| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Departments of Medicine and Microbiology-Immunology, University of California Medical Center, San Francisco, CA 94143
Members of a subfamily of G protein-coupled receptors (GPCRs), encoded by five different endothelial differentiation genes (edgs), specifically mediate effects of lysophosphatidic acid (LPA) and sphingosine 1-phosphate (S1P) on cellular proliferation and differentiation. Mechanisms of suppression of apoptosis by LPA and S1P were studied in the Tsup-1 cultured line of human T lymphoblastoma cells, which express Edg-2 and Edg-4 GPCRs for LPA and Edg-3 and Edg-5 GPCRs for S1P. At 10-10 M to 10-7 M, both LPA and S1P protected Tsup-1 cells from apoptosis induced by Abs to Fas, CD2, and CD3 plus CD28 in combination. Apoptosis elicited by C6 ceramide was inhibited by S1P, but not by LPA, in part because ceramide suppressed expression of Edg-2 and Edg-4 surface receptors for LPA without affecting Edg-3 surface receptors for S1P. At 10-9 M to 10-7 M, LPA and S1P significantly suppressed cellular levels of the apoptosis-promoting protein Bax, without altering the levels of Bcl-xL or Bcl-2 assessed by Western blots and immunoassays. Transfections of pairs of antisense plasmids for Edg-2 plus Edg-4 and Edg-3 plus Edg-5, and hygromycin selection of transfectants with reduced expression of the respective Edg R proteins in Western blots, inhibited both protection from apoptosis and reduction in cellular levels of Bax by LPA and S1P. Thus, LPA and S1P protection from apoptosis is mediated by distinct Edg GPCRs and may involve novel effects on Bax regulatory protein.
This article has been cited by other articles:
|
|
 |
|
  C. Yang, J. Lafleur, B. R. Mwaikambo, T. Zhu, C. Gagnon, S. Chemtob, A. Di Polo, and P. Hardy The Role of Lysophosphatidic Acid Receptor (LPA1) in the Oxygen-Induced Retinal Ganglion Cell Degeneration Invest. Ophthalmol. Vis. Sci., March 1, 2009; 50(3): 1290 - 1298. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Zhao, M. J. Fernandes, M. Turgeon, S. Tancrede, J. Di Battista, P. E. Poubelle, and S. G. Bourgoin Specific and overlapping sphingosine-1-phosphate receptor functions in human synoviocytes: impact of TNF-{alpha} J. Lipid Res., November 1, 2008; 49(11): 2323 - 2337. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X. Ye Lysophospholipid signaling in the function and pathology of the reproductive system Hum. Reprod. Update, September 1, 2008; 14(5): 519 - 536. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. V. Shah, R. Zhang, R. Irby, R. Kothapalli, X. Liu, T. Arrington, B. Frank, N. H. Lee, and T. P. Loughran Jr Molecular profiling of LGL leukemia reveals role of sphingolipid signaling in survival of cytotoxic lymphocytes Blood, August 1, 2008; 112(3): 770 - 781. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. M. Murph, J. Hurst-Kennedy, V. Newton, D. N. Brindley, and H. Radhakrishna Lysophosphatidic Acid Decreases the Nuclear Localization and Cellular Abundance of the p53 Tumor Suppressor in A549 Lung Carcinoma Cells Mol. Cancer Res., November 1, 2007; 5(11): 1201 - 1211. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M.-N. Raymond, C. Bole-Feysot, Y. Banno, Z. Tanfin, and P. Robin Endothelin-1 Inhibits Apoptosis through a Sphingosine Kinase 1-Dependent Mechanism in Uterine Leiomyoma ELT3 Cells Endocrinology, December 1, 2006; 147(12): 5873 - 5882. