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B Sites in Mouse CD95 Ligand (Fas Ligand) Promoter: Functional Analysis in T Cell Hybridoma1
Departments of
*
Pathology and Laboratory Medicine,
Medicine, and
Microbiology, Boston University School of Medicine, Boston, MA 02118
Fas ligand (FasL) gene expression is critically involved in
peripheral T cell tolerance and lymphocyte homeostasis. Previous
studies have suggested that nuclear translocation of NF-
B during T
cell activation is a critical event for FasL gene activation. In the
present study we have identified two NF-B sites (designated
FasL-B1 and FasL-B2) on the promoter (
700 bp) of FasL. The
NF-B sites were identified by electrophoretic mobility shift assay.
Transient transfection reporter analyses showed that the FasL promoter
activity was comparable between a construct that contains both sites
and a shorter construct (433 bp) that contains only the FasL-B1
site. Furthermore, elimination of FasL-B1 by site-directed
mutagenesis significantly inhibited FasL promoter activity. These
observations provide strong evidence that NF-B directly binds to the
FasL-B1 site and up-regulates FasL gene
expression.
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A. Qadri, C. G. Radu, J. Thatte, P. Cianga, B. T. Ober, R. J. Ober, and E. S. Ward A Role for the Region Encompassing the c'' Strand of a TCR V{alpha} Domain in T Cell Activation Events J. Immunol., July 15, 2000; 165(2): 820 - 829. [Abstract] [Full Text] [PDF]
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L. A. Norian, K. M. Latinis, S. L. Eliason, K. Lyson, C. Yang, T. Ratliff, and G. A. Koretzky The Regulation of CD95 (Fas) Ligand Expression in Primary T Cells: Induction of Promoter Activation in CD95LP-Luc Transgenic Mice J. Immunol., May 1, 2000; 164(9): 4471 - 4480. [Abstract] [Full Text] [PDF]
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K. Matsui, S. Xiao, A. Fine, and S.-T. Ju Role of Activator Protein-1 in TCR-Mediated Regulation of the Murine fasl Promoter J. Immunol., March 15, 2000; 164(6): 3002 - 3008. [Abstract] [Full Text] [PDF]
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F. Kuhnel, L. Zender, Y. Paul, M. K. Tietze, C. Trautwein, M. Manns, and S. Kubicka NFkappa B Mediates Apoptosis through Transcriptional Activation of Fas (CD95) in Adenoviral Hepatitis J. Biol. Chem., February 25, 2000; 275(9): 6421 - 6427. [Abstract] [Full Text] [PDF]
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J. Zhang, B. Ma, A. Marshak-Rothstein, and A. Fine Characterization of a Novel Cis-element That Regulates Fas Ligand Expression in Corneal Endothelial Cells J. Biol. Chem., September 10, 1999; 274(37): 26537 - 26542. [Abstract] [Full Text] [PDF]
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S.-C. Hsu, M. A. Gavrilin, M.-H. Tsai, J. Han, and M.-Z. Lai p38 Mitogen-activated Protein Kinase Is Involved in Fas Ligand Expression J. Biol. Chem., September 3, 1999; 274(36): 25769 - 25776. [Abstract] [Full Text] [PDF]
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J. C. Pratt, M. R. M. van den Brink, V. E. Igras, S. F. Walk, K. S. Ravichandran, and S. J. Burakoff Requirement for Shc in TCR-Mediated Activation of a T Cell Hybridoma J. Immunol., September 1, 1999; 163(5): 2586 - 2591. [Abstract] [Full Text] [PDF]
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I. Rivera-Walsh, M. E. Cvijic, G. Xiao, and S.-C. Sun The NF-kappa B Signaling Pathway Is Not Required for Fas Ligand Gene Induction but Mediates Protection from Activation-induced Cell Death J. Biol. Chem., August 11, 2000; 275(33): 25222 - 25230. [Abstract] [Full Text] [PDF]
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Y. Chen and M.-Z. Lai c-Jun NH2-terminal Kinase Activation Leads to a FADD-dependent but Fas Ligand-independent Cell Death in Jurkat T Cells J. Biol. Chem., March 9, 2001; 276(11): 8350 - 8357. [Abstract] [Full Text] [PDF]
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P. R. Mittelstadt and J. D. Ashwell Inhibition of AP-1 by the Glucocorticoid-inducible Protein GILZ J. Biol. Chem., July 27, 2001; 276(31): 29603 - 29610. [Abstract] [Full Text] [PDF]
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