| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
*
Division of Clinical Immunology, Mount Sinai Medical Center, New York, NY 10029; and
Department of Medicine, University of California at San Diego, Lo Jolla, CA 92093
Products of an activated immune system may affect cells within the
immune system as well as nonlymphoid cells in the local environment.
Given the immunologically activated state of the intestinal tract, it
is conceivable that locally produced cytokines could regulate
epithelial cell function. To assess whether epithelial cells are
targets for particular cytokines, we initiated studies on the binding
of a panel of proinflammatory cytokines in freshly isolated epithelial
cells from normal and inflammatory bowel disease (IBD) patients as well
as in cell lines. Isolated intestinal epithelial cells (IEC) were
stained with phycoerythrin-conjugated or biotinylated cytokines to
determine the expression and density of receptors for IL-1ß, IL-6,
granulocyte-macrophage CSF (GM-CSF), and TNF-
. Receptors for
IL-1ß, IL-6, and GM-CSF were readily detectable in all epithelial
cell preparations at levels equal to (GM-CSFR) or lower than those seen
on monocytes. However TNF-R were not detectable on freshly isolated
IECs. Receptor density was greater in surface vs crypt epithelial
cells, but no significant differences were seen between normal and IBD
epithelial cells. Expression of IL-1R and IL-6R was enhanced by LPS and
IFN-
. Functionally, IL-1ß enhanced proliferation of the IEC cell
line, DLD1, whereas GM-CSF treatment of de-differentiated crypt-like
DLD1 and HT29 cells resulted in enhanced expression of ICAM-1.
Furthermore, TNF- treatment enhanced the secretion of IL-8 and
GRO- in HT29 cells, but not in freshly isolated IEC cultures.
The differential binding and function of proinflammatory cytokines on
IEC support the hypothesis that these cytokines may be involved in
normal physiological processes as well as in regulating mucosal immune
responses.
This article has been cited by other articles:
|
|
 |
|
  R. Tammali, A. B. M. Reddy, K. V. Ramana, J. M. Petrash, and S. K. Srivastava Aldose reductase deficiency in mice prevents azoxymethane-induced colonic preneoplastic aberrant crypt foci formation Carcinogenesis, May 1, 2009; 30(5): 799 - 807. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Chen, J. N. Rao, T. Zou, L. Liu, B. S. Marasa, L. Xiao, X. Zeng, D. J. Turner, and J.-Y. Wang Polyamines are required for expression of Toll-like receptor 2 modulating intestinal epithelial barrier integrity Am J Physiol Gastrointest Liver Physiol, September 1, 2007; 293(3): G568 - G576. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. L. Theiss, T. S. Obertone, D. Merlin, and S. V. Sitaraman Interleukin-6 Transcriptionally Regulates Prohibitin Expression in Intestinal Epithelial Cells J. Biol. Chem., April 27, 2007; 282(17): 12804 - 12812. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Wang, S. Srinivasan, A. L. Theiss, D. Merlin, and S. V. Sitaraman Interleukin-6 Induces Keratin Expression in Intestinal Epithelial Cells: POTENTIAL ROLE OF KERATIN-8 IN INTERLEUKIN-6-INDUCED BARRIER FUNCTION ALTERATIONS J. Biol. Chem., March 16, 2007; 282(11): 8219 - 8227. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J R Korzenik Is Crohn's disease due to defective immunity? Gut, January 1, 2007; 56(1): 2 - 5. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Wen, S. F. Giardina, D. Hamming, J. Greenman, E. Zachariah, M. D. Bacolod, H. Liu, J. Shia, P. S. Amenta, F. Barany, et al. GRO{alpha} Is Highly Expressed in Adenocarcinoma of the Colon and Down-Regulates Fibulin-1. Clin. Cancer Res., October 15, 2006; 12(20): 5951 - 5959. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. M. Munoz, A. Y. Meliton, A. Lambertino, E. Boetticher, J. Learoyd, F. Sultan, X. Zhu, W. Cho, and A. R. Leff Transcellular Secretion of Group V Phospholipase A2 from Epithelium Induces beta2-Integrin-Mediated Adhesion and Synthesis of Leukotriene C4 in Eosinophils J. Immunol., July 1, 2006; 177(1): 574 - 582. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Chao, C. J. Connell, E. J. Jacobs, M. L. McCullough, A. V. Patel, E. E. Calle, V. E. Cokkinides, and M. J. Thun Amount, Type, and Timing of Recreational Physical Activity in Relation to Colon and Rectal Cancer in Older Adults: the Cancer Prevention Study II Nutrition Cohort Cancer Epidemiol. Biomarkers Prev., December 1, 2004; 13(12): 2187 - 2195. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Pie, J. P. Lalles, F. Blazy, J. Laffitte, B. Seve, and I. P. Oswald Weaning Is Associated with an Upregulation of Expression of Inflammatory Cytokines in the Intestine of Piglets J. Nutr., March 1, 2004; 134(3): 641 - 647. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Wang, B. Walia, J. Evans, A. T. Gewirtz, D. Merlin, and S. V. Sitaraman IL-6 Induces NF-{kappa}B Activation in the Intestinal Epithelia J. Immunol., September 15, 2003; 171(6): 3194 - 3201. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. T. Jubinsky, M. K. Short, G. Mutema, and D. P. Witte Developmental Expression of Magmas in Murine Tissues and Its Co-expression with the GM-CSF Receptor J. Histochem. Cytochem., May 1, 2003; 51(5): 585 - 596. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F M Ruemmele, J F Beaulieu, S Dionne, E Levy, E G Seidman, N Cerf-Bensussan, and M J Lentze Lipopolysaccharide modulation of normal enterocyte turnover by toll-like receptors is mediated by endogenously produced tumour necrosis factor {alpha} Gut, December 1, 2002; 51(6): 842 - 848. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. R. Companjen, L. I. v. d. Wel, L. Boon, E. P. Prens, and J. D. Laman CD40 ligation-induced cytokine production in human skin explants is partly mediated via IL-1 Int. Immunol., June 1, 2002; 14(6): 669 - 676. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Lacroix-Lamande, R. Mancassola, M. Naciri, and F. Laurent Role of Gamma Interferon in Chemokine Expression in the Ileum of Mice and in a Murine Intestinal Epithelial Cell Line after Cryptosporidium parvum Infection Infect. Immun., April 1, 2002; 70(4): 2090 - 2099. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. J. D. Mennechet, L. H. Kasper, N. Rachinel, W. Li, A. Vandewalle, and D. Buzoni-Gatel Lamina Propria CD4+ T Lymphocytes Synergize with Murine Intestinal Epithelial Cells to Enhance Proinflammatory Response Against an Intracellular Pathogen J. Immunol., March 15, 2002; 168(6): 2988 - 2996. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. P. Bradney, G. D. Sempowski, H.-X. Liao, B. F. Haynes, and H. F. Staats Cytokines as Adjuvants for the Induction of Anti-Human Immunodeficiency Virus Peptide Immunoglobulin G (IgG) and IgA Antibodies in Serum and Mucosal Secretions after Nasal Immunization J. Virol., January 15, 2002; 76(2): 517 - 524. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. A. Martin and A. Panja Cytokine regulation of human intestinal primary epithelial cell susceptibility to Fas-mediated apoptosis Am J Physiol Gastrointest Liver Physiol, January 1, 2002; 282(1): G92 - G104. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. S. Goncalves, M. Ghaem-Maghami, G. Monteleone, G. Frankel, G. Dougan, D. J. M. Lewis, C. P. Simmons, and T. T. MacDonald Critical Role for Tumor Necrosis Factor Alpha in Controlling the Number of Lumenal Pathogenic Bacteria and Immunopathology in Infectious Colitis Infect. Immun., November 1, 2001; 69(11): 6651 - 6659. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. F. Staats, C. P. Bradney, W. M. Gwinn, S. S. Jackson, G. D. Sempowski, H.-X. Liao, N. L. Letvin, and B. F. Haynes Cytokine Requirements for Induction of Systemic and Mucosal CTL After Nasal Immunization J. Immunol., November 1, 2001; 167(9): 5386 - 5394. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X Bo, U Broome, M Remberger, and S Sumitran-Holgersson Tumour necrosis factor {alpha} impairs function of liver derived T lymphocytes and natural killer cells in patients with primary sclerosing cholangitis Gut, July 1, 2001; 49(1): 131 - 141. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Wen and G. D. Wu Evidence for Epigenetic Mechanisms That Silence Both Basal and Immune-Stimulated Transcription of the IL-8 Gene J. Immunol., June 15, 2001; 166(12): 7290 - 7299. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S Shinozaki, T Nakamura, M Iimura, Y Kato, B Iizuka, M Kobayashi, and N Hayashi Upregulation of Reg 1{alpha} and GW112 in the epithelium of inflamed colonic mucosa Gut, May 1, 2001; 48(5): 623 - 629. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. G. Dickman, S. J. Hempson, J. Anderson, S. Lippe, L. Zhao, R. Burakoff, and R. D. Shaw Rotavirus alters paracellular permeability and energy metabolism in Caco-2 cells Am J Physiol Gastrointest Liver Physiol, October 1, 2000; 279(4): G757 - G766. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M Iimura, T Nakamura, S Shinozaki, B Iizuka, Y Inoue, S Suzuki, and N Hayashi Bax is downregulated in inflamed colonic mucosa of ulcerative colitis Gut, August 1, 2000; 47(2): 228 - 235. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D Haller, C Bode, W P Hammes, A M A Pfeifer, E J Schiffrin, and S Blum Non-pathogenic bacteria elicit a differential cytokine response by intestinal epithelial cell/leucocyte co-cultures Gut, July 1, 2000; 47(1): 79 - 87. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
U. Bocker, A. Schottelius, J. M. Watson, L. Holt, L. L. Licato, D. A. Brenner, R. B. Sartor, and C. Jobin Cellular Differentiation Causes a Selective Down-regulation of Interleukin (IL)-1beta -mediated NF-kappa B Activation and IL-8 Gene Expression in Intestinal Epithelial Cells J. Biol. Chem., April 14, 2000; 275(16): 12207 - 12213. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. E. Rollo, K. P. Kumar, N. C. Reich, J. Cohen, J. Angel, H. B. Greenberg, R. Sheth, J. Anderson, B. Oh, S. J. Hempson, et al. The Epithelial Cell Response to Rotavirus Infection J. Immunol., October 15, 1999; 163(8): 4442 - 4452. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Merendino, M. B. Dwinell, N. Varki, L. Eckmann, and M. F. Kagnoff Human intestinal epithelial cells express receptors for platelet-activating factor Am J Physiol Gastrointest Liver Physiol, October 1, 1999; 277(4): G810 - G818. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. Zhang, J. K. Kolls, P. Oliver, D. Good, P. O. Schwarzenberger, M. S. Joshi, J. L. Ponthier, and J. R. Lancaster Jr. Activation of Tumor Necrosis Factor-alpha -converting Enzyme-mediated Ectodomain Shedding by Nitric Oxide J. Biol. Chem., May 19, 2000; 275(21): 15839 - 15844. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
