The JI
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     
 

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Request Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kotovuori, A.
Right arrow Articles by Gahmberg, C. G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kotovuori, A.
Right arrow Articles by Gahmberg, C. G.
The Journal of Immunology, 1999, 162: 6613-6620.
Copyright © 1999 by The American Association of Immunologists

ICAM-2 and a Peptide from Its Binding Domain Are Efficient Activators of Leukocyte Adhesion and Integrin Affinity1

Annika Kotovuori, Tiina Pessa-Morikawa, Pekka Kotovuori, Pekka Nortamo and Carl G. Gahmberg2

Department of Biosciences, Division of Biochemistry, University of Helsinki, Helsinki, Finland

Cell adhesion mediated by the CD11/CD18 integrins and their ligands, the ICAMs, is required for many leukocyte functions. In resting cells the integrins are nonadhesive, but when activated they become adhesive for their ligands. Previous findings have shown that a peptide derived from the first Ig domain of ICAM-2 (P1) binds to LFA-1 (CD11a/CD18) and Mac-1 (CD11b/CD18) and activates leukocyte aggregation. Because its mechanism of action has remained poorly understood, we have now studied the peptide-induced ligand binding in detail. Here we show that P1 was able to induce CD11/CD18-dependent adhesion of human T lymphocytes to immobilized, purified ICAM-1, -2, and -3. The optimal peptide concentration was 150 µg/ml, whereas concentrations higher than 400 µg/ml did not have any stimulatory effect. The increase in adhesion was detectable within 10 min of treatment with the peptide; it was dependent on energy, divalent cations, temperature, and an intact cytoskeleton but was unaffected by protein kinase C and protein tyrosine kinase inhibitors. Peptide treatment resulted in strong stimulation of the binding of soluble, recombinant ICAMs to T lymphocytes, showing that the integrin affinity toward its ligands was increased. Importantly, soluble ICAM-2Fc was also able to induce T lymphocyte adhesion to purified ICAM-1, -2, and -3, and it was a more potent stimulatory molecule than ICAM-1Fc or ICAM-3Fc.




This article has been cited by other articles:


Home page
 BloodHome page
L. Tian, J. Lappalainen, M. Autero, S. Hanninen, H. Rauvala, and C. G. Gahmberg
Shedded neuronal ICAM-5 suppresses T-cell activation
Blood, April 1, 2008; 111(7): 3615 - 3625.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. M. Nurmi, M. Autero, A. K. Raunio, C. G. Gahmberg, and S. C. Fagerholm
Phosphorylation of the LFA-1 Integrin beta2-Chain on Thr-758 Leads to Adhesion, Rac-1/Cdc42 Activation, and Stimulation of CD69 Expression in Human T Cells
J. Biol. Chem., January 12, 2007; 282(2): 968 - 975.
[Abstract] [Full Text] [PDF]

Home page
 JCBHome page
S. C. Fagerholm, T. J. Hilden, S. M. Nurmi, and C. G. Gahmberg
Specific integrin {alpha} and {beta} chain phosphorylations regulate LFA-1 activation through affinity-dependent and -independent mechanisms
J. Cell Biol., November 21, 2005; 171(4): 705 - 715.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
O. D. Perez, D. Mitchell, G. C. Jager, and G. P. Nolan
LFA-1 signaling through p44/42 is coupled to perforin degranulation in CD56+CD8+ natural killer cells
Blood, August 15, 2004; 104(4): 1083 - 1093.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Cancer Res.Home page
H. Tanaka, M. Yashiro, T. Sunami, Y. Sakate, K. Kosaka, and K. Hirakawa
ICAM-2 Gene Therapy for Peritoneal Dissemination of Scirrhous Gastric Carcinoma
Clin. Cancer Res., July 15, 2004; 10(14): 4885 - 4892.
[Abstract] [Full Text] [PDF]

Home page
 NeurologyHome page
P. J. Lindsberg, J. Launes, L. Tian, H. Valimaa, V. Subramanian, J. Siren, L. Hokkanen, T. Hyypia, O. Carpen, and C. G. Gahmberg
Release of soluble ICAM-5, a neuronal adhesion molecule, in acute encephalitis
Neurology, February 12, 2002; 58(3): 446 - 451.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
M. D. Silverman, D. O. Zamora, Y. Pan, P. V. Texeira, S. R. Planck, and J. T. Rosenbaum
Cell Adhesion Molecule Expression in Cultured Human Iris Endothelial Cells
Invest. Ophthalmol. Vis. Sci., November 1, 2001; 42(12): 2861 - 2866.
[Abstract] [Full Text] [PDF]

Home page
 JCBHome page
E. Koivunen, T.-M. Ranta, A. Annila, S. Taube, A. Uppala, M. Jokinen, G. van Willigen, E. Ihanus, and C. G. Gahmberg
Inhibition of {beta}2Integrin-Mediated Leukocyte Cell Adhesion by Leucine-Leucine-Glycine Motif-Containing Peptides
J. Cell Biol., May 28, 2001; 153(5): 905 - 916.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
F McLaughlin, V. Ludbrook, I Kola, C. Campbell, and A. Randi
Characterisation of the tumour necrosis factor (TNF)-(alpha) response elements in the human ICAM-2 promoter
J. Cell Sci., January 12, 1999; 112(24): 4695 - 4703.
[Abstract] [PDF]

Home page
 J. Biol. Chem.Home page
S. Fagerholm, N. Morrice, C. G. Gahmberg, and P. Cohen
Phosphorylation of the Cytoplasmic Domain of the Integrin CD18 Chain by Protein Kinase C Isoforms in Leukocytes
J. Biol. Chem., January 11, 2002; 277(3): 1728 - 1738.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Website Copyright © 1999 by The American Association of Immunologists, Inc. All rights reserved.
All Contents Copyright © 1999 by The American Association of Immunologists, Inc. All rights reserved.