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Intracellular Expression and Release of FcRI by Human Eosinophils¹

Department of Medicine, Division of Clinical Immunology, Johns Hopkins Asthma and Allergy Center, Baltimore, MD 21224

Although FcR have been detected on human eosinophils, levels varied from moderate to extremely low or undetectable depending on the donor and methods used. We have attempted to resolve the conflicting data by measuring levels of IgE, FcRI, and FcRII in or on human eosinophils from a variety of donors (n = 26) and late-phase bronchoalveolar lavage fluids (n = 5). Our results demonstrated little or no cell surface IgE or IgE receptors as analyzed by immunofluorescence and flow cytometry. Culture of eosinophils for up to 11 days in the presence or absence of IgE and/or IL-4 (conditions that enhance FcR on other cells) failed to induce any detectable surface FcR. However, immunoprecipitation and Western blot analysis of eosinophil lysates using mAb specific for FcRI showed a distinct band of approximately 50 kDa, similar to that found in basophils. Western blotting also showed the presence of FcR -chain, but no FcRIß. Surface biotinylation followed by immunoprecipitation again failed to detect surface FcRI, although surface FcR was easily detected. Since we were able to detect intracellular FcRI, we examined its release from eosinophils. Immunoprecipitation and Western blotting demonstrated the release of FcRI into the supernatant of cultured eosinophils, peaking at approximately 48 h. We conclude that eosinophils possess a sizable intracellular pool of FcRI that is available for release, with undetectable surface levels in a variety of subjects, including those with eosinophilia and elevated serum IgE. The biological relevance of this soluble form of FcRI remains to be determined.

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