| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
*
Laboratory of Experimental Surgery, Surgical Department, S. Raffaele Scientific Institute, Milan, Italy;
Department of Immunology and Cell Biology, "Mario Negri" Institute, Milan, Italy;
Laboratory of Analysis, San Luigi Centre, S. Raffaele Scientific Institute, Milan, Italy; and
§
University of Milan, Milan, Italy
Because dendritic cells (DC) play a major role in the initiation of
T cell-mediated immunity, we studied the effects of glucocorticoids,
well-known inhibitors of the immune and inflammatory response, on the
differentiation and maturation of human DC. DC were differentiated from
human monocytes by culture with GM-CSF and IL-4 for 7 days with and
without dexamethasone (Dex). Cells treated with Dex (10-8
M) (Dex-DC) developed a characteristic dendritic morphology; however,
membrane phenotype analysis demonstrated that they were not fully
differentiated. Dex-DC expressed low levels of CD1a and, unlike
untreated cells, high levels of CD14 and CD16. Molecules involved in Ag
presentation (CD40, CD86, CD54) were also impaired. In contrast,
molecules involved in Ag uptake (mannose receptor, CD32) and cell
adhesion (CD11/CD18, CD54) were up-regulated. After exposure to TNF-
or CD40 ligand, Dex-DC expressed lower levels of CD83 and CD86 than
untreated cells. Dex-DC showed a higher endocytic activity, a lower APC
function, and a lower capacity to secrete cytokines than untreated
cells. Overall, these results indicate that DC differentiated in the
presence of Dex are at a more immature stage. Moreover, Dex also
partially blocked terminal maturation of already differentiated DC. In
conclusion, our data suggest that glucocorticoids may act at the very
first step of the immune response by modulating DC differentiation,
maturation, and function.
This article has been cited by other articles:
|
|
 |
|
  C. Luther, E. Adamopoulou, C. Stoeckle, V. Brucklacher-Waldert, D. Rosenkranz, L. Stoltze, S. Lauer, S. Poeschel, A. Melms, and E. Tolosa Prednisolone Treatment Induces Tolerogenic Dendritic Cells and a Regulatory Milieu in Myasthenia Gravis Patients J. Immunol., July 15, 2009; 183(2): 841 - 848. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Rayment, T. W. Kooij, W. Zhang, C. Siebold, M. F. Murphy, D. Allen, N. Willcox, and D. J. Roberts Evidence for the Specificity for Platelet HPA-1a Alloepitope and the Presenting HLA-DR52a of Diverse Antigen-Specific Helper T Cell Clones from Alloimmunized Mothers J. Immunol., July 1, 2009; 183(1): 677 - 686. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Szeles, G. Keresztes, D. Torocsik, Z. Balajthy, L. Krenacs, S. Poliska, A. Steinmeyer, U. Zuegel, M. Pruenster, A. Rot, et al. 1,25-Dihydroxyvitamin D3 Is an Autonomous Regulator of the Transcriptional Changes Leading to a Tolerogenic Dendritic Cell Phenotype J. Immunol., February 15, 2009; 182(4): 2074 - 2083. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. E. Anderson, D. J. Swan, B. L. Sayers, R. A. Harry, A. M. Patterson, A. von Delwig, J. H. Robinson, J. D. Isaacs, and C. M. U. Hilkens LPS activation is required for migratory activity and antigen presentation by tolerogenic dendritic cells J. Leukoc. Biol., February 1, 2009; 85(2): 243 - 250. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Kang, L. Xu, B. Wang, A. Chen, and G. Zheng Cutting Edge: Immunosuppressant as Adjuvant for Tolerogenic Immunization J. Immunol., April 15, 2008; 180(8): 5172 - 5176. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Chakraverty and M. Sykes The role of antigen-presenting cells in triggering graft-versus-host disease and graft-versus-leukemia Blood, July 1, 2007; 110(1): 9 - 17. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. E. Tschoep and E. Noessner Understand tolerogenic dendritic cells Blood, May 1, 2007; 109(9): 3616 - 3616. [Full Text] [PDF]