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Rubenfeld, J. Guo, N. Sookrung, R. Chen, W. Chaicumpa, V. Casolaro, Y. Zhao, V. Natarajan, and S. Georas Lysophosphatidic acid enhances interleukin-13 gene expression and promoter activity in T cells Am J Physiol Lung Cell Mol Physiol, January 1, 2006; 290(1): L66 - L74. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Bagga, K. S. Price, D. A. Lin, D. S. Friend, K. F. Austen, and J. A. Boyce Lysophosphatidic acid accelerates the development of human mast cells Blood, December 15, 2004; 104(13): 4080 - 4087. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Schafer, B. Marg, A. Gschwind, and A. Ullrich Distinct ADAM Metalloproteinases Regulate G Protein-coupled Receptor-induced Cell Proliferation and Survival J. Biol. Chem., November 12, 2004; 279(46): 47929 - 47938. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. J. Goetzl and M. H. Graler Sphingosine 1-phosphate and its type 1 G protein-coupled receptor: trophic support and functional regulation of T Lymphocytes J. Leukoc. Biol., July 1, 2004; 76(1): 30 - 35. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
U. Reiss, B. Oskouian, J. Zhou, V. Gupta, P. Sooriyakumaran, S. Kelly, E. Wang, A. H. Merrill Jr., and J. D. Saba Sphingosine-phosphate Lyase Enhances Stress-induced Ceramide Generation and Apoptosis J. Biol. Chem., January 9, 2004; 279(2): 1281 - 1290. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M.-T. Park, J.-A Choi, M.-J. Kim, H.-D. Um, S. Bae, C.-M. Kang, C.-K. Cho, S. Kang, H. Y. Chung, Y.-S. Lee, et al. Suppression of Extracellular Signal-related Kinase and Activation of p38 MAPK Are Two Critical Events Leading to Caspase-8- and Mitochondria-mediated Cell Death in Phytosphingosine-treated Human Cancer Cells J. Biol. Chem., December 12, 2003; 278(50): 50624 - 50634. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Ikeda, K. Nagashima, M. Yanase, T. Tomiya, M. Arai, Y. Inoue, K. Tejima, T. Nishikawa, M. Omata, S. Kimura, et al. Involvement of Rho/Rho kinase pathway in regulation of apoptosis in rat hepatic stellate cells Am J Physiol Gastrointest Liver Physiol, November 1, 2003; 285(5): G880 - G886. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Wang, R. Cummings, Y. Zhao, A. Kazlauskas, J. K. S. Sham, A. Morris, S. Georas, D. N. Brindley, and V. Natarajan Involvement of Phospholipase D2 in Lysophosphatidate-induced Transactivation of Platelet-derived Growth Factor Receptor-{beta} in Human Bronchial Epithelial Cells J. Biol. Chem., October 10, 2003; 278(41): 39931 - 39940. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Jin, E. Knudsen, L. Wang, Y. Bryceson, B. Damaj, S. Gessani, and A. A. Maghazachi Sphingosine 1-phosphate is a novel inhibitor of T-cell proliferation Blood, June 15, 2003; 101(12): 4909 - 4915. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. Deng, D.-A. Wang, E. Gosmanova, L. R. Johnson, and G. Tigyi LPA protects intestinal epithelial cells from apoptosis by inhibiting the mitochondrial pathway Am J Physiol Gastrointest Liver Physiol, May 1, 2003; 284(5): G821 - G829. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Hasegawa, J. R. Erickson, G. J. Goddard, S. Yu, S. Liu, K. W. Cheng, A. Eder, K. Bandoh, J. Aoki, R. Jarosz, et al. Identification of a Phosphothionate Analogue of Lysophosphatidic Acid (LPA) as a Selective Agonist of the LPA3 Receptor J. Biol. Chem., March 28, 2003; 278(14): 11962 - 11969. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Grey, Q. Chen, K. Callon, X. Xu, I. R. Reid, and J. Cornish The Phospholipids Sphingosine-1-Phosphate and Lysophosphatidic Acid Prevent Apoptosis in Osteoblastic Cells via a Signaling Pathway Involving Gi Proteins and Phosphatidylinositol-3 Kinase Endocrinology, December 1, 2002; 143(12): 4755 - 4763. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D.-A. Wang, H. Du, J. H. Jaggar, D. N. Brindley, G. J. Tigyi, and M. A. Watsky Injury-elicited differential transcriptional regulation of phospholipid growth factor receptors in the cornea Am J Physiol Cell Physiol, December 1, 2002; 283(6): C1646 - C1654. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. W. Kim, H. M. Lee, T. H. Lee, C. Kang, H. K. Kleinman, and Y. S. Gho Extracellular Membrane Vesicles from Tumor Cells Promote Angiogenesis via Sphingomyelin Cancer Res., November 1, 2002; 62(21): 6312 - 6317. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. T. Budnik and A. K. Mukhopadhyay Lysophosphatidic Acid-Induced Nuclear Localization of Protein Kinase C {delta} in Bovine Theca Cells Stimulated with Luteinizing Hormone Biol Reprod, September 1, 2002; 67(3): 935 - 944. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. E. Coy, N. Taneja, I. Lee, C. Hecquet, J. M. Bryson, and R. B. Robey LPA is a novel lipid regulator of mesangial cell hexokinase activity and HKII isoform expression Am J Physiol Renal Physiol, August 1, 2002; 283(2): F271 - F279. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M.M. Monick and G.W. Hunninghake Activation of second messenger pathways in alveolar macrophages by endotoxin Eur. Respir. J., July 1, 2002; 20(1): 210 - 222. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Z.-Q. Jin, H.-Z. Zhou, P. Zhu, N. Honbo, D. Mochly-Rosen, R. O. Messing, E. J. Goetzl, J. S. Karliner, and M. O. Gray Cardioprotection mediated by sphingosine-1-phosphate and ganglioside GM-1 in wild-type and PKCepsilon knockout mouse hearts Am J Physiol Heart Circ Physiol, June 1, 2002; 282(6): H1970 - H1977. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X. Fang, S. Yu, J. L. Tanyi, Y. Lu, J. R. Woodgett, and G. B. Mills Convergence of Multiple Signaling Cascades at Glycogen Synthase Kinase 3: Edg Receptor-Mediated Phosphorylation and Inactivation by Lysophosphatidic Acid through a Protein Kinase C-Dependent Intracellular Pathway Mol. Cell. Biol., April 1, 2002; 22(7): 2099 - 2110. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Tigyi Selective Ligands for Lysophosphatidic Acid Receptor Subtypes: Gaining Control over the Endothelial Differentiation Gene Family Mol. Pharmacol., December 1, 2001; 60(6): 1161 - 1164. [Full Text] [PDF]
|
|
|
|
|
|
O. Cuvillier and T. Levade Sphingosine 1-phosphate antagonizes apoptosis of human leukemia cells by inhibiting release of cytochrome c and Smac/DIABLO from mitochondria Blood, November 1, 2001; 98(9): 2828 - 2836. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Zakaryan, K. Karageuzyan, L. Hovsepyan, L. Karabashyan, and G. Zakaryan Quantitative analysis of phospholipids and gangliosides in bone marrow progenitors of lymphocytes, thymocytes and mature lymphocytes in tumor-bearing animals Int. Immunol., September 1, 2001; 13(9): 1141 - 1145. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Grey, T. Banovic, D. Naot, B. Hill, K. Callon, I. Reid, and J. Cornish Lysophosphatidic Acid Is an Osteoblast Mitogen Whose Proliferative Actions Involve Gi Proteins and Protein Kinase C, But Not P42/44 Mitogen-Activated Protein Kinases Endocrinology, March 1, 2001; 142(3): 1098 - 1106. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Zheng, Y. Kong, and E. J. Goetzl Lysophosphatidic Acid Receptor-Selective Effects on Jurkat T Cell Migration Through a Matrigel Model Basement Membrane J. Immunol., February 15, 2001; 166(4): 2317 - 2322. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Xu, Y.-j. Xiao, L. M. Baudhuin, and B. M. Schwartz The Role and Clinical Applications of Bioactive Lysolipids in Ovarian Cancer Reproductive Sciences, January 1, 2001; 8(1): 1 - 13. [Abstract] [PDF]
