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J Repertoire1
Department of Internal Medicine and Harold C. Simmons Arthritis Research Center, University of Texas Southwestern Medical Center, Dallas, TX 75235
To define the 
light chain repertoire in humans, a single-cell
PCR technique using genomic DNA obtained from individual peripheral B
cells was employed. Of the 30 known functional V genes, 23 were
detected in either the nonproductive or productive repertoires.
Specific V genes, including 2A2, 2B2, 1G, and 4B, were overexpressed
in the nonproductive repertoire, whereas some V genes, such as 3R,
2A2, 2B2, 1C, 1G, and 1B, were overexpressed in the productive
repertoire. Comparison of the nonproductive and productive repertoires
indicated that no V genes were positively selected, whereas a number
of V genes, including 4C, 1G, 5B, and 4B, were negatively regulated.
All four of the functional J segments were found in both
repertoires, with J7 observed most often. Evidence of terminal
deoxynucleotidyltransferase activity was noted in nearly 80% of
nonproductive VJ rearrangements, and exonuclease activity was
apparent in the majority. Despite this, the mean CDR3 length was 30
base pairs in both productive and nonproductive repertoires, suggesting
that it was tightly regulated at the molecular level. These results
have provided new insights into the dimensions of the human V
repertoire and the influences that shape it.
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