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-Chains in Immature Thymocytes1
*
Dipartimento di Biologia e Genetica per le Scienze Mediche, Università di Milano at Department of Biological and Technological Research, San Raffaele Scientific Institute (HSR), Milan, Italy;
Unité d’Immunochimie Analytique, Département d’Immunologie, Institut Pasteur, Unité de Recherche Associée, Centre National de la Recherche Scientifique D1961, and Université Pierre et Marie Curie, Paris, France; and
Institut Necker, Institut National de la Santé et Recherche Medicale, U373, Paris, France
In recombinase-deficient (RAG-2-/-) mice,
double-negative thymocytes can be stimulated to proliferate and
differentiate by anti-CD3 Abs. CD3 molecules are expressed on the
surface of these cells in association with calnexin. In this study, we
show that 
-chains can be recovered as phosphorylated proteins in
association with phosphorylated ZAP-70 from anti-CD3-stimulated
RAG-2-/- thymocytes, even though they are not
demonstrably associated with the CD3/calnexin complex. The lack of a
physical association of dimers with the CD3 complex in
RAG-2-/- thymocytes and also in a pre-TCR-expressing cell
line, as well as the efficient association of dimers with ZAP-70 in
the RAG-2-/- thymocytes, suggest that these -chain
dimers could contribute to pre-TCR signaling. This idea is supported by
the finding that in RAG-2-/- -deficient thymocytes,
ZAP-70 and p120cbl were only weakly
phosphorylated.
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