|
|
|
|
|
|
E. J. Goetzl, Y. Kong, and J. K. Voice Cutting Edge: Differential Constitutive Expression of Functional Receptors for Lysophosphatidic Acid by Human Blood Lymphocytes J. Immunol., May 15, 2000; 164(10): 4996 - 4999. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. J. Goetzl, H. Lee, T. Azuma, T. P. Stossel, C. W. Turck, and J. S. Karliner Gelsolin Binding and Cellular Presentation of Lysophosphatidic Acid J. Biol. Chem., May 5, 2000; 275(19): 14573 - 14578. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. R. Van Brocklyn, M. H. Graler, G. Bernhardt, J. P. Hobson, M. Lipp, and S. Spiegel Sphingosine-1-phosphate is a ligand for the G protein-coupled receptor EDG-6 Blood, April 15, 2000; 95(8): 2624 - 2629. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. S. Panetti, J. Nowlen, and D. F. Mosher Sphingosine-1-Phosphate and Lysophosphatidic Acid Stimulate Endothelial Cell Migration Arterioscler Thromb Vasc Biol, April 1, 2000; 20(4): 1013 - 1019. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. An, Y. Zheng, and T. Bleu Sphingosine 1-Phosphate-induced Cell Proliferation, Survival, and Related Signaling Events Mediated by G Protein-coupled Receptors Edg3 and Edg5 J. Biol. Chem., January 7, 2000; 275(1): 288 - 296. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Olivera, T. Kohama, L. Edsall, V. Nava, O. Cuvillier, S. Poulton, and S. Spiegel Sphingosine Kinase Expression Increases Intracellular Sphingosine-1-Phosphate and Promotes Cell Growth and Survival J. Cell Biol., November 1, 1999; 147(3): 545 - 558. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. J. Goetzl, H. Dolezalova, Y. Kong, Y.-L. Hu, R. B. Jaffe, K. R. Kalli, and C. A. Conover Distinctive Expression and Functions of the Type 4 Endothelial Differentiation Gene-encoded G Protein-coupled Receptor for Lysophosphatidic Acid in Ovarian Cancer Cancer Res., October 1, 1999; 59(20): 5370 - 5375. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. J. Goetzl, H. Dolezalova, Y. Kong, and L. Zeng Dual Mechanisms for Lysophospholipid Induction of Proliferation of Human Breast Carcinoma Cells Cancer Res., September 1, 1999; 59(18): 4732 - 4737. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y.-G. Kwon, J.-K. Min, K.-M. Kim, D.-J. Lee, T. R. Billiar, and Y.-M. Kim Sphingosine 1-Phosphate Protects Human Umbilical Vein Endothelial Cells from Serum-deprived Apoptosis by Nitric Oxide Production J. Biol. Chem., March 30, 2001; 276(14): 10627 - 10633. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Banno, Y. Takuwa, Y. Akao, H. Okamoto, Y. Osawa, T. Naganawa, S. Nakashima, P.-G. Suh, and Y. Nozawa Involvement of Phospholipase D in Sphingosine 1-Phosphate-induced Activation of Phosphatidylinositol 3-Kinase and Akt in Chinese Hamster Ovary Cells Overexpressing EDG3 J. Biol. Chem., September 14, 2001; 276(38): 35622 - 35628. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z.-Q. Jin, H.-Z. Zhou, P. Zhu, N. Honbo, D. Mochly-Rosen, R. O. Messing, E. J. Goetzl, J. S. Karliner, and M. O. Gray Cardioprotection mediated by sphingosine-1-phosphate and ganglioside GM-1 in wild-type and PKCepsilon knockout mouse hearts Am J Physiol Heart Circ Physiol, June 1, 2002; 282(6): H1970 - H1977. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
