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CUTTING EDGE |
Department of Pathology, University of Massachusetts Medical Center, Worcester, MA 01655
Lymphocytic choriomeningitis virus (LCMV), like many viruses,
induces a profound activation and expansion of CD8+ T
cells. In contrast, CD4+ T cells do not increase in total
number during the acute infection. We show here that mice infected with
LCMV have a low but detectable frequency (<1/300) of CD4+
T cells, as detected by IL-2 production in limiting dilution assays, to
each of two class II peptides during the peak of the acute LCMV
response and into long-term memory. However, during the peak of the
acute CD4+ T cell response, >20% of the CD4+
T cells secreted IFN-
after stimulation with PMA and ionomycin, and
>10% of the CD4+ T cells secreted IFN- after
stimulation with the LCMV peptides. Thus, these new sensitive assays
reveal a heretofore unappreciated, yet profound Ag-specific
CD4+ T cell response during viral
infections.
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