|
|
|
|
|
|
M. Bros, F. Jahrling, A. Renzing, N. Wiechmann, N.-A. Dang, A. Sutter, R. Ross, J. Knop, S. Sudowe, and A. B. Reske-Kunz A newly established murine immature dendritic cell line can be differentiated into a mature state, but exerts tolerogenic function upon maturation in the presence of glucocorticoid Blood, May 1, 2007; 109(9): 3820 - 3829. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Penna, A. Roncari, S. Amuchastegui, K. C. Daniel, E. Berti, M. Colonna, and L. Adorini Expression of the inhibitory receptor ILT3 on dendritic cells is dispensable for induction of CD4+Foxp3+ regulatory T cells by 1,25-dihydroxyvitamin D3 Blood, November 15, 2005; 106(10): 3490 - 3497. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J A Hardin Dendritic cells: potential triggers of autoimmunity and targets for therapy Ann Rheum Dis, November 1, 2005; 64(suppl_4): iv86 - iv90. [Full Text] [PDF]
|
|
|
|
|
|
L. Freeman, M. Hewison, S. V. Hughes, K. N. Evans, D. Hardie, T. K. Means, and R. Chakraverty Expression of 11{beta}-hydroxysteroid dehydrogenase type 1 permits regulation of glucocorticoid bioavailability by human dendritic cells Blood, September 15, 2005; 106(6): 2042 - 2049. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Chauveau, S. Remy, P. J. Royer, M. Hill, S. Tanguy-Royer, F.-X. Hubert, L. Tesson, R. Brion, G. Beriou, M. Gregoire, et al. Heme oxygenase-1 expression inhibits dendritic cell maturation and proinflammatory function but conserves IL-10 expression Blood, September 1, 2005; 106(5): 1694 - 1702. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Nalbandian, V. Paharkova-Vatchkova, A. Mao, S. Nale, and S. Kovats The Selective Estrogen Receptor Modulators, Tamoxifen and Raloxifene, Impair Dendritic Cell Differentiation and Activation J. Immunol., August 15, 2005; 175(4): 2666 - 2675. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G.-Y. Kim, K.-H. Kim, S.-H. Lee, M.-S. Yoon, H.-J. Lee, D.-O. Moon, C.-M. Lee, S.-C. Ahn, Y. C. Park, and Y.-M. Park Curcumin Inhibits Immunostimulatory Function of Dendritic Cells: MAPKs and Translocation of NF-{kappa}B as Potential Targets J. Immunol., June 15, 2005; 174(12): 8116 - 8124. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J Grisar, M Aringer, M D Koller, G H Stummvoll, D Eselbock, B Zwolfer, C W Steiner, B Zierhut, L Wagner, P Pietschmann, et al. Leflunomide inhibits transendothelial migration of peripheral blood mononuclear cells Ann Rheum Dis, December 1, 2004; 63(12): 1632 - 1637. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. N. Georas Inhaled Glucocorticoids, Lymphocytes, and Dendritic Cells in Asthma and Obstructive Lung Diseases Proceedings of the ATS, November 1, 2004; 1(3): 215 - 221. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. A. Long, M. Fogel-Petrovic, D. A. Knight, P. J. Thompson, and J. W. Upham Higher Prostaglandin E2 Production by Dendritic Cells from Subjects with Asthma Compared with Normal Subjects Am. J. Respir. Crit. Care Med., September 1, 2004; 170(5): 485 - 491. