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Department of Internal Medicine, University of Texas Southwestern Medical Center, Dallas, TX 75235
Activated T cells acquire endothelial cell (EC) plasma membrane
constituents during transendothelial migration. This was assessed using
an in vitro model system in which human peripheral blood
CD4+ T cells migrated through confluent monolayers of
HUVEC. Flow cytometry of migrated CD4+ T cells demonstrated
that activated, but not resting, T cells acquired a variety of
endothelial surface determinants, including CD31, CD49d, CD54, CD61,
and CD62E. The extracellular domains of these molecules were detected
on migrated T cells with mAbs, including those directed to the
ligand-binding regions. A number of approaches were employed to
document that the acquisition of these molecules was uniquely
accomplished by activated T cells and clearly involved transfer from
both resting and TNF-
-activated EC. Acquisition of endothelial
markers by activated T cells occurred as part of the transfer of
membrane components, as migrating T cells acquired EC membranes
prelabeled with the lipophilic dye, 3,3'-dihexadecyloxacarbocyanine
perchlorate (DiOC-16), along with EC surface proteins. Thus, during
transendothelial migration, activated T cells acquire endothelial
membrane components, and as a result may deliver them to perivascular
sites.
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