| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
CUTTING EDGE |
Department of Internal Medicine, Pulmonary and Critical Care Section, Yale University School of Medicine, New Haven, CT 06520
The cytokines IL-4 and IL-5 are often coordinately produced by Th2 cells as in asthma. However, it is unclear whether similar molecular mechanisms underlie transcription of the two genes. We have previously shown that the transcription factor GATA-3 is expressed in Th2 but not Th1 cells and is crucial for activation of the IL-5 promoter by different stimuli. In a different study, GATA-3 was shown to be sufficient for the expression of IL-4 and other Th2 cytokine genes. Here, we show that ectopic expression of GATA-3 is sufficient to drive IL-5 but not IL-4 gene expression. Also, in Th2 cells, antisense GATA-3 RNA inhibits IL-5 but not IL-4 promoter activation. The induction of IL-5 gene expression by GATA-3 involves high affinity binding of GATA-3 to an inverted GATA repeat in the IL-5 promoter.
This article has been cited by other articles:
|
|
 |
|
  X. Zhao, B. Zheng, Y. Huang, D. Yang, S. Katzman, C. Chang, D. Fowell, and W.-p. Zeng Interaction between GATA-3 and the Transcriptional Coregulator Pias1 Is Important for the Regulation of Th2 Immune Responses J. Immunol., December 15, 2007; 179(12): 8297 - 8304. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Matsuno, Y. Ishii, K. Yoh, Y. Morishima, N. Haraguchi, N. Kikuchi, T. Iizuka, T. Kiwamoto, S. Homma, A. Nomura, et al. Overexpression of GATA-3 Protects against the Development of Hypersensitivity Pneumonitis Am. J. Respir. Crit. Care Med., November 15, 2007; 176(10): 1015 - 1025. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Yoshimoto, T. Yoshimoto, K. Yasuda, J. Mizuguchi, and K. Nakanishi IL-27 Suppresses Th2 Cell Development and Th2 Cytokines Production from Polarized Th2 Cells: A Novel Therapeutic Way for Th2-Mediated Allergic Inflammation J. Immunol., October 1, 2007; 179(7): 4415 - 4423. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Shinnakasu, M. Yamashita, K. Shinoda, Y. Endo, H. Hosokawa, A. Hasegawa, S. Ikemizu, and T. Nakayama Critical YxKxHxxxRP Motif in the C-Terminal Region of GATA3 for Its DNA Binding and Function J. Immunol., November 1, 2006; 177(9): 5801 - 5810. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Kimura, Y. Ishii, K. Yoh, Y. Morishima, T. Iizuka, T. Kiwamoto, Y. Matsuno, S. Homma, A. Nomura, T. Sakamoto, et al. Overexpression of the Transcription Factor GATA-3 Enhances the Development of Pulmonary Fibrosis Am. J. Pathol., July 1, 2006; 169(1): 96 - 104. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Usui, J. C. Preiss, Y. Kanno, Z. J. Yao, J. H. Bream, J. J. O'Shea, and W. Strober T-bet regulates Th1 responses through essential effects on GATA-3 function rather than on IFNG gene acetylation and transcription J. Exp. Med., March 20, 2006; 203(3): 755 - 766. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Shoemaker, M. Saraiva, and A. O'Garra GATA-3 Directly Remodels the IL-10 Locus Independently of IL-4 in CD4+ T Cells J. Immunol., March 15, 2006; 176(6): 3470 - 3479. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. I. Nigo, M. Yamashita, K. Hirahara, R. Shinnakasu, M. Inami, M. Kimura, A. Hasegawa, Y. Kohno, and T. Nakayama Regulation of allergic airway inflammation through Toll-like receptor 4-mediated modification of mast cell function PNAS, February 14, 2006; 103(7): 2286 - 2291. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Wang, M. F. Shannon, and I. G. Young A role for Ets1, synergizing with AP-1 and GATA-3 in the regulation of IL-5 transcription in mouse Th2 lymphocytes Int. Immunol., February 1, 2006; 18(2): 313 - 323. