| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
*
Institute of Immunology, University of Vienna, Vienna Austria;
Division of Endocrinology and
Division of Nephrology and Dialysis, Department of Internal Medicine III,
§
Institute of General and Experimental Pathology, University of Vienna, Vienna, Austria; and
¶
Novartis Research Institute, Vienna, Austria
We have assessed the functional effect of CD99 engagement on
resting human peripheral blood (PB) T cells. CD99, as detected by the
mAb 3B2/TA8, is constitutively expressed on all PB T cells and becomes
further up-regulated upon cellular activation. In this study we
demonstrate that cross-linking of the CD99 molecule with the agonistic
mAb 3B2/TA8 cooperates with suboptimal TCR/CD3 signals, but not with
phorbol ester, ionomycin, or CD28 mAb stimulation, to induce
proliferation of resting PB T cells. Comparable stimulatory effects
were observed with the CD99 mAb 12E7. Characterization of the signaling
pathways involved revealed that CD99 engagement leads to the elevation
of intracellular Ca2+, which is dependent on the cell
surface expression of the TCR/CD3 complex. No CD99 mAb-induced calcium
mobilization was observed on TCR/CD3-modulated or TCR/CD3-negative T
cells. To examine the impact of CD99 stimulation on subsequent cytokine
production by T cells, we cross-linked CD99 molecules in the presence
of a suboptimal TCR/CD3 trigger followed by determination of
intracellular cytokine levels. Significantly, T cell lines as well as
Th1 and Th0 clones synthesized TNF-
and IFN-
after this
treatment. In contrast, Th2 clones were unable to produce IL-4 or
IFN- when stimulated in a similar fashion. We conclude that CD99 is
a receptor that mediates TCR/CD3-dependent activation of resting PB T
cells and specifically induces Th1-type cytokine production in
polyclonally activated T cell lines, Th1 and Th0
clones.
This article has been cited by other articles:
|
|
 |
|
  E. J.A. van Wanrooij, P. de Vos, M. G. Bixel, D. Vestweber, T. J.C. van Berkel, and J. Kuiper Vaccination against CD99 inhibits atherogenesis in low-density lipoprotein receptor-deficient mice Cardiovasc Res, June 1, 2008; 78(3): 590 - 596. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H.-J. Byun, I.-K. Hong, E. Kim, Y.-J. Jin, D.-I. Jeoung, J.-H. Hahn, Y.-M. Kim, S. H. Park, and H. Lee A Splice Variant of CD99 Increases Motility and MMP-9 Expression of Human Breast Cancer Cells through the AKT-, ERK-, and JNK-dependent AP-1 Activation Signaling Pathways J. Biol. Chem., November 17, 2006; 281(46): 34833 - 34847. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A.-M. Imbert, G. Belaaloui, F. Bardin, C. Tonnelle, M. Lopez, and C. Chabannon CD99 expressed on human mobilized peripheral blood CD34+ cells is involved in transendothelial migration Blood, October 15, 2006; 108(8): 2578 - 2586. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. L. W. Yun, A. A. DeCarlo, and N. Hunter Gingipains of Porphyromonas gingivalis Modulate Leukocyte Adhesion Molecule Expression Induced in Human Endothelial Cells by Ligation of CD99 Infect. Immun., March 1, 2006; 74(3): 1661 - 1672. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Bixel, S. Kloep, S. Butz, B. Petri, B. Engelhardt, and D. Vestweber Mouse CD99 participates in T-cell recruitment into inflamed skin Blood, November 15, 2004; 104(10): 3205 - 3213. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Diakos, E. E. Prieschl, M. Saemann, V. Novotny, G. Bohmig, R. Csonga, T. Baumruker, and G. J. Zlabinger Novel Mode of Interference with Nuclear Factor of Activated T-cells Regulation in T-cells by the Bacterial Metabolite n-Butyrate J. Biol. Chem., June 28, 2002; 277(27): 24243 - 24251. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Stockl, O. Majdic, G. Fischer, D. Maurer, and W. Knapp Monomorphic Molecules Function as Additional Recognition Structures on Haptenated Target Cells for HLA-A1-Restricted, Hapten-Specific CTL J. Immunol., September 1, 2001; 167(5): 2724 - 2733. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I.-s. Lee, M. K. Kim, E. Y. Choi, A. Mehl, K. C. Jung, M. C. Gil, M. Rowe, and S. H. Park CD99 expression is positively regulated by Sp1 and is negatively regulated by Epstein-Barr virus latent membrane protein 1 through nuclear factor-{kappa}B Blood, June 1, 2001; 97(11): 3596 - 3604. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. D. Pettersen, G. Bernard, M. K. Olafsen, M. Pourtein, and S. O. Lie CD99 Signals Caspase-Independent T Cell Death J. Immunol., April 15, 2001; 166(8): 4931 - 4942. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Scotlandi, N. Baldini, V. Cerisano, M. C. Manara, S. Benini, M. Serra, P.-L. Lollini, P. Nanni, G. Nicoletti, G. Bernard, et al. CD99 Engagement: An Effective Therapeutic Strategy for Ewing Tumors Cancer Res., September 1, 2000; 60(18): 5134 - 5142. [Abstract] [Full Text]
|
|
|
|
|
|
E. Riedl, J. Stockl, O. Majdic, C. Scheinecker, K. Rappersberger, W. Knapp, and H. Strobl Functional Involvement of E-Cadherin in TGF-{beta}1-Induced Cell Cluster Formation of In Vitro Developing Human Langerhans-Type Dendritic Cells J. Immunol., August 1, 2000; 165(3): 1381 - 1386. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
