| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Departments of
*
Pediatrics and
Internal Medicine and
Sealy Center for Molecular Sciences, University of Texas Medical Branch, Galveston, TX 77555
One of several routes of achieving immunologic tolerance is through
functional inactivation of Ag-specific T cells. Oral administration of
Ag can allow survival of the Ag-specific T cells that are functionally
anergic. The aim of this study was to investigate whether functional
inactivation of Ag-specific T cells is directed through an activation
process and to further define the differentiative pathways and
functional characteristics of anergic T cells. Mice were transplanted
with OVA-specific TCR-transgenic T cells and either fed OVA or
immunized s.c. with the OVA peptide 323–339 in CFA. OVA-specific T
cells from OVA-fed mice were unresponsive to restimulation in vitro
within 48–72 h after treatment. In vivo, however, T cell proliferation
was detected by 5,6-carboxy-succinimidyl-fluoresceine-ester intensity
changes in OVA-specific T cells. The mesenteric lymph nodes (LNs) from
OVA-fed mice more frequently contained OVA-specific dividing cells in
vivo than those in the peripheral LNs, and the reciprocal was observed
following s.c. immunization of the OVA peptide in CFA. The induction of
anergy in OVA-fed mice was accompanied by rapid up-regulation of CD69
and CTLA-4, later down-regulation of CD45RB on OVA-specific T cells,
and a marked decrease in T cell secretion of IL-2, IL-10, and IFN-
after OVA restimulation in vitro. Results from this study indicate that
the inductive phase of oral tolerance is preceded by Ag-specific T cell
activation in vivo, proliferation in the regional draining LNs, and
differentiation into a memory-like state. These results indicate that
Ag-directed differentiation occurs as a part of T cell tolerance
through anergy.
This article has been cited by other articles:
|
|
 |
|
  J. Sun, B. Tumurbaatar, J. Jia, H. Diao, F. Bodola, S. M. Lemon, W. Tang, D. G. Bowen, G. W. McCaughan, P. Bertolino, et al. Parenchymal Expression of CD86/B7.2 Contributes to Hepatitis C Virus-Related Liver Injury J. Virol., August 15, 2005; 79(16): 10730 - 10739. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Porporatto, I. D. Bianco, and S. G. Correa Local and systemic activity of the polysaccharide chitosan at lymphoid tissues after oral administration J. Leukoc. Biol., July 1, 2005; 78(1): 62 - 69. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. H. Zinselmeyer, J. Dempster, A. M. Gurney, D. Wokosin, M. Miller, H. Ho, O. R. Millington, K. M. Smith, C. M. Rush, I. Parker, et al. In situ characterization of CD4+ T cell behavior in mucosal and systemic lymphoid tissues during the induction of oral priming and tolerance J. Exp. Med., June 6, 2005; 201(11): 1815 - 1823. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. Z. Mehal, S. Z. Sheikh, L. Gorelik, and R. A. Flavell TGF-{beta} signaling regulates CD8+ T cell responses to high- and low-affinity TCR interactions Int. Immunol., May 1, 2005; 17(5): 531 - 538. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. W. Smith and C. Nagler-Anderson Preventing Intolerance: The Induction of Nonresponsiveness to Dietary and Microbial Antigens in the Intestinal Mucosa J. Immunol., April 1, 2005; 174(7): 3851 - 3857. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. R. Millington, A. McI. Mowat, and P. Garside Induction of Bystander Suppression by Feeding Antigen Occurs despite Normal Clonal Expansion of the Bystander T Cell Population J. Immunol., November 15, 2004; 173(10): 6059 - 6064. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Porporatto, I. D. Bianco, A. M. Cabanillas, and S. G. Correa Early events associated to the oral co-administration of type II collagen and chitosan: induction of anti-inflammatory cytokines Int. Immunol., March 1, 2004; 16(3): 433 - 441. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. T. Tobagus, W. R. Thomas, and P. G. Holt Adjuvant Costimulation during Secondary Antigen Challenge Directs Qualitative Aspects of Oral Tolerance Induction, Particularly during the Neonatal Period J. Immunol., February 15, 2004; 172(4): 2274 - 2285. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. M. Tiemessen, S. Kunzmann, C. B. Schmidt-Weber, J. Garssen, C. A. F. M. Bruijnzeel-Koomen, E. F. Knol, and E. Van Hoffen Transforming growth factor-{beta} inhibits human antigen-specific CD4+ T cell proliferation without modulating the cytokine response Int. Immunol., December 1, 2003; 15(12): 1495 - 1504. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. W. J. Unger, F. Hauet-Broere, W. Jansen, L. A. van Berkel, G. Kraal, and J. N. Samsom Early Events in Peripheral Regulatory T Cell Induction via the Nasal Mucosa J. Immunol., November 1, 2003; 171(9): 4592 - 4603. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Dubois, L. Chapat, A. Goubier, M. Papiernik, J.-F. Nicolas, and D. Kaiserlian Innate CD4+CD25+ regulatory T cells are required for oral tolerance and inhibition of CD8+ T cells mediating skin inflammation Blood, November 1, 2003; 102(9): 3295 - 3301. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Beacock-Sharp, A. M. Donachie, N. C. Robson, and A. M. Mowat A role for dendritic cells in the priming of antigen-specific CD4+ and CD8+ T lymphocytes by immune-stimulating complexes in vivo Int. Immunol., June 1, 2003; 15(6): 711 - 720. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. C. Nathwani, A. M. Davidoff, H. Hanawa, Y. Hu, F. A. Hoffer, A. Nikanorov, C. Slaughter, C. Y. C. Ng, J. Zhou, J. N. Lozier, et al. Sustained high-level expression of human factor IX (hFIX) after liver-targeted delivery of recombinant adeno-associated virus encoding the hFIX gene in rhesus macaques Blood, August 13, 2002; 100(5): 1662 - 1669. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. C. Tsitoura, V. P. Yeung, R. H. DeKruyff, and D. T. Umetsu Critical role of B cells in the development of T cell tolerance to aeroallergens Int. Immunol., June 1, 2002; 14(6): 659 - 667. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Baron, I. McMorrow, D. H Sachs, and C. LeGuern Persistence of Dominant T Cell Clones in Accepted Solid Organ Transplants J. Immunol., October 15, 2001; 167(8): 4154 - 4160. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. George-Chandy, K. Eriksson, M. Lebens, I. Nordstrom, E. Schon, and J. Holmgren Cholera Toxin B Subunit as a Carrier Molecule Promotes Antigen Presentation and Increases CD40 and CD86 Expression on Antigen-Presenting Cells Infect. Immun., September 1, 2001; 69(9): 5716 - 5725. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. L. Meyer, J. Benson, F. Song, N. Javed, I. E. Gienapp, J. Goverman, T. A. Brabb, L. Hood, and C. C. Whitacre Rapid Depletion of Peripheral Antigen-Specific T Cells in TCR-Transgenic Mice After Oral Administration of Myelin Basic Protein J. Immunol., May 1, 2001; 166(9): 5773 - 5781. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. M. Godsel, K. Wang, B. A. Schodin, J. S. Leon, S. D. Miller, and D. M. Engman Prevention of Autoimmune Myocarditis Through the Induction of Antigen-Specific Peripheral Immune Tolerance Circulation, March 27, 2001; 103(12): 1709 - 1714. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. N. Shi, H. Y. Liu, and C. Nagler-Anderson Enteric Infection Acts as an Adjuvant for the Response to a Model Food Antigen J. Immunol., December 1, 2000; 165(11): 6174 - 6182. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Hayday and J. L. Viney The Ins and Outs of Body Surface Immunology Science, October 6, 2000; 290(5489): 97 - 100. [Abstract] [Full Text]
|
|
|
|
 |
|
 K. M. SMITH, A. D. EATON, L. M. FINLAYSON, and P. GARSIDE Oral Tolerance Am. J. Respir. Crit. Care Med., October 1, 2000; 162(4): S175 - 178. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Blanas, G. M. Davey, F. R. Carbone, and W. R. Heath A Bone Marrow-Derived APC in the Gut-Associated Lymphoid Tissue Captures Oral Antigens and Presents Them to Both CD4+ and CD8+ T Cells J. Immunol., March 15, 2000; 164(6): 2890 - 2896. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. A. Frauwirth, M.-L. Alegre, and C. B. Thompson Induction of T Cell Anergy in the Absence of CTLA-4/B7 Interaction J. Immunol., March 15, 2000; 164(6): 2987 - 2993. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Desvignes, N. Etchart, J. Kehren, I. Akiba, J.-F. Nicolas, and D. Kaiserlian Oral Administration of Hapten Inhibits In Vivo Induction of Specific Cytotoxic CD8+ T Cells Mediating Tissue Inflammation: A Role for Regulatory CD4+ T Cells J. Immunol., March 1, 2000; 164(5): 2515 - 2522. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
