HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Bono, P. Articles by Jalkanen, S. Search for Related Content PubMed PubMed Citation Articles by Bono, P. Articles by Jalkanen, S.

Isolation, Structural Characterization, and Chromosomal Mapping of the Mouse Vascular Adhesion Protein-1 Gene and Promoter^{1 ,2}

Petri Bono^3,, Marko Salmi, David J. Smith, Ilona Leppänen, Nina Horelli-Kuitunen^§, Aarno Palotie^§ and Sirpa Jalkanen

^* MediCity Research Laboratories, University of Turku, National Public Health Institute, and BioTie Therapies, BioCity, Turku, Finland; and ^§ Laboratory Department of Helsinki University Hospital and Department of Clinical Chemistry, University of Helsinki, Helsinki, Finland

Vascular adhesion protein-1 (VAP-1) is an endothelial cell adhesion molecule which mediates lymphocyte binding to endothelial cells. The cloning of a mouse VAP-1 (mVAP-1) cDNA revealed that mVAP-1 is a novel 110/220 kDa transmembrane molecule with significant identity to copper-containing amine oxidases. In this work the nucleotide sequence and primary structure of the mVAP-1 gene was determined and the promoter region was structurally characterized. The isolated approximately 14.4-kb mVAP-1 gene consists of 4 exons and 3 introns. Primer extension analysis and 5' rapid amplification of cDNA ends revealed multiple transcription initiation sites in different tissues suggesting that the mVAP-1 transcription is differently regulated in different tissues. Analysis of the sequence immediately upstream of the detected transcription initiation sites showed no canonical TATA or CCAAT elements, but putative regulatory elements were found close to the detected transcription start sites. The cloning of the mVAP-1 gene reveals the first insight into the genomic organization of murine amine oxidases and will, by targeted disruption of the gene, allow us to understand better the importance of VAP-1 in leukocyte trafficking and monoamine oxidase activity for the function of the immune system.

This article has been cited by other articles:

				P. F. LALOR, C. TUNCER, C. WESTON, A. MARTIN-SANTOS, D. J. SMITH, and D. H. ADAMS Vascular Adhesion Protein-1 as a Potential Therapeutic Target in Liver Disease Ann. N.Y. Acad. Sci., September 1, 2007; 1110(1): 485 - 496. [Abstract] [Full Text] [PDF]

				A. Vega, P. Chacon, J. Monteseirin, R. El Bekay, M. Alvarez, G. Alba, J. Conde, J. Martin-Nieto, F. J. Bedoya, E. Pintado, et al. A new role for monoamine oxidases in the modulation of macrophage-inducible nitric oxide synthase gene expression J. Leukoc. Biol., June 1, 2004; 75(6): 1093 - 1101. [Abstract] [Full Text] [PDF]

				N. Mercier, M. Moldes, K. E. Hadri, and B. Feve Regulation of Semicarbazide-Sensitive Amine Oxidase Expression by Tumor Necrosis Factor-alpha in Adipocytes: Functional Consequences on Glucose Transport J. Pharmacol. Exp. Ther., March 1, 2003; 304(3): 1197 - 1208. [Abstract] [Full Text] [PDF]

				P. F. Lalor, S. Edwards, G. McNab, M. Salmi, S. Jalkanen, and D. H. Adams Vascular Adhesion Protein-1 Mediates Adhesion and Transmigration of Lymphocytes on Human Hepatic Endothelial Cells J. Immunol., July 15, 2002; 169(2): 983 - 992. [Abstract] [Full Text] [PDF]

				K. El Hadri, M. Moldes, N. Mercier, M. Andreani, J. Pairault, and B. Feve Semicarbazide-Sensitive Amine Oxidase in Vascular Smooth Muscle Cells: Differentiation-Dependent Expression and Role in Glucose Uptake Arterioscler. Thromb. Vasc. Biol., January 1, 2002; 22(1): 89 - 94. [Abstract] [Full Text] [PDF]

				J. S. Alexander and D. N. Granger Lymphocyte Trafficking Mediated by Vascular Adhesion Protein-1 : Implications for Immune Targeting and Cardiovascular Disease Circ. Res., June 23, 2000; 86(12): 1190 - 1192. [Full Text] [PDF]

				P. Bono, S. Jalkanen, and M. Salmi Mouse Vascular Adhesion Protein 1 Is a Sialoglycoprotein with Enzymatic Activity and Is Induced in Diabetic Insulitis Am. J. Pathol., November 1, 1999; 155(5): 1613 - 1624. [Abstract] [Full Text] [PDF]

				M. Moldes, B. Feve, and J. Pairault Molecular Cloning of a Major mRNA Species in Murine 3T3 Adipocyte