| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Transplant Research Division, Toronto Hospital, Toronto, Ontario, Canada
We reported that hepatic mononuclear, nonparenchymal cells (NPC)
can inhibit the immune response seen when allogeneic C57BL/6 dendritic
cells (DC) are incubated with C3H spleen responder cells. Cells derived
from these cultures transfer increased survival of C57BL/6 renal
allografts in C3H mice. We also found that increased expression of OX-2
on DC was associated with inhibition of cytokine production and renal
allograft rejection. We explored whether inhibition by hepatic NPC was
a function of OX-2 expression by these cells. Fresh C57BL/6
spleen-derived DC were cultured with C3H spleen responder cells and
other putative coregulatory cells. The latter were derived from fresh
C3H or C57BL/6 liver NPC, or from C3H or C57BL/6 mice treated for 10
days by i.v. infusion of human Flt3 ligand. Different
populations of murine bone marrow-derived DC from cultures of bone
marrow with IL-4 plus granulocyte-macrophage-CSF were also used as a
source of putative regulator cells. Supernatants of all stimulated
cultures were examined for functional expression of different cytokines
(IL-2, IL-4, IFN-
, and TGFß). We found that fresh C57BL/6 splenic
DC induced IL-2, not IL-4, production. Cells from the sources indicated
inhibited IL-2 and IFN- production and promoted IL-4 and TGFß
production. Inhibition was associated with increased expression of OX-2
on these cells, as defined by semiquantitative PCR and FACS analysis.
By size fractionation, cells expressing OX-2 were a subpopulation of
NLDC145+ cells. Our data imply a role for cells expressing
OX-2 in the regulation of induction of cytokine production by
conventional allostimulatory DC.
This article has been cited by other articles:
|
|
 |
|
  R. Gorczynski, I. Khatri, L. Lee, and I. Boudakov An Interaction between CD200 and Monoclonal Antibody Agonists to CD200R2 in Development of Dendritic Cells That Preferentially Induce Populations of CD4+CD25+ T Regulatory Cells J. Immunol., May 1, 2008; 180(9): 5946 - 5955. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Kretz-Rommel, F. Qin, N. Dakappagari, E. P. Ravey, J. McWhirter, D. Oltean, S. Frederickson, T. Maruyama, M. A. Wild, M.-J. Nolan, et al. CD200 Expression on Tumor Cells Suppresses Antitumor Immunity: New Approaches to Cancer Immunotherapy J. Immunol., May 1, 2007; 178(9): 5595 - 5605. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. M. Cameron, J. W. Barrett, L. Liu, A. R. Lucas, and G. McFadden Myxoma Virus M141R Expresses a Viral CD200 (vOX-2) That Is Responsible for Down-Regulation of Macrophage and T-Cell Activation In Vivo J. Virol., May 15, 2005; 79(10): 6052 - 6067. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. D. Rosenblum, E. Olasz, J. E. Woodliff, B. D. Johnson, M. C. Konkol, K. A. Gerber, R. J. Orentas, G. Sandford, and R. L. Truitt CD200 is a novel p53-target gene involved in apoptosis-associated immune tolerance Blood, April 1, 2004; 103(7): 2691 - 2698. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A H Lau and A W Thomson Dendritic cells and immune regulation in the liver Gut, February 1, 2003; 52(2): 307 - 314. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y.-H. Chung, R. E. Means, J.-K. Choi, B.-S. Lee, and J. U. Jung Kaposi's Sarcoma-Associated Herpesvirus OX2 Glycoprotein Activates Myeloid-Lineage Cells To Induce Inflammatory Cytokine Production J. Virol., April 16, 2002; 76(10): 4688 - 4698. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. A. Clark, J.-W. Ding, G. Yu, G. A. Levy, and R. M. Gorczynski Fgl2 prothrombinase expression in mouse trophoblast and decidua triggers abortion but may be countered by OX-2 Mol. Hum. Reprod., February 1, 2001; 7(2): 185 - 194. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. D. Dick, C. Broderick, J. V. Forrester, and G. J. Wright Distribution of OX2 Antigen and OX2 Receptor within Retina Invest. Ophthalmol. Vis. Sci., January 1, 2001; 42(1): 170 - 176. [Abstract] [Full Text]
|
|
|
|
|
|
R. M. Gorczynski, K. Yu, and D. Clark Receptor Engagement on Cells Expressing a Ligand for the Tolerance-Inducing Molecule OX2 Induces an Immunoregulatory Population That Inhibits Alloreactivity In Vitro and In Vivo J. Immunol., November 1, 2000; 165(9): 4854 - 4860. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. M. Gorczynski, Z. Chen, D. A. Clark, J. Hu, G. Yu, X. Li, W. Tsang, and S. Hadidi Regulation of Gene Expression of Murine MD-1 Regulates Subsequent T Cell Activation and Cytokine Production J. Immunol., August 15, 2000; 165(4): 1925 - 1932. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. M. Gorczynski, M. S. Cattral, Z. Chen, J. Hu, J. Lei, W.-P. Min, G. Yu, and J. Ni An Immunoadhesin Incorporating the Molecule OX-2 Is a Potent Immunosuppressant That Prolongs Allo- and Xenograft Survival J. Immunol., August 1, 1999; 163(3): 1654 - 1660. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
