HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Leong, L. Y. W. Articles by Butcher, G. W. Search for Related Content PubMed PubMed Citation Articles by Leong, L. Y. W. Articles by Butcher, G. W.

RT1-U: Identification of a Novel, Active, Class Ib Alloantigen of the Rat MHC^{1 ,2}

Louise Y. W. Leong^*, Anne-France Le Rolle^*, Edward V. Deverson^*, Simon J. Powis, Audrey P. Larkins^*, John T. Vaage, Aasa Stokland, Doris Lambracht-Washington^§, Bent Rolstad, Etienne Joly^* and Geoffrey W. Butcher^3,*

^* Department of Immunology, The Babraham Institute, Cambridge, United Kingdom; Department of Biochemistry, University of Dundee, Dundee, United Kingdom; Department of Anatomy, University of Oslo, Blindern, Norway; and ^§ Transplantationslabor, Medizinische Hochschule, Hannover, Germany

In common with other mammalian species, the laboratory rat (Rattus norvegicus) expresses MHC class I molecules that have been categorized as either classical (class Ia) or nonclassical (class Ib). This distinction separates the class Ia molecules that play a conventional role in peptide Ag presentation to CD8 T cells from the others, whose function is unconventional or undefined. The class Ia molecules are encoded by the RT1-A region of the rat MHC, while the RT1-C/E/M region encodes up to 60 other class I genes or gene fragments, a number of which are known to be expressed (or to be expressible). Here we report upon novel MHC class Ib genes of the rat that we have expression cloned using new monoclonal alloantibodies and which we term RT1-U. The products detected by these Abs were readily identifiable by two-dimensional analysis of immunoprecipitates and were shown to be distinct from the class Ia products. Cellular studies of these molecules indicate that they function efficiently as targets for cytotoxic killing by appropriately raised polyclonal alloreactive CTL populations. The sequences of these class Ib genes group together in phylogenetic analysis, suggesting a unique locus or family. The combined serological, CTL, and sequence data all indicate that these products are genetically polymorphic.

This article has been cited by other articles:

				U. P. Kodavanti, M. C. Schladweiler, A. D. Ledbetter, R. V. Ortuno, M. Suffia, P. Evansky, J. H. Richards, R. H. Jaskot, R. Thomas, E. Karoly, et al. The Spontaneously Hypertensive Rat: An Experimental Model of Sulfur Dioxide-Induced Airways Disease Toxicol. Sci., November 1, 2006; 94(1): 193 - 205. [Abstract] [Full Text] [PDF]

				K. J. Lavender, B. J. Ma, E. T. Silver, and K. P. Kane The Rat RT1-A1c MHC Molecule Is a Xenogeneic Ligand Recognized by the Mouse Activating Ly-49W and Inhibitory Ly-49G Receptors J. Immunol., March 15, 2004; 172(6): 3518 - 3526. [Abstract] [Full Text] [PDF]

				A. L. Gonzalez-Munoz, A.-F. Le Rolle, H. Brun, H. J. Hedrich, D. Wedekind, S. J. Powis, E. Joly, and G. W. Butcher A Novel Instance of Class I Modification (cim) Affecting Two of Three Rat Class I RT1-A Molecules Within One MHC Haplotype J. Immunol., July 1, 2003; 171(1): 274 - 284. [Abstract] [Full Text] [PDF]

				C. Naper, S. Hayashi, L. Kveberg, E. C. Niemi, L. L. Lanier, J. T. Vaage, and J. C. Ryan Ly-49s3 Is a Promiscuous Activating Rat NK Cell Receptor for Nonclassical MHC Class I-Encoded Target Ligands J. Immunol., July 1, 2002; 169(1): 22 - 30. [Abstract] [Full Text] [PDF]

				N. M. McKechnie, W. Gurr, H. Yamada, D. Copland, and G. Braun Antigenic Mimicry: Onchocerca volvulus Antigen-Specific T Cells and Ocular Inflammation Invest. Ophthalmol. Vis. Sci., February 1, 2002; 43(2): 411 - 418. [Abstract] [Full Text] [PDF]

				S. Ioannidu, L. Walter, R. Dressel, and E. Gunther Physical Map and Expression Profile of Genes of the Telomeric Class I Gene Region of the Rat MHC J. Immunol., March 15, 2001; 166(6): 3957 - 3965. [Abstract] [Full Text] [PDF]

				N. Torres-Nagel, B. Mehling, A.-F. LeRolle, E. Joly, and T. Hunig Genetic control of peripheral TCRAV usage by representation in the preselection repertoire and MHC allele-specific overselection Int. Immunol., January 1, 2001; 13(1): 63 - 73. [Abstract] [Full Text] [PDF]

				O. Planz, T. Dumrese, S. Hulpusch, M. Schirle, S. Stevanovic, and L. Stitz A Naturally Processed Rat Major Histocompatibility Complex Class I-associated Viral Peptide as Target Structure of Borna Disease Virus-specific CD8+ T Cells J. Biol. Chem., April 20, 2001; 276(17): 13689 - 13694. [Abstract] [Full Text] [PDF]