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Monti, B. E. Leone, A. Zerbi, G. Balzano, S. Cainarca, V. Sordi, M. Pontillo, A. Mercalli, V. Di Carlo, P. Allavena, et al. Tumor-Derived MUC1 Mucins Interact with Differentiating Monocytes and Induce IL-10highIL-12low Regulatory Dendritic Cell J. Immunol., June 15, 2004; 172(12): 7341 - 7349. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. E. Wiley, M. Cwiartka, D. Alvarez, D. C. Mackenzie, J. R. Johnson, S. Goncharova, L. Lundblad, and M. Jordana Transient Corticosteroid Treatment Permanently Amplifies the Th2 Response in a Murine Model of Asthma J. Immunol., April 15, 2004; 172(8): 4995 - 5005. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. Mirenda, I. Berton, J. Read, T. Cook, J. Smith, A. Dorling, and R. I. Lechler Modified Dendritic Cells Coexpressing Self and Allogeneic Major Histocompatability Complex Molecules: An Efficient Way to Induce Indirect Pathway Regulation J. Am. Soc. Nephrol., April 1, 2004; 15(4): 987 - 997. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. Banki, L. Kacani, B. Mullauer, D. Wilflingseder, G. Obermoser, H. Niederegger, H. Schennach, G. M. Sprinzl, N. Sepp, A. Erdei, et al. Cross-Linking of CD32 Induces Maturation of Human Monocyte-Derived Dendritic Cells Via NF-{kappa}B Signaling Pathway J. Immunol., April 15, 2003; 170(8): 3963 - 3970. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J Plumb, M A Armstrong, M Duddy, M Mirakhur, and S McQuaid CD83-positive dendritic cells are present in occasional perivascular cuffs in multiple sclerosis lesions Multiple Sclerosis, April 1, 2003; 9(2): 142 - 147. [Abstract] [PDF]
|
|
|
|
|
|
A. M. Woltman and C. van Kooten Functional modulation of dendritic cells to suppress adaptive immune responses J. Leukoc. Biol., April 1, 2003; 73(4): 428 - 441. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 U. Kammerer, A. O. Eggert, M. Kapp, A. D. McLellan, T. B. H. Geijtenbeek, J. Dietl, Y. van Kooyk, and E. Kampgen Unique Appearance of Proliferating Antigen-Presenting Cells Expressing DC-SIGN (CD209) in the Decidua of Early Human Pregnancy Am. J. Pathol., March 1, 2003; 162(3): 887 - 896. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Bayry, S. Lacroix-Desmazes, C. Carbonneil, N. Misra, V. Donkova, A. Pashov, A. Chevailler, L. Mouthon, B. Weill, P. Bruneval, et al. Inhibition of maturation and function of dendritic cells by intravenous immunoglobulin Blood, January 15, 2003; 101(2): 758 - 765. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Duperrier, A. Farre, J. Bienvenu, N. Bleyzac, J. Bernaud, L. Gebuhrer, D. Rigal, and A. Eljaafari Cyclosporin A inhibits dendritic cell maturation promoted by TNF-{alpha} or LPS but not by double-stranded RNA or CD40L J. Leukoc. Biol., November 1, 2002; 72(5): 953 - 961. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Hackstein, T. Taner, A. J. Logar, and A. W. Thomson Rapamycin inhibits macropinocytosis and mannose receptor-mediated endocytosis by bone marrow-derived dendritic cells Blood, July 18, 2002; 100(3): 1084 - 1087. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. M. Woltman, C. Massacrier, J. W. de Fijter, C. Caux, and C. van Kooten Corticosteroids Prevent Generation of CD34+-Derived Dermal Dendritic Cells But Do Not Inhibit Langerhans Cell Development J. Immunol., June 15, 2002; 168(12): 6181 - 6188. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Relloso, A. Puig-Kroger, O. M. Pello, J. L. Rodriguez-Fernandez, G. de la Rosa, N. Longo, J. Navarro, M. A. Munoz-Fernandez, P. Sanchez-Mateos, and A. L. Corbi DC-SIGN (CD209) Expression Is IL-4 Dependent and Is Negatively Regulated by IFN, TGF-{beta}, and Anti-Inflammatory Agents J. Immunol., March 15, 2002; 168(6): 2634 - 2643. