| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Departments of
*
Oral Biology and
Microbiology, Immunobiology Vaccine Center, University of Alabama Medical Center, Birmingham, AL 35294;
Department of Clinical Bio-regulatory Science, Graduate School of Medicine, Kyoto University, Kyoto, Japan; and
§
Department of Mucosal Immunology, Research Institute for Microbial Diseases, Osaka University, Suita, Osaka, Japan
CD40 ligand (CD40L) gene-disrupted (CD40L-/-) mice
were employed to examine the role of costimulatory signals via
CD40L-CD40 interactions in mucosally induced tolerance.
CD40L-/- and control (CD40L+/+) mice of the
same C57BL/6 x 129/J background were immunized orally with 25 mg
of OVA before systemic challenge with OVA in CFA. While
CD40L+/+ mice showed reductions in Ag-specific T cell
responses including delayed-type hypersensitivity (DTH) and
proliferative responses, CD40L-/- mice underwent normal T
cell responses. Further, cytokine analysis of splenic CD4+
T cells showed that both Th1-type (e.g., IFN-
and IL-2) and Th2-type
(e.g., IL-4, IL-5, IL-6, and IL-10) responses were maintained in
CD40L-/- mice orally immunized with OVA, whereas these
cytokine responses in CD40L+/+ mice were significantly
reduced. In addition, splenic CD4+ T cells from
CD40L-/- mice orally immunized with OVA provided B cell
help in Ag-specific Ab-forming cells when the cells were cultured with
naive B cells in the presence of Ag and CD40L-transfected cell lines.
In contrast, an identical culture condition containing splenic
CD4+ T cells from orally tolerized CD40L+/+
mice did not exhibit helper activity. Taken together, these findings
indicate that CD40L and CD40 interactions are essential for the
induction of systemic T cell unresponsiveness to orally administered
Ag.
This article has been cited by other articles:
|
|
 |
|
  D. W. Smith and C. Nagler-Anderson Preventing Intolerance: The Induction of Nonresponsiveness to Dietary and Microbial Antigens in the Intestinal Mucosa J. Immunol., April 1, 2005; 174(7): 3851 - 3857. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. C. Tsitoura, V. P. Yeung, R. H. DeKruyff, and D. T. Umetsu Critical role of B cells in the development of T cell tolerance to aeroallergens Int. Immunol., June 1, 2002; 14(6): 659 - 667. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Nierkens, P. van Helden, M. Bol, R. Bleumink, P. van Kooten, S. Ramdien-Murli, L. Boon, and R. Pieters Selective Requirement for CD40-CD154 in Drug-Induced Type 1 Versus Type 2 Responses to Trinitrophenyl-Ovalbumin J. Immunol., April 15, 2002; 168(8): 3747 - 3754. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Fujihashi, T. Dohi, P. D. Rennert, M. Yamamoto, T. Koga, H. Kiyono, and J. R. McGhee Peyer's patches are required for oral tolerance to proteins PNAS, March 13, 2001; 98(6): 3310 - 3315. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. M. Howard and S. D. Miller Autoimmune Intervention by CD154 Blockade Prevents T Cell Retention and Effector Function in the Target Organ J. Immunol., February 1, 2001; 166(3): 1547 - 1553. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Koga, J. R. McGhee, H. Kato, R. Kato, H. Kiyono, and K. Fujihashi Evidence For Early Aging in the Mucosal Immune System J. Immunol., November 1, 2000; 165(9): 5352 - 5359. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Liu, V. K. Kuchroo, and H. L. Weiner B7.2 (CD86) But Not B7.1 (CD80) Costimulation Is Required for the Induction of Low Dose Oral Tolerance J. Immunol., August 15, 1999; 163(4): 2284 - 2290. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
