| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Department of Anatomy and Cell Biology, McGill University, Montreal, Canada
B cell development in mouse bone marrow depends critically upon IL-7. To examine the possible in vivo trophic role of IL-7, we have quantitated apoptosis and Bcl-2 family proteins in populations of phenotypically defined B lineage cells in IL-7-deficient and IL-7-overexpressing mice. Using immunofluorescence labeling, multiparameter flow cytometry, and a short-term culture assay, we show that the apoptotic rates of precursor B cells, but not of more mature B cells, are enhanced by IL-7 gene deletion, associated with increased intracellular content of Bax and decreased Bcl-2, while, conversely, an IL-7 transgene suppresses precursor B cell apoptosis and produces low Bax and high Bcl-2 levels. During normal B cell development, high Bax/Bcl-2 ratios characterize cells undergoing greatest apoptotic cell death. Pro-B cells in RAG-2-/- mice, all destined to abort, show elevated Bax levels and Bax/Bcl-2 ratios. By comparison with the elevated rate of pro-B cell apoptosis in RAG-2-/- mice, provisional estimates have been made for the fraction of pro-B cells undergoing apoptosis in normal mice (70%), IL-7-/- mice (85%), and IL-7 transgenic mice (35%). The results demonstrate that IL-7 strongly promotes in vivo cell survival and maintains antiapoptotic Bcl-2/Bax ratios during the development of precursor B cells in mouse bone marrow.
This article has been cited by other articles:
|
|
 |
|
  A. J. Giles, T. P. Bender, and K. S. Ravichandran The Adaptor Protein Shc Plays a Key Role during Early B Cell Development J. Immunol., November 1, 2009; 183(9): 5468 - 5476. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Q. L. K. Lam, C. K. C. Lo, B.-J. Zheng, K.-H. Ko, D. G. Osmond, G. E. Wu, R. Rottapel, and L. Lu Impaired V(D)J recombination and increased apoptosis among B cell precursors in the bone marrow of c-Abl-deficient mice Int. Immunol., March 1, 2007; 19(3): 267 - 276. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Ho, S. Moir, A. Malaspina, M. L. Howell, W. Wang, A. C. DiPoto, M. A. O'Shea, G. A. Roby, R. Kwan, J. M. Mican, et al. Two overrepresented B cell populations in HIV-infected individuals undergo apoptosis by different mechanisms PNAS, December 19, 2006; 103(51): 19436 - 19441. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Lu, M. S. Caplan, A. P. Saraf, D. Li, L. Adler, X. Liu, and T. Jilling Platelet-activating factor-induced apoptosis is blocked by Bcl-2 in rat intestinal epithelial cells Am J Physiol Gastrointest Liver Physiol, February 1, 2004; 286(2): G340 - G350. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S.-K. Lee, J. F. Kalinowski, S. L. Jastrzebski, L. Puddington, and J. A. Lorenzo Interleukin-7 Is a Direct Inhibitor of in Vitro Osteoclastogenesis Endocrinology, August 1, 2003; 144(8): 3524 - 3531. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Mehr, G. Shahaf, A. Sah, and M. Cancro Asynchronous differentiation models explain bone marrow labeling kinetics and predict reflux between the pre- and immature B cell pools Int. Immunol., March 1, 2003; 15(3): 301 - 312. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. J. Fry and C. L. Mackall Interleukin-7: from bench to clinic Blood, May 13, 2002; 99(11): 3892 - 3904. [Full Text] [PDF]
|
|
|
|
 |
|
 T. L. Carvalho, T. Mota-Santos, A. Cumano, J. Demengeot, and P. Vieira Arrested B Lymphopoiesis and Persistence of Activated B Cells in Adult Interleukin 7-/- Mice J. Exp. Med., October 15, 2001; 194(8): 1141 - 1150. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. C. Sandel, M. Gendelman, G. Kelsoe, and J. G. Monroe Definition of a Novel Cellular Constituent of the Bone Marrow That Regulates the Response of Immature B Cells to B Cell Antigen Receptor Engagement J. Immunol., May 15, 2001; 166(10): 5935 - 5944. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Wei, R. Zeff, and I. Goldschneider Murine Pro-B Cells Require IL-7 and Its Receptor Complex to Up-Regulate IL-7R{alpha}, Terminal Deoxynucleotidyltransferase, and c{micro} Expression J. Immunol., February 15, 2000; 164(4): 1961 - 1970. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
