The JI
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     
 

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Request Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Sarkissian, M.
Right arrow Articles by Lafyatis, R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Sarkissian, M.
Right arrow Articles by Lafyatis, R.
Right arrowPubmed/NCBI databases
*Compound via MeSH
*Substance via MeSH
Medline Plus Health Information
*Joint Disorders
*Rheumatoid Arthritis
The Journal of Immunology, 1999, 162: 1772-1779.
Copyright © 1999 by The American Association of Immunologists

Integrin Engagement Regulates Proliferation and Collagenase Expression of Rheumatoid Synovial Fibroblasts1

Madathia Sarkissian and Robert Lafyatis2

Department of Medicine, Arthritis Center, Boston University School of Medicine, Boston, MA 02118

Growth of and metalloproteinase production by fibroblast-like synoviocytes (FLSs) in patients with rheumatoid arthritis (RA) contribute to cartilage and bone destruction associated with development of the expanding inflammatory tissue referred to as pannus. Increased levels of extracellular matrix (ECM) proteins in the pannus suggest that intracellular signals generated through integrin receptors might control these processes. We developed a cell culture system permitting accurate assessment of the effect of cell adhesion to various ECM proteins on FLS phenotype. We show that FLS proliferation to platelet-derived growth factor requires a second signal provided by adhesion to an ECM protein. Fibronectin, vitronectin, collagen, or laminin could provide the second signal and was similarly required for the proliferation of FLSs from RA or osteoarthritis patients. Adhesion to fibronectin, collagen, or Arg-Gly-Asp peptide down-regulated collagenase expression. Primarily {alpha}v integrin receptors mediated this down-regulation upon adhesion to fibronectin. Loss of cell adhesion and TNF-{alpha} stimulation synergistically increased collagenase expression. Increased collagenase expression upon nonadherence was mimicked by treatment with cytochalasin B, suggesting that the loss of cytoskeletal structure associated with a change in cell shape mediates increased collagenase in nonadherent cells. Thus, although increased fibronectin in the lining layer in RA might be expected to inhibit collagenase expression, the change in cell shape associated with this multilayer structure might actually lead to increased collagenase expression.




This article has been cited by other articles:


Home page
 Ann Rheum DisHome page
M. Hoberg, M. Rudert, T. Pap, G. Klein, S. Gay, and W. K Aicher
Attachment to laminin-111 facilitates transforming growth factor {beta}-induced expression of matrix metalloproteinase-3 in synovial fibroblasts
Ann Rheum Dis, April 1, 2007; 66(4): 446 - 451.
[Abstract] [Full Text] [PDF]

Home page
 Ann Rheum DisHome page
A Kitagawa, Y Miura, R Saura, M Mitani, H Ishikawa, A Hashiramoto, S Yoshiya, S Shiozawa, and M Kurosaka
Anchorage on fibronectin via VLA-5 ({alpha}5{beta}1 integrin) protects rheumatoid synovial cells from Fas-induced apoptosis
Ann Rheum Dis, June 1, 2006; 65(6): 721 - 727.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
S. W. Kamau, P. O. Hassa, B. Steitz, A. Petri-Fink, H. Hofmann, M. Hofmann-Amtenbrink, B. von Rechenberg, and M. O. Hottiger
Enhancement of the efficiency of non-viral gene delivery by application of pulsed magnetic field
Nucleic Acids Res., March 15, 2006; 34(5): e40 - e40.
[Abstract] [Full Text] [PDF]

Home page
 Rheumatology (Oxford)Home page
X. Chang, R. Yamada, A. Suzuki, Y. Kochi, T. Sawada, and K. Yamamoto
Citrullination of fibronectin in rheumatoid arthritis synovial tissue
Rheumatology, November 1, 2005; 44(11): 1374 - 1382.
[Abstract] [Full Text] [PDF]

Home page
 Ann Rheum DisHome page
M Neidhart, F Zaucke, R von Knoch, A Jungel, B A Michel, R E Gay, and S Gay
Galectin-3 is induced in rheumatoid arthritis synovial fibroblasts after adhesion to cartilage oligomeric matrix protein
Ann Rheum Dis, March 1, 2005; 64(3): 419 - 424.
[Abstract] [Full Text] [PDF]

