The JI
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     
 

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Perry, D. G.
Right arrow Articles by Martin, W. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Perry, D. G.
Right arrow Articles by Martin, W. J., II
The Journal of Immunology, 1999, 162: 380-386.
Copyright © 1999 by The American Association of Immunologists

Clathrin-Coated Pit-Associated Proteins Are Required for Alveolar Macrophage Phagocytosis1

Douglas G. Perry2, Gena L. Daugherty and William J. Martin, II

Division of Pulmonary and Critical Care Medicine, Indiana University School of Medicine, Indianapolis, IN 46202

During phagocytosis, phagocytic receptors and membrane material must be inserted in the pseudopod membrane as it extends over the phagocytic target. This may require a clathrin-mediated recycling mechanism similar to that postulated for leading edge formation during cell migration. To investigate this possibility, liposomes were used to deliver to intact rat alveolar macrophages (AMs): 1) Abs to clathrin, clathrin adaptor AP-2, and hsc70, and 2) amantadine. Phagocytosis was assayed by fluorometric and colorimetric techniques. Liposome-delivered Abs to clathrin and AP-2 inhibited AM phagocytosis of zymosan-coated, fluorescent liposomes from 16.3 ± 0.3 to 5.8 ± 0.3, and 10.1 ± 0.9 to 4.8 ± 0.2 liposomes/cell (p < 0.01). Similarly, liposome-delivered Ab to clathrin also inhibited AM phagocytosis of IgG-opsonized RBCs from 11.7 ± 1.7 to 3.8 ± 0.7 RBCs/cell (p < 0.01). Amantadine, which blocks the budding of clathrin-coated vesicles, inhibited phagocytosis from 13.8 ± 0.8 to 5.7 ± 0.6 (p < 0.01). Ab blockade of hsc70, which catalyzes clathrin turnover, also inhibited phagocytosis from 9.1 ± 0.5 to 4.3 ± 0.2 (p < 0.01). These findings suggest that clathrin-mediated receptor/membrane recycling is required for phagocytosis.




This article has been cited by other articles:


Home page
 Mol. Biol. CellHome page
V. Braun, C. Deschamps, G. Raposo, P. Benaroch, A. Benmerah, P. Chavrier, and F. Niedergang
AP-1 and ARF1 Control Endosomal Dynamics at Sites of FcR mediated Phagocytosis
Mol. Biol. Cell, December 1, 2007; 18(12): 4921 - 4931.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
G. Vidarsson, A. M. Stemerding, N. M. Stapleton, S. E. Spliethoff, H. Janssen, F. E. Rebers, M. de Haas, and J. G. van de Winkel
FcRn: an IgG receptor on phagocytes with a novel role in phagocytosis
Blood, November 15, 2006; 108(10): 3573 - 3579.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
O. Meier, M. Gastaldelli, K. Boucke, S. Hemmi, and U. F. Greber
Early Steps of Clathrin-Mediated Endocytosis Involved in Phagosomal Escape of Fc{gamma} Receptor-Targeted Adenovirus
J. Virol., February 15, 2005; 79(4): 2604 - 2613.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. Hashimoto, A. Hashimoto, A. Yamada, C. Kojima, H. Yamamoto, T. Tsutsumi, M. Higashi, A. Mizoguchi, R. Yagi, and H. Sabe
A Novel Mode of Action of an ArfGAP, AMAP2/PAG3/Pap{alpha}, in Arf6 Function
J. Biol. Chem., September 3, 2004; 279(36): 37677 - 37684.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
Y. Lefkir, M. Malbouyres, D. Gotthardt, A. Ozinsky, S. Cornillon, F. Bruckert, A. A. Aderem, T. Soldati, P. Cosson, and F. Letourneur
Involvement of the AP-1 Adaptor Complex in Early Steps of Phagocytosis and Macropinocytosis
Mol. Biol. Cell, February 1, 2004; 15(2): 861 - 869.
[Abstract] [Full Text] [PDF]

Home page
 J BiochemHome page
H. Sabe
Requirement for Arf6 in Cell Adhesion, Migration, and Cancer Cell Invasion
J. Biochem., October 1, 2003; 134(4): 485 - 489.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
N. Vanderheijden, P. L. Delputte, H. W. Favoreel, J. Vandekerckhove, J. Van Damme, P. A. van Woensel, and H. J. Nauwynck
Involvement of Sialoadhesin in Entry of Porcine Reproductive and Respiratory Syndrome Virus into Porcine Alveolar Macrophages
J. Virol., August 1, 2003; 77(15): 8207 - 8215.
[Abstract] [Full Text] [PDF]

Home page
 JCBHome page
F. Niedergang, E. Colucci-Guyon, T. Dubois, G. Raposo, and P. Chavrier
ADP ribosylation factor 6 is activated and controls membrane delivery during phagocytosis in macrophages
J. Cell Biol., June 23, 2003; 161(6): 1143 - 1150.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. M. L. Tse, W. Furuya, E. Gold, A. D. Schreiber, K. Sandvig, R. D. Inman, and S. Grinstein
Differential Role of Actin, Clathrin, and Dynamin in Fcgamma Receptor-mediated Endocytosis and Phagocytosis
J. Biol. Chem., January 24, 2003; 278(5): 3331 - 3338.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Website Copyright © 1999 by The American Association of Immunologists, Inc. All rights reserved.
All Contents Copyright © 1999 by The American Association of Immunologists, Inc. All rights reserved.