| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
,
Departments of
*
Pathology,
Medicine, and
Microbiology and Immunology, State University of New York Health Science Center, College of Medicine, Syracuse, NY 13210
Stimulation of the CD95/Fas/Apo-1 receptor leads to apoptosis
through activation of the caspase family of cysteine proteases and
disruption of the mitochondrial transmembrane potential
(
m). We show that, in Jurkat human T cells and
peripheral blood lymphocytes, Fas-induced apoptosis is preceded by 1)
an increase in reactive oxygen intermediates (ROI) and 2) an elevation
of m. These events are followed by externalization of
phosphatidylserine (PS), disruption of m, and cell
death. The caspase inhibitor peptides, DEVD-CHO, Z-VAD.fmk, and
Boc-Asp.fmk, blocked Fas-induced PS externalization, disruption of
m, and cell death, suggesting that these events are
sequelae of caspase activation. By contrast, in the presence of caspase
inhibitors, ROI levels and m of Fas-stimulated cells
remained elevated. Because ROI levels and m are
regulated by the supply of reducing equivalents from the pentose
phosphate pathway (PPP), we studied the impact of transaldolase (TAL),
a key enzyme of the PPP, on Fas signaling. Overexpression of TAL
accelerated Fas-induced mitochondrial ROI production,
m elevation, activation of caspase-8 and caspase-3,
proteolysis of poly(A)DP-ribose polymerase, and PS externalization.
Additionally, suppression of TAL diminished these activities.
Therefore, by controlling the balance between mitochondrial ROI
production and metabolic supply of reducing equivalents through the
PPP, TAL regulates susceptibility to Fas-induced apoptosis. Early
increases in ROI levels and m as well as the dominant
effect of TAL expression on activation of caspase-8/Fas-associated
death domain-like IL-1ß-converting enzyme, the most upstream member
of the caspase cascade, suggest a pivotal role for redox signaling at
the initiation of Fas-mediated apoptosis.
This article has been cited by other articles:
|
|
 |
|
  M. Sorice, P. Matarrese, A. Tinari, A. M. Giammarioli, T. Garofalo, V. Manganelli, L. Ciarlo, L. Gambardella, G. Maccari, M. Botta, et al. Raft component GD3 associates with tubulin following CD95/Fas ligation FASEB J, October 1, 2009; 23(10): 3298 - 3308. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Norell, T. Martins da Palma, A. Lesher, N. Kaur, M. Mehrotra, O. S. Naga, N. Spivey, S. Olafimihan, N. G. Chakraborty, C. Voelkel-Johnson, et al. Inhibition of Superoxide Generation upon T-Cell Receptor Engagement Rescues Mart-127-35-Reactive T Cells from Activation-Induced Cell Death Cancer Res., August 1, 2009; 69(15): 6282 - 6289. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. R. Fernandez, T. Telarico, E. Bonilla, Q. Li, S. Banerjee, F. A. Middleton, P. E. Phillips, M. K. Crow, S. Oess, W. Muller-Esterl, et al. Activation of Mammalian Target of Rapamycin Controls the Loss of TCR{zeta} in Lupus T Cells through HRES-1/Rab4-Regulated Lysosomal Degradation J. Immunol., February 15, 2009; 182(4): 2063 - 2073. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. D. Erusalimsky and S. Moncada Nitric Oxide and Mitochondrial Signaling: From Physiology to Pathophysiology Arterioscler Thromb Vasc Biol, December 1, 2007; 27(12): 2524 - 2531. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Franco, M. I. Panayiotidis, and J. A. Cidlowski Glutathione Depletion Is Necessary for Apoptosis in Lymphoid Cells Independent of Reactive Oxygen Species Formation J. Biol. Chem., October 19, 2007; 282(42): 30452 - 30465. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Bosque, J. I. Aguilo, M. A. Alava, E. Paz-Artal, J. Naval, L. M. Allende, and A. Anel The induction of Bim expression in human T-cell blasts is dependent on nonapoptotic Fas/CD95 signaling Blood, February 15, 2007; 109(4): 1627 - 1635. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Nagy, J. Ward, D. D. Mosser, A. Koncz, P. Gergely Jr., C. Stancato, Y. Qian, D. Fernandez, B. Niland, C. E. Grossman, et al. Regulation of CD4 Expression via Recycling by HRES-1/RAB4 Controls Susceptibility to HIV Infection J. Biol. Chem., November 10, 2006; 281(45): 34574 - 34591. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Perl, Y. Qian, K. R. Chohan, C. R. Shirley, W. Amidon, S. Banerjee, F. A. Middleton, K. L. Conkrite, M. Barcza, N. Gonchoroff, et al. Transaldolase is essential for maintenance of the mitochondrial transmembrane potential and fertility of spermatozoa PNAS, October 3, 2006; 103(40): 14813 - 14818. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H.-M. Shen and S. Pervaiz TNF receptor superfamily-induced cell death: redox-dependent execution FASEB J, August 1, 2006; 20(10): 1589 - 1598. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. D. Mack, A. Kapelnikov, Y. Heifetz, and M. Bender Mating-responsive genes in reproductive tissues of female Drosophila melanogaster PNAS, July 5, 2006; 103(27): 10358 - 10363. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Gong, H. Sohn, L. Xue, G. L. Firestone, and L. F. Bjeldanes 3,3'-Diindolylmethane Is a Novel Mitochondrial H+-ATP Synthase Inhibitor that Can Induce p21Cip1/Waf1 Expression by Induction of Oxidative Stress in Human Breast Cancer Cells. Cancer Res., May 1, 2006; 66(9): 4880 - 4887. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Niland, K. Banki, W. E. Biddison, and A. Perl CD8+ T Cell-Mediated HLA-A*0201-Restricted Cytotoxicity to Transaldolase Peptide 168-176 in Patients with Multiple Sclerosis J. Immunol., December 15, 2005; 175(12): 8365 - 8378. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Sim, T.-S. Yong, S.-J. Park, K.-i. Im, Y. Kong, J.-S. Ryu, D.-Y. Min, and M. H. Shin NADPH Oxidase-Derived Reactive Oxygen Species-Mediated Activation of ERK1/2 Is Required for Apoptosis of Human Neutrophils Induced by Entamoeba histolytica J. Immunol., April 1, 2005; 174(7): 4279 - 4288. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Matarrese, A. Tinari, E. Mormone, G. A. Bianco, M. A. Toscano, B. Ascione, G. A. Rabinovich, and W. Malorni Galectin-1 Sensitizes Resting Human T Lymphocytes to Fas (CD95)-mediated Cell Death via Mitochondrial Hyperpolarization, Budding, and Fission J. Biol. Chem., February 25, 2005; 280(8): 6969 - 6985. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M.-T. Park, M.-J. Kim, Y.-H. Kang, S.-Y. Choi, J.-H. Lee, J.-A Choi, C.-M. Kang, C.-K. Cho, S. Kang, S. Bae, et al. Phytosphingosine in combination with ionizing radiation enhances apoptotic cell death in radiation-resistant cancer cells through ROS-dependent and -independent AIF release Blood, February 15, 2005; 105(4): 1724 - 1733. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. I. Pozniakovsky, D. A. Knorre, O. V. Markova, A. A. Hyman, V. P. Skulachev, and F. F. Severin Role of mitochondria in the pheromone- and amiodarone-induced programmed death of yeast J. Cell Biol., January 17, 2005; 168(2): 257 - 269. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Mehrotra, A. Chhabra, S. Chattopadhyay, D. I. Dorsky, N. G. Chakraborty, and B. Mukherji Rescuing Melanoma Epitope-Specific Cytolytic T Lymphocytes from Activation-Induced Cell Death, by SP600125, an Inhibitor of JNK: Implications in Cancer Immunotherapy J. Immunol., November 15, 2004; 173(10): 6017 - 6024. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Nagy, M. Barcza, N. Gonchoroff, P. E. Phillips, and A. Perl Nitric Oxide-Dependent Mitochondrial Biogenesis Generates Ca2+ Signaling Profile of Lupus T Cells J. Immunol., September 15, 2004; 173(6): 3676 - 3683. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Sato, T. Machida, S. Takahashi, S. Iyama, Y. Sato, K. Kuribayashi, K. Takada, T. Oku, Y. Kawano, T. Okamoto, et al. Fas-Mediated Apoptosome Formation Is Dependent on Reactive Oxygen Species Derived from Mitochondrial Permeability Transition in Jurkat Cells J. Immunol., July 1, 2004; 173(1): 285 - 296. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Shimizu, T. Numata, and Y. Okada A role of reactive oxygen species in apoptotic activation of volume-sensitive Cl- channel PNAS, April 27, 2004; 101(17): 6770 - 6773. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. E. Grossman, Y. Qian, K. Banki, and A. Perl ZNF143 Mediates Basal and Tissue-specific Expression of Human Transaldolase J. Biol. Chem., March 26, 2004; 279(13): 12190 - 12205. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Porras, S. Zuluaga, A. Valladares, A. M. Alvarez, B. Herrera, I. Fabregat, and M. Benito Long-Term Treatment with Insulin Induces Apoptosis in Brown Adipocytes: Role of Oxidative Stress Endocrinology, December 1, 2003; 144(12): 5390 - 5401. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Nagy, A. Koncz, and A. Perl T Cell Activation-Induced Mitochondrial Hyperpolarization Is Mediated by Ca2+- and Redox-Dependent Production of Nitric Oxide J. Immunol., November 15, 2003; 171(10): 5188 - 5197. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Kurosaka, M. Takahashi, N. Watanabe, and Y. Kobayashi Silent Cleanup of Very Early Apoptotic Cells by Macrophages J. Immunol., November 1, 2003; 171(9): 4672 - 4679. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Tsuruya, M. Tokumoto, T. Ninomiya, M. Hirakawa, K. Masutani, M. Taniguchi, K. Fukuda, H. Kanai, H. Hirakata, and M. Iida Antioxidant ameliorates cisplatin-induced renal tubular cell death through inhibition of death receptor-mediated pathways Am J Physiol Renal Physiol, August 1, 2003; 285(2): F208 - F218. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Perez-Cruz, J. M. Carcamo, and D. W. Golde Vitamin C inhibits FAS-induced apoptosis in monocytes and U937 cells Blood, July 1, 2003; 102(1): 336 - 343. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Gouaze, N. Andrieu-Abadie, O. Cuvillier, S. Malagarie-Cazenave, M.-F. Frisach, M.-E. Mirault, and T. Levade Glutathione Peroxidase-1 Protects from CD95-induced Apoptosis J. Biol. Chem., November 1, 2002; 277(45): 42867 - 42874. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Bitzer, S. Armeanu, F. Prinz, G. Ungerechts, W. Wybranietz, M. Spiegel, C. Bernlohr, F. Cecconi, M. Gregor, W. J. Neubert, et al. Caspase-8 and Apaf-1-independent Caspase-9 Activation in Sendai Virus-infected Cells J. Biol. Chem., August 9, 2002; 277(33): 29817 - 29824. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Gergely Jr., B. Niland, N. Gonchoroff, R. Pullmann Jr., P. E. Phillips, and A. Perl Persistent Mitochondrial Hyperpolarization, Increased Reactive Oxygen Intermediate Production, and Cytoplasmic Alkalinization Characterize Altered IL-10 Signaling in Patients with Systemic Lupus Erythematosus J. Immunol., July 15, 2002; 169(2): 1092 - 1101. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Beltran, M. Quintero, E. Garcia-Zaragoza, E. O'Connor, J. V. Esplugues, and S. Moncada Inhibition of mitochondrial respiration by endogenous nitric oxide: A critical step in Fas signaling PNAS, June 25, 2002; 99(13): 8892 - 8897. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Huo, R.-H. Luo, S. A. Metz, and G. Li Activation of Caspase-2 Mediates the Apoptosis Induced by GTP-Depletion in Insulin-Secreting (HIT-T15) Cells Endocrinology, May 1, 2002; 143(5): 1695 - 1704. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. Droge Free Radicals in the Physiological Control of Cell Function Physiol Rev, January 1, 2002; 82(1): 47 - 95. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Jackle, C. Hasel, I. Melzner, S. Bruderlein, P. M. Jehle, and P. Moller Sustained hyposmotic stress induces cell death: apoptosis by defeat Am J Physiol Cell Physiol, November 1, 2001; 281(5): C1716 - C1726. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. V. Krysko, F. Roels, L. Leybaert, and K. D'Herde Mitochondrial Transmembrane Potential Changes Support the Concept of Mitochondrial Heterogeneity During Apoptosis J. Histochem. Cytochem., October 1, 2001; 49(10): 1277 - 1284. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Beltran, A. Mathur, M. R. Duchen, J. D. Erusalimsky, and S. Moncada The effect of nitric oxide on cell respiration: A key to understanding its role in cell survival or death PNAS, December 19, 2000; 97(26): 14602 - 14607. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. G. Heerdt, M. A. Houston, J. M. Mariadason, and L. H. Augenlicht Dissociation of Staurosporine-induced Apoptosis from G2-M Arrest in SW620 Human Colonic Carcinoma Cells: Initiation of the Apoptotic Cascade Is Associated with Elevation of the Mitochondrial Membrane Potential ({{Delta}}{{psi}}m) Cancer Res., December 1, 2000; 60(23): 6704 - 6713. [Abstract] [Full Text]
|
|
|
|
 |
|
 V. J. Thannickal and B. L. Fanburg Reactive oxygen species in cell signaling Am J Physiol Lung Cell Mol Physiol, December 1, 2000; 279(6): L1005 - L1028. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. D. S. Jean, C. Debbasch, M. Rahmani, F. Brignole, G. Feldmann, J.-M. Warnet, and C. Baudouin Fas- and Interferon {gamma}-Induced Apoptosis in Chang Conjunctival Cells: Further Investigations Invest. Ophthalmol. Vis. Sci., August 1, 2000; 41(9): 2531 - 2543. [Abstract] [Full Text]
|
|
|
|
 |
|
 E. Gottlieb, M. G. Vander Heiden, and C. B. Thompson Bcl-xL Prevents the Initial Decrease in Mitochondrial Membrane Potential and Subsequent Reactive Oxygen Species Production during Tumor Necrosis Factor Alpha-Induced Apoptosis Mol. Cell. Biol., August 1, 2000; 20(15): 5680 - 5689. [Abstract] [Full Text]
|
|
|
|
|
|
F. PUSKAS, P. GERGELY JR, K. BANKI, and A. PERL Stimulation of the pentose phosphate pathway and glutathione levels by dehydroascorbate, the oxidized form of vitamin C FASEB J, July 1, 2000; 14(10): 1352 - 1361. [Abstract] [Full Text]
|
|
|
|
|
|
A. Perl, E. Colombo, E. Samoilova, M. C. Butler, and K. Banki Human Transaldolase-associated Repetitive Elements Are Transcribed by RNA Polymerase III J. Biol. Chem., March 15, 2000; 275(10): 7261 - 7272. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Shimizu and Y. Tsujimoto Proapoptotic BH3-only Bcl-2 family members induce cytochrome c release, but not mitochondrial membrane potential loss, and do not directly modulate voltage-dependent anion channel activity PNAS, January 18, 2000; 97(2): 577 - 582. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. R. Khaled, D. A. Reynolds, H. A. Young, C. B. Thompson, K. Muegge, and S. K. Durum Interleukin-3 Withdrawal Induces an Early Increase in Mitochondrial Membrane Potential Unrelated to the Bcl-2 Family. ROLES OF INTRACELLULAR pH, ADP TRANSPORT, AND F0F1-ATPase J. Biol. Chem., February 23, 2001; 276(9): 6453 - 6462. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Almeida, J. Almeida, J. P. Bolanos, and S. Moncada Different responses of astrocytes and neurons to nitric oxide: The role of glycolytically generated ATP in astrocyte protection PNAS, December 18, 2001; 98(26): 15294 - 15299. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
