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Department of Physiology and Biophysics, University of Calgary, Calgary, Alberta, Canada
Selective recruitment of eosinophils to sites of allergic and
parasitic inflammation involves specific adhesion and activation
signals expressed on or presented by stimulated endothelial cells. Here
we examined leukocyte recruitment on cytokine-activated HUVEC under
flow conditions. We perfused whole blood through a flow chamber to
examine mechanisms of selective leukocyte recruitment. Although there
was substantial recruitment of leukocytes on TNF-
-stimulated HUVEC,
we found no selective accumulation of any particular leukocyte
subpopulations. In contrast, fewer leukocytes were recruited to
IL-4-stimulated HUVEC, but the recruitment was selective for
eosinophils. We examined the role of adhesion molecules in these
interactions and found that eosinophil recruitment was completely
blocked with an 4 integrin mAb at the shear rates
examined. A significant number of neutrophils were also recruited to
IL-4-stimulated HUVEC, and these interactions required P-selectin and
P-selectin glycoprotein ligand-1. Thus, whole blood perfusion over
cytokine-activated endothelium revealed that IL-4-stimulated HUVEC
support selective recruitment of eosinophils, whereas
TNF--stimulated HUVEC lack selectivity for any leukocyte
subclass.
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