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Department of Immunology, Medical Sciences Building, University of Toronto, Toronto, Canada
Many CTL epitopes of clinical importance, particularly those derived from tumor Ags, display relatively poor MHC binding affinity and stability. Because in vivo immunogenicity, and thus the efficacy of peptide-based vaccines, is thought to be determined by MHC/peptide complex stability, there is a need to develop a simple strategy for enhancing the binding of suboptimal epitopes. Toward this goal, the ability to enhance suboptimal peptides through covalent linkage to ß2-microglobulin (ß2m) was explored. Two suboptimal variants of a high-affinity Db-restricted influenza nucleoprotein peptide were covalently linked, via a polypeptide spacer, to the amino terminus of human ß2m and the recombinant fusion proteins expressed in Escherichia coli. When compared with their uncoupled counterparts, the ß2m-linked epitopes display enhanced MHC stabilization and antigenicity. Thus, tethering epitopes to ß2m provides a simple method for augmenting the biological activity of suboptimal peptides and could be useful in the design of peptide-based vaccines or immunotherapeutics.
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