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. D. Saemann, O. Parolini, G. A. Bohmig, P. Kelemen, P.-M. Krieger, J. Neumuller, K. Knarr, W. Kammlander, W. H. Horl, C. Diakos, et al. Bacterial metabolite interference with maturation of human monocyte-derived dendritic cells J. Leukoc. Biol., February 1, 2002; 71(2): 238 - 246. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Hackstein, A. E. Morelli, A. T. Larregina, R. W. Ganster, G. D. Papworth, A. J. Logar, S. C. Watkins, L. D. Falo, and A. W. Thomson Aspirin Inhibits In Vitro Maturation and In Vivo Immunostimulatory Function of Murine Myeloid Dendritic Cells J. Immunol., June 15, 2001; 166(12): 7053 - 7062. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Corinti, C. Albanesi, A. la Sala, S. Pastore, and G. Girolomoni Regulatory Activity of Autocrine IL-10 on Dendritic Cell Functions J. Immunol., April 1, 2001; 166(7): 4312 - 4318. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. D. Kim, Y.-K. Choe, I. S. Choe, and J.-S. Lim Inhibition of glucocorticoid-mediated, caspase-independent dendritic cell death by CD40 activation J. Leukoc. Biol., March 1, 2001; 69(3): 426 - 434. [Abstract] [Full Text]
|
|
|
|
 |
|
 B. Knight, G. C.T. Yeoh, K. L. Husk, T. Ly, L. J. Abraham, C. Yu, J. A. Rhim, and N. Fausto Impaired Preneoplastic Changes and Liver Tumor Formation in Tumor Necrosis Factor Receptor Type 1 Knockout Mice J. Exp. Med., December 18, 2000; 192(12): 1809 - 1818. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Shimizu, S.-i. Fujii, K. Fujimoto, K. Kawa, A. Yamada, and F. Kawano Tacrolimus (FK506) treatment of CD34+ hematopoietic progenitor cells promote the development of dendritic cells that drive CD4+ T cells toward Th2 responses J. Leukoc. Biol., November 1, 2000; 68(5): 633 - 640. [Abstract] [Full Text]
|
|
|
|
|
|
D. Rea, C. van Kooten, K. E. van Meijgaarden, T. H. M. Ottenhoff, C. J. M. Melief, and R. Offringa Glucocorticoids transform CD40-triggering of dendritic cells into an alternative activation pathway resulting in antigen-presenting cells that secrete IL-10 Blood, May 15, 2000; 95(10): 3162 - 3167. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Piemonti, P. Monti, M. Sironi, P. Fraticelli, B. E. Leone, E. Dal Cin, P. Allavena, and V. Di Carlo Vitamin D3 Affects Differentiation, Maturation, and Function of Human Monocyte-Derived Dendritic Cells J. Immunol., May 1, 2000; 164(9): 4443 - 4451. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Komi and O. Lassila Nonsteroidal anti-estrogens inhibit the functional differentiation of human monocyte-derived dendritic cells Blood, May 1, 2000; 95(9): 2875 - 2882. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Penna and L. Adorini 1{alpha},25-Dihydroxyvitamin D3 Inhibits Differentiation, Maturation, Activation, and Survival of Dendritic Cells Leading to Impaired Alloreactive T Cell Activation J. Immunol., March 1, 2000; 164(5): 2405 - 2411. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. E. Demeure, H. Tanaka, V. Mateo, M. Rubio, G. Delespesse, and M. Sarfati CD47 Engagement Inhibits Cytokine Production and Maturation of Human Dendritic Cells J. Immunol., February 15, 2000; 164(4): 2193 - 2199. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Piemonti, P. Monti, P. Allavena, B. E. Leone, A. Caputo, and V. Di Carlo Glucocorticoids increase the endocytic activity of human dendritic cells Int. Immunol., September 1, 1999; 11(9): 1519 - 1526. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