Home page
 Ann Rheum DisHome page
O Sanchez-Pernaute, R Largo, E Calvo, M A Alvarez-Soria, J Egido, and G Herrero-Beaumont
A fibrin based model for rheumatoid synovitis
Ann Rheum Dis, December 1, 2003; 62(12): 1135 - 1138.
[Abstract] [Full Text] [PDF]

Home page
 Rheumatology (Oxford)Home page
O. Sanchez-Pernaute, M. J. Lopez-Armada, E. Calvo, I. Diez-Ortego, R. Largo, J. Egido, and G. Herrero-Beaumont
Fibrin generated in the synovial fluid activates intimal cells from their apical surface: a sequential morphological study in antigen-induced arthritis
Rheumatology, January 1, 2003; 42(1): 19 - 25.
[Abstract] [Full Text] [PDF]

Home page
 Ann Rheum DisHome page
T C A Tolboom, E Pieterman, W H van der Laan, R E M Toes, A L Huidekoper, R G H H Nelissen, F C Breedveld, and T W J Huizinga
Invasive properties of fibroblast-like synoviocytes: correlation with growth characteristics and expression of MMP-1, MMP-3, and MMP-10
Ann Rheum Dis, November 1, 2002; 61(11): 975 - 980.
[Abstract] [Full Text] [PDF]

Home page
 RadiologyHome page
M. Walther, H. Harms, V. Krenn, S. Radke, S. Kirschner, and F. Gohlke
Synovial Tissue of the Hip at Power Doppler US: Correlation between Vascularity and Power Doppler US Signal
Radiology, October 1, 2002; 225(1): 225 - 231.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
Y. Matsumoto, K. Tanaka, G. Hirata, M. Hanada, S. Matsuda, T. Shuto, and Y. Iwamoto
Possible Involvement of the Vascular Endothelial Growth Factor-Flt-1-Focal Adhesion Kinase Pathway in Chemotaxis and the Cell Proliferation of Osteoclast Precursor Cells in Arthritic Joints
J. Immunol., June 1, 2002; 168(11): 5824 - 5831.
[Abstract] [Full Text] [PDF]

Home page
 Ann Rheum DisHome page
D Baeten, P Demetter, C Cuvelier, F Van den Bosch, E Kruithof, N Van Damme, G Verbruggen, H Mielants, E M Veys, and F De Keyser
Comparative study of the synovial histology in rheumatoid arthritis, spondyloarthropathy, and osteoarthritis: influence of disease duration and activity
Ann Rheum Dis, December 1, 2000; 59(12): 945 - 953.
[Abstract] [Full Text]

Home page
 J. Leukoc. Biol.Home page
G. G. Vaday, R. Hershkoviz, M. A. Rahat, N. Lahat, L. Cahalon, and O. Lider
Fibronectin-bound TNF-{alpha} stimulates monocyte matrix metalloproteinase-9 expression and regulates chemotaxis
J. Leukoc. Biol., November 1, 2000; 68(5): 737 - 747.
[Abstract] [Full Text]

Home page
 J. Cell Sci.Home page
L Yan, M. Moses, S Huang, and D. Ingber
Adhesion-dependent control of matrix metalloproteinase-2 activation in human capillary endothelial cells
J. Cell Sci., January 11, 2000; 113(22): 3979 - 3987.
[Abstract] [PDF]

Home page
 Ann Rheum DisHome page
Y. T Konttinen, T. F. Li, J. W. Xu, M. Tagaki, L. Pirilä, T. Silvennoinen, S. Santavirta, and I. Virtanen
Expression of laminins and their integrin receptors in different conditions of synovial membrane and synovial membrane-like interface tissue
Ann Rheum Dis, November 1, 1999; 58(11): 683 - 690.
[Abstract] [Full Text]

Home page
 Proc. Natl. Acad. Sci. USAHome page
M. Sen, K. Lauterbach, H. El-Gabalawy, G. S. Firestein, M. Corr, and D. A. Carson
Expression and function of wingless and frizzled homologs in rheumatoid arthritis
PNAS, March 14, 2000; 97(6): 2791 - 2796.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Website Copyright © 1999 by The American Association of Immunologists, Inc. All rights reserved.
All Contents Copyright © 1999 by The American Association of Immunologists, Inc. All rights reserved.