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Antigen Secreted from Noncytosolic Listeria monocytogenes Is Processed by the Classical MHC Class I Processing Pathway¹

^* Division of Basic Immunology, Department of Medicine, National Jewish Medical and Research Center, Denver, CO 80206; and Department of Immunology and the Cancer Center, University of Colorado Health Sciences Center, Denver, CO 80262

Intracellular bacteria can reside in a vacuolar compartment, or they can escape the vacuole and become free living in the cytoplasm. The presentation of Ag by class I MHC molecules has been defined primarily for Ag present in the cytoplasm. It was therefore thought that Ags from bacteria that remain in a vacuole would not be presented by MHC class I molecules. Although some studies have provided data to support this idea, it is not necessarily true for all intracellular bacteria. For example, we have previously demonstrated that an epitope from the p60 protein secreted by LLO^- Listeria monocytogenes, which does not reside in the cytoplasm, can be presented by MHC class I molecules to a T cell clone specific for the epitope, p60_217–225. We have further examined the route by which Ag secreted by LLO^- L. monocytogenes is presented by MHC class I molecules. Using pharmacological inhibitors, we demonstrate that MHC class I presentation of the p60 epitope derived from by LLO^- L. monocytogenes requires phagolysosome fusion and processing by the proteasome. Lysosomal cathepsins, however, are not required for processing of the p60 epitope. Similarly, processing of the AttM epitope, secreted by LLO^- L. monocytogenes and presented by H2-M3, also requires phagolysosome fusion and cleavage by the proteasome. Thus, p60 and AttM secreted by LLO^- L. monocytogenes are processed via the classical class I pathway for presentation by MHC class I molecules.

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