| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
*
Department of Internal Medicine and Physical Therapy, University of Tokyo, Hongo, Bunkyo-ku, Tokyo, Japan;
Division of Protein Metabolism, Institute for Protein Research, Osaka University, Yamadaoka, Suita, Osaka, Japan; and
Department of Oncology, Institute of Medical Science, University of Tokyo, Shirokanedai, Minato-ku, Tokyo, Japan
Although the requirement for c-Src in extracellular matrix
(ECM)-mediated fibroblast motility has been well established, the roles
of hemopoietic Src family protein tyrosine kinases in leukocyte
migration have not been fully elucidated. To address the issue, we
analyzed fibronectin (Fn)-mediated adhesion signaling in rat basophilic
leukemia (RBL) 2H3 cells overexpressing 1) Csk, 2) a membrane-anchored,
gain-of-function Csk (mCsk), and 3) a kinase-defective mCsk (mCsk(-)).
Parent RBL2H3 cells, expressing autoactivated c-kit,
readily adhered to Fn-coated surface, developed typical leukocyte
adhesion machinery (podosome), and migrated toward Fn without cytokine
priming, thus provided a simple experimental system to analyze
Fn-mediated outside-in signaling. While overexpression of Csk or the
Csk mutants did not significantly affect cell adhesion to the Fn
surface or 
5 integrin recruitment to the attachment
sites, Csk suppressed and mCsk almost abolished Fn-mediated tyrosine
phosphorylation of paxillin, filamentous actin assembly to podosomes,
and cell migration, but mCsk(-) did not. Coexpression of LynA devoid
of C-terminal negative regulatory tyrosine in mCsk cells successfully
restored Fn-mediated podosome formation and cell migration.
Coexpression of c-Src lacking the C-terminal tyrosine reconstructed
podosomes, but could not restore the cell migration regardless of its
expression level. Collectively, these observations provide evidence
that Src family protein tyrosine kinases are required, and that Lyn
could transmit sufficient signal for Fn-mediated cytoskeletal changes
leading to cell locomotion in RBL2H3 cells, and they suggest that Lyn
and c-Src are differentially involved in cell
motility.
This article has been cited by other articles:
|
|
 |
|
  S. Kannan, A. Audet, H. Huang, L.-j. Chen, and M. Wu Cholesterol-Rich Membrane Rafts and Lyn Are Involved in Phagocytosis during Pseudomonas aeruginosa Infection J. Immunol., February 15, 2008; 180(4): 2396 - 2408. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J M W Gee, V E Shaw, S E Hiscox, R A McClelland, N K Rushmere, and R I Nicholson Deciphering antihormone-induced compensatory mechanisms in breast cancer and their therapeutic implications Endocr. Relat. Cancer, December 1, 2006; 13(Supplement_1): S77 - S88. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Kitaura, T. Kinoshita, M. Matsumoto, S. Chung, Y. Kawakami, M. Leitges, D. Wu, C. A. Lowell, and T. Kawakami IgE- and IgE+Ag-mediated mast cell migration in an autocrine/paracrine fashion Blood, April 15, 2005; 105(8): 3222 - 3229. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Yokoo, Y. Yatomi, T. Takafuta, M. Osada, Y. Okamoto, and Y. Ozaki Sphingosine 1-Phosphate Inhibits Migration of RBL-2H3 Cells via S1P2: Cross-Talk between Platelets and Mast Cells J. Biochem., June 1, 2004; 135(6): 673 - 681. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Oka, M. Hori, A. Tanaka, H. Matsuda, H. Karaki, and H. Ozaki IgE alone-induced actin assembly modifies calcium signaling and degranulation in RBL-2H3 mast cells Am J Physiol Cell Physiol, February 1, 2004; 286(2): C256 - C263. [Abstract] [Full Text]
|
|
|
|
 |
|
 D. Pradip, X. Peng, and D. L. Durden Rac2 Specificity in Macrophage Integrin Signaling: POTENTIAL ROLE FOR Syk KINASE J. Biol. Chem., October 24, 2003; 278(43): 41661 - 41669. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Kono, T. Suzuki, K. Yamamoto, M. Okada, T. Yamamoto, and Z.-i. Honda Spatial Raft Coalescence Represents an Initial Step in Fc{gamma}R Signaling J. Immunol., July 1, 2002; 169(1): 193 - 203. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Ueda, M. Mizuki, H. Ikeda, T. Tsujimura, I. Matsumura, K. Nakano, H. Daino, Z.-i. Honda, J. Sonoyama, H. Shibayama, et al. Critical roles of c-Kit tyrosine residues 567 and 719 in stem cell factor-induced chemotaxis: contribution of src family kinase and PI3-kinase on calcium mobilization and cell migration Blood, May 1, 2002; 99(9): 3342 - 3349. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Piccardoni, R. Sideri, S. Manarini, A. Piccoli, N. Martelli, G. de Gaetano, C. Cerletti, and V. Evangelista Platelet/polymorphonuclear leukocyte adhesion: a new role for SRC kinases in Mac-1 adhesive function triggered by P-selectin Blood, July 1, 2001; 98(1): 108 - 116. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Oda, H. D. Ochs, L. A. Lasky, S. Spencer, K. Ozaki, M. Fujihara, M. Handa, K. Ikebuchi, and H. Ikeda CrkL is an adapter for Wiskott-Aldrich syndrome protein and Syk Blood, May 1, 2001; 97(9): 2633 - 2639. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Yuasa, M. Ono, T. Watanabe, and T. Takai Lyn Is Essential for Fc{gamma} Receptor III-Mediated Systemic Anaphylaxis but Not for the Arthus Reaction J. Exp. Med., March 5, 2001; 193(5): 563 - 572. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Z. Korade-Mirnics and S. J. Corey Src kinase-mediated signaling in leukocytes J. Leukoc. Biol., November 1, 2000; 68(5): 603 - 613. [Abstract] [Full Text]
|
|
|
|
|
|
T. Suzuki, H. Kono, N. Hirose, M. Okada, T. Yamamoto, K. Yamamoto, and Z.-i. Honda Differential Involvement of Src Family Kinases in Fc{gamma} Receptor-Mediated Phagocytosis J. Immunol., July 1, 2000; 165(1): 473 - 482. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Z.-i. Honda, T. Suzuki, H. Kono, M. Okada, T. Yamamoto, C. Ra, Y. Morita, and K. Yamamoto Sequential Requirements of the N-Terminal Palmitoylation Site and SH2 Domain of Src Family Kinases in the Initiation and Progression of Fcvarepsilon RI Signaling Mol. Cell. Biol., March 1, 2000; 20(5): 1759 - 1771. [Abstract] [Full Text]
|
|
|
|
|
|
P. Lock, F. Casagranda, and A. R. Dunn Independent SH2-binding Sites Mediate Interaction of Dok-related Protein with RasGTPase-activating Protein and Nck J. Biol. Chem., August 6, 1999; 274(32): 22775 - 22784. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. V. Grishin, O. Azhipa, I. Semenov, and S. J. Corey Interaction between growth arrest-DNA damage protein 34 and Src kinase Lyn negatively regulates genotoxic apoptosis PNAS, August 28, 2001; 98(18): 10172 - 10177. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Geng, S. Pfister, S.-K. Kraeft, and C. E. Rudd Adaptor FYB (Fyn-binding protein) regulates integrin-mediated adhesion and mediator release: Differential involvement of the FYB SH3 domain PNAS, September 25, 2001; 98(20): 11527 - 11532. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
