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Cutting Edge: Senescent BALB/c Mice Exhibit Decreased Expression of 5 Surrogate Light Chains and Reduced Development Within the Pre-B Cell Compartment¹

^* Department of Microbiology and Immunology, University of Miami School of Medicine, Miami, FL 33101

Although senescent BALB/c mice (2 years old) have reduced numbers of small pre-B cells, early pre-B cells (CD43⁺CD25⁺B220⁺) are present in comparable numbers within the bone marrow of both young (3–6-month-old) and senescent BALB/c mice. The transition of CD43⁺ pre-B cells to the CD43^- pre-B cell compartments is dependent on proliferation and clonal maturation dictated by the pre-B cell receptor (µ/5/VpreB). In vivo, senescent CD43⁺B220⁺ pro-B/early pre-B cells demonstrated reduction of 5 mRNA, by RT-PCR analysis, and of both surface and cytoplasmic 5 protein. Decreased 5 protein expression was also seen among pro-B/pre-B cells derived from senescent bone marrow after stimulation in vitro with IL-7. We propose that diminished expression of the 5 surrogate light chain results in decreased pre-B cell receptor formation and contributes to reduced recruitment of nascent CD43⁺ pre-B cells into the CD43^- large and small pre-B cell compartments.