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. N. Georas, J. Guo, U. De Fanis, and V. Casolaro T-helper cell type-2 regulation in allergic disease Eur. Respir. J., December 1, 2005; 26(6): 1119 - 1137. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y.-K. Jee, J. Gilmour, A. Kelly, H. Bowen, D. Richards, C. Soh, P. Smith, C. Hawrylowicz, D. Cousins, T. Lee, et al. Repression of Interleukin-5 Transcription by the Glucocorticoid Receptor Targets GATA3 Signaling and Involves Histone Deacetylase Recruitment J. Biol. Chem., June 17, 2005; 280(24): 23243 - 23250. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. F. Zhong, Y. Zhao, S. Sutton, A. Su, Y. Zhan, L. Zhu, C. Yan, T. Gallaher, P. B. Johnston, W. F. Anderson, et al. Gene expression profile of murine long-term reconstituting vs. short-term reconstituting hematopoietic stem cells PNAS, February 15, 2005; 102(7): 2448 - 2453. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. I. Marusina, D.-K. Kim, L. D. Lieto, F. Borrego, and J. E. Coligan GATA-3 Is an Important Transcription Factor for Regulating Human NKG2A Gene Expression J. Immunol., February 15, 2005; 174(4): 2152 - 2159. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. N. Georas Inhaled Glucocorticoids, Lymphocytes, and Dendritic Cells in Asthma and Obstructive Lung Diseases Proceedings of the ATS, November 1, 2004; 1(3): 215 - 221. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Yamashita, R. Shinnakasu, Y. Nigo, M. Kimura, A. Hasegawa, M. Taniguchi, and T. Nakayama Interleukin (IL)-4-independent Maintenance of Histone Modification of the IL-4 Gene Loci in Memory Th2 Cells J. Biol. Chem., September 17, 2004; 279(38): 39454 - 39464. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Inami, M. Yamashita, Y. Tenda, A. Hasegawa, M. Kimura, K. Hashimoto, N. Seki, M. Taniguchi, and T. Nakayama CD28 Costimulation Controls Histone Hyperacetylation of the Interleukin 5 Gene Locus in Developing Th2 Cells J. Biol. Chem., May 28, 2004; 279(22): 23123 - 23133. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. Wu, L. Rinaldi, K. A. Fortner, J. Q. Russell, J. Tschopp, C. Irvin, and R. C. Budd Cellular FLIP Long Form-Transgenic Mice Manifest a Th2 Cytokine Bias and Enhanced Allergic Airway Inflammation J. Immunol., April 15, 2004; 172(8): 4724 - 4732. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. C. Webb, K. I. Matthaei, Y. Cai, A. N. J. McKenzie, and P. S. Foster Polymorphisms in IL-4R{alpha} Correlate with Airways Hyperreactivity, Eosinophilia, and Ym Protein Expression in Allergic IL-13-/- Mice J. Immunol., January 15, 2004; 172(2): 1092 - 1098. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Tamauchi, M. Terashima, M. Ito, H. Maruyama, N. Ikewaki, M. Inoue, X. Gao, K. Hozumi, and S. Habu Evidence of GATA-3-dependent Th2 commitment during the in vivo immune response Int. Immunol., January 1, 2004; 16(1): 179 - 187. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Ghirlando and C. D. Trainor Determinants of GATA-1 Binding to DNA: THE ROLE OF NON-FINGER RESIDUES J. Biol. Chem., November 14, 2003; 278(46): 45620 - 45628. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. S. Sundrud, S. M. Grill, D. Ni, K. Nagata, S. S. Alkan, A. Subramaniam, and D. Unutmaz Genetic Reprogramming of Primary Human T Cells Reveals Functional Plasticity in Th Cell Differentiation J. Immunol., October 1, 2003; 171(7): 3542 - 3549. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Yoh, K. Shibuya, N. Morito, T. Nakano, K. Ishizaki, H. Shimohata, M. Nose, S. Izui, A. Shibuya, A. Koyama, et al. Transgenic Overexpression of GATA-3 in T Lymphocytes Improves Autoimmune Glomerulonephritis in Mice with a BXSB/MpJ-Yaa Genetic Background J. Am. Soc. Nephrol., October 1, 2003; 14(10): 2494 - 2502. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Taha, Q. Hamid, L. Cameron, and R. Olivenstein T Helper Type 2 Cytokine Receptors and Associated Transcription Factors GATA-3, c-MAF, and Signal Transducer and Activator of Transcription Factor-6 in Induced Sputum of Atopic Asthmatic Patients Chest, June 1, 2003; 123(6): 2074 - 2082. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Kusam, L. M. Toney, H. Sato, and A. L. Dent Inhibition of Th2 Differentiation and GATA-3 Expression by BCL-6 J. Immunol., March 1, 2003; 170(5): 2435 - 2441. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Okuda, I. Takahashi, J.-K. Kim, N. Ohta, K. Iwatani, H. Iijima, Y. Kai, H. Tamagawa, T. Hiroi, M.-N. Kweon, et al. Development of Colitis in Signal Transducers and Activators of Transcription 6-Deficient T-Cell Receptor {alpha}-Deficient Mice: A Potential Role of Signal Transducers and Activators of Transcription 6-Independent Interleukin-4 Signaling for the Generation of Th2-Biased Pathological CD4+{beta}{beta}T Cells Am. J. Pathol., January 1, 2003; 162(1): 263 - 271. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Takemoto, K.-i. Arai, and S. Miyatake Cutting Edge: The Differential Involvement of the N-Finger of GATA-3 in Chromatin Remodeling and Transactivation During Th2 Development J. Immunol., October 15, 2002; 169(8): 4103 - 4107. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Arima, H. Toyama, H. Ichii, S. Kojima, S. Okada, M. Hatano, G. Cheng, M. Kubo, T. Fukuda, and T. Tokuhisa A Putative Silencer Element in the IL-5 Gene Recognized by Bcl6 J. Immunol., July 15, 2002; 169(2): 829 - 836. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Lavenu-Bombled, C. D. Trainor, I. Makeh, P.-H. Romeo, and I. Max-Audit Interleukin-13 Gene Expression Is Regulated by GATA-3 in T Cells. ROLE OF A CRITICAL ASSOCIATION OF A GATA AND TWO GATG MOTIFS J. Biol. Chem., May 17, 2002; 277(21): 18313 - 18321. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. B. Stephensen, R. Rasooly, X. Jiang, M. A. Ceddia, C. T. Weaver, R. A. S. Chandraratna, and R. P. Bucy Vitamin A Enhances in Vitro Th2 Development Via Retinoid X Receptor Pathway J. Immunol., May 1, 2002; 168(9): 4495 - 4503. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Kurata, H.-J. Lee, T. McClanahan, R. L. Coffman, A. O'Garra, and N. Arai Friend of GATA Is Expressed in Naive Th Cells and Functions As a Repressor of GATA-3-Mediated Th2 Cell Development J. Immunol., May 1, 2002; 168(9): 4538 - 4545. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. J. Kieffer, J. M. Greally, I. Landres, S. Nag, Y. Nakajima, T. Kohwi-Shigematsu, and P. B. Kavathas Identification of a Candidate Regulatory Region in the Human CD8 Gene Complex by Colocalization of DNase I Hypersensitive Sites and Matrix Attachment Regions Which Bind SATB1 and GATA-3 J. Immunol., April 15, 2002; 168(8): 3915 - 3922. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. P. Justice, M. T. Borchers, J. J. Lee, W. H. Rowan, Y. Shibata, and M. R. Van Scott Ragweed-induced expression of GATA-3, IL-4, and IL-5 by eosinophils in the lungs of allergic C57BL/6J mice Am J Physiol Lung Cell Mol Physiol, February 1, 2002; 282(2): L302 - L309. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. L. Wurster, D. J. Withers, T. Uchida, M. F. White, and M. J. Grusby Stat6 and IRS-2 Cooperate in Interleukin 4 (IL-4)-Induced Proliferation and Differentiation but Are Dispensable for IL-4-Dependent Rescue from Apoptosis Mol. Cell. Biol., January 1, 2002; 22(1): 117 - 126. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Zhou, W. Ouyang, Q. Gong, S. G. Katz, J. M. White, S. H. Orkin, and K. M. Murphy Friend of GATA-1 Represses GATA-3-dependent Activity in CD4+ T Cells J. Exp. Med., November 12, 2001; 194(10): 1461 - 1471. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Boonstra, F. J. Barrat, C. Crain, V. L. Heath, H. F. J. Savelkoul, and A. O'Garra 1{alpha},25-Dihydroxyvitamin D3 Has a Direct Effect on Naive CD4+ T Cells to Enhance the Development of Th2 Cells J. Immunol., November 1, 2001; 167(9): 4974 - 4980. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Kishikawa, J. Sun, A. Choi, S.-C. Miaw, and I-C. Ho The Cell Type-Specific Expression of the Murine IL-13 Gene Is Regulated by GATA-3 J. Immunol., October 15, 2001; 167(8): 4414 - 4420. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Wensky, M. C. Garibaldi Marcondes, and J. J. Lafaille The Role of IFN-{gamma} in the Production of Th2 Subpopulations: Implications for Variable Th2-Mediated Pathologies in Autoimmunity J. Immunol., September 15, 2001; 167(6): 3074 - 3081. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. C. Nawijn, G. M. Dingjan, R. Ferreira, B. N. Lambrecht, A. Karis, F. Grosveld, H. Savelkoul, and R. W. Hendriks Enforced Expression of GATA-3 in Transgenic Mice Inhibits Th1 Differentiation and Induces the Formation of a T1/ST2-Expressing Th2-Committed T Cell Compartment In Vivo J. Immunol., July 15, 2001; 167(2): 724 - 732. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Kim, K. Uyemura, M. K. Van Dyke, A. J. Legaspi, T. H. Rea, K. Shuai, and R. L. Modlin A Role for IL-12 Receptor Expression and Signal Transduction in Host Defense in Leprosy J. Immunol., July 15, 2001; 167(2): 779 - 786. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.C. Kips, K.G. Tournoy, and R.A. Pauwels New anti-asthma therapies: suppression of the effect of interleukin (IL)-4 and IL-5 Eur. Respir. J., March 1, 2001; 17(3): 499 - 506. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. C. Keen, L. Sholl, M. Wills-Karp, and S. N. Georas Preferential Activation of Nuclear Factor of Activated T Cells c Correlates with Mouse Strain Susceptibility to Allergic Responses and Interleukin-4 Gene Expression Am. J. Respir. Cell Mol. Biol., January 1, 2001; 24(1): 58 - 65. [Abstract] [Full Text]
|
|
|
|
|
|
N. Takemoto, Y. Kamogawa, H. Jun Lee, H. Kurata, K.-i. Arai, A. O'Garra, N. Arai, and S. Miyatake Cutting Edge: Chromatin Remodeling at the IL-4/IL-13 Intergenic Regulatory Region for Th2-Specific Cytokine Gene Cluster J. Immunol., December 15, 2000; 165(12): 6687 - 6691. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C.-H. Chen, D.-H. Zhang, J. M. LaPorte, and A. Ray Cyclic AMP Activates p38 Mitogen-Activated Protein Kinase in Th2 Cells: Phosphorylation of GATA-3 and Stimulation of Th2 Cytokine Gene Expression J. Immunol., November 15, 2000; 165(10): 5597 - 5605. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. H. Glimcher and K. M. Murphy Lineage commitment in the immune system: the T helper lymphocyte grows up Genes & Dev., July 15, 2000; 14(14): 1693 - 1711. [Full Text]
|
|
|
|
|
|
H. J. Lee, N. Takemoto, H. Kurata, Y. Kamogawa, S. Miyatake, A. O'Garra, and N. Arai GATA-3 Induces T Helper Cell Type 2 (Th2) Cytokine Expression and Chromatin Remodeling in Committed Th1 Cells J. Exp. Med., July 3, 2000; 192(1): 105 - 116. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Y. Karulin, M. D. Hesse, M. Tary-Lehmann, and P. V. Lehmann Single-Cytokine-Producing CD4 Memory Cells Predominate in Type 1 and Type 2 Immunity J. Immunol., February 15, 2000; 164(4): 1862 - 1872. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Yang, L. Cohn, D.-H. Zhang, R. Homer, A. Ray, and P. Ray Essential Role of Nuclear Factor kappa B in the Induction of Eosinophilia in Allergic Airway Inflammation J. Exp. Med., November 2, 1998; 188(9): 1739 - 1750. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Guo, V. Casolaro, E. Seto, W.-M. Yang, C. Chang, M.-C. Seminario, J. Keen, and S. N. Georas Yin-Yang 1 Activates Interleukin-4 Gene Expression in T Cells J. Biol. Chem., December 21, 2001; 276(52): 48871 - 48878. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
