Albert Einstein College of Medicine, Bronx, NY 10461
The murine Ig heavy chain (IgH) 3' regulatory region contains four enhancers: hs3A, hs1,2, hs3B, and hs4. Various studies have suggested a role for these enhancers in regulating IgH expression and class switching. Here we assess the role of hs3A and hs1,2 in these processes by exploiting a naturally occurring deletion of these enhancers from the expressed, C57BL/6 allele of the F1 pre-B cell line, 70Z/3. Equivalent µ expression in 70Z/3 and 18-81 (which has an intact 3' region) indicated that hs3A and hs1,2 were not essential for µ expression at the pre-B cell stage. To further examine the role of hs3A and hs1,2 in IgH function at the plasma cell stage, we fused 70Z/3 with the plasmacytoma NSO. Electromobility shift assay analysis of the 70Z/3-NSO hybrids revealed a transcription factor complement conducive to the activation of the 3' enhancers. Despite the lack of enhancers, hs3A and hs1,2, the level of µ RNA and protein in the 70Z/3-NSO fusion hybrids was substantially elevated relative to its pre-B parent and comparable with that observed in a number of µ-producing spleen cell hybridomas. Additionally, ELISAspot assays showed that the 70Z/3-NSO hybrid underwent spontaneous class switching in culture to IgG1 at a frequency comparable with that of most hybridomas. These results indicate that hs3A and hs1,2 are not essential for high levels of IgH expression or for spontaneous class switching in a plasma cell line.
This article has been cited by other articles:
|
|
 |
|
  Z. Ju, S. A. Volpi, R. Hassan, N. Martinez, S. L. Giannini, T. Gold, and B. K. Birshtein Evidence for Physical Interaction between the Immunoglobulin Heavy Chain Variable Region and the 3' Regulatory Region J. Biol. Chem., November 30, 2007; 282(48): 35169 - 35178. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Laurencikiene, V. Tamosiunas, and E. Severinson Regulation of {epsilon} germline transcription and switch region mutations by IgH locus 3' enhancers in transgenic mice Blood, January 1, 2007; 109(1): 159 - 167. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Labbe, A. H. Tran, and C. J. Paige Murine Model of Immune-Mediated Rejection of the Acute Lymphoblastic Leukemia 70Z/3 J. Immunol., May 1, 2006; 176(9): 5354 - 5361. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Zhou, S. Saleque, O. Ermakova, M. A. Sepulveda, Q. Yang, L. A. Eckhardt, C. L. Schildkraut, and B. K. Birshtein Changes in Replication, Nuclear Location, and Expression of the Igh Locus after Fusion of a Pre-B Cell Line with a T Cell Line J. Immunol., August 15, 2005; 175(4): 2317 - 2320. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. Volgina, P.-C. Yam, and K. L. Knight A negative regulatory element in the rabbit 3'IgH chromosomal region Int. Immunol., August 1, 2005; 17(8): 973 - 982. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. E. W. Sulentic, W. Zhang, Y. J. Na, and N. E. Kaminski 2,3,7,8-Tetrachlorodibenzo-p-dioxin, an Exogenous Modulator of the 3'{alpha} Immunoglobulin Heavy Chain Enhancer in the CH12.LX Mouse Cell Line J. Pharmacol. Exp. Ther., April 1, 2004; 309(1): 71 - 78. [Abstract] [Full Text]
|
|
|
|
 |
|
 J. Zhou, O. V. Ermakova, R. Riblet, B. K. Birshtein, and C. L. Schildkraut Replication and Subnuclear Location Dynamics of the Immunoglobulin Heavy-Chain Locus in B-Lineage Cells Mol. Cell. Biol., July 1, 2002; 22(13): 4876 - 4889. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Laurencikiene, V. Deveikaite, and E. Severinson HS1,2 Enhancer Regulation of Germline {epsilon} and {gamma}2b Promoters in Murine B Lymphocytes: Evidence for Specific Promoter-Enhancer Interactions J. Immunol., September 15, 2001; 167(6): 3257 - 3265. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
