| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
2,*
árek§
*
Institute of Molecular Genetics, Academy of Sciences of the Czech Republic,
Second Department of Ophthalmology, Charles University,
Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, and
§
Faculty of Nuclear Sciences and Physical Engineering, Prague, Czech Republic
The immunosuppressive effects of UV radiation have been well
documented. This suppression has been attributed to the action of the
cis form of urocanic acid (UCA), a photoproduct of
trans-UCA, a natural constituent of the skin. Here, we
show that mouse spleen cells preincubated with cis-UCA
have a diminished proliferative response to allogeneic cells in MLC and
to stimulation with anti-CD3 mAb. Cells preincubated with
cis-UCA also had a decreased ability to serve as APC and
to stimulate the proliferation of allogeneic lymphocytes in MLC.
Simultaneously, the production of IL-2 and IFN-
by cells
preincubated with cis-UCA was decreased. However, IL-10
gene expression and IL-10 protein secretion by spleen cells stimulated
in the presence of cis-UCA were significantly enhanced.
The principal cell population displaying the
cis-UCA-induced elevated production of IL-10 was
CD4+ T cells, which were shown to be a direct target of
cis-UCA action. This was also supported by the
observation that production of IL-10 by stimulated splenic non-T cells
or by macrophages was not altered by cis-UCA. The
enhanced production of IL-10 by activated CD4+ T cells may
represent a novel pathway of UVB radiation-induced,
cis-UCA-mediated immunosuppression. We suggest that the
elevated production of IL-10 by activated CD4+ T cells may
account for the suppressor T cell phenomena described in UV-irradiated
recipients.
This article has been cited by other articles:
|
|
 |
|
  M. Krulova, K. Pokorna, A. Lencova, J. Fric, A. Zajicova, M. Filipec, J. V. Forrester, and V. Holan A Rapid Separation of Two Distinct Populations of Mouse Corneal Epithelial Cells with Limbal Stem Cell Characteristics by Centrifugation on Percoll Gradient Invest. Ophthalmol. Vis. Sci., September 1, 2008; 49(9): 3903 - 3908. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Kaneko, U. Smetana-Just, M. Matsui, A. R. Young, S. John, M. Norval, and S. L. Walker cis-Urocanic Acid Initiates Gene Transcription in Primary Human Keratinocytes J. Immunol., July 1, 2008; 181(1): 217 - 224. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Rana, S. N. Byrne, L. J. MacDonald, C. Y.-Y. Chan, and G. M. Halliday Ultraviolet B Suppresses Immunity by Inhibiting Effector and Memory T Cells Am. J. Pathol., April 1, 2008; 172(4): 993 - 1004. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. P. Walterscheid, D. X. Nghiem, N. Kazimi, L. K. Nutt, D. J. McConkey, M. Norval, and S. E. Ullrich Cis-urocanic acid, a sunlight-induced immunosuppressive factor, activates immune suppression via the 5-HT2A receptor PNAS, November 14, 2006; 103(46): 17420 - 17425. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Rinaldi, P. Moroni, L. Leino, J. Laihia, M. J. Paape, and D. D. Bannerman Effect of cis-urocanic acid on bovine neutrophil generation of reactive oxygen species. J Dairy Sci, November 1, 2006; 89(11): 4188 - 4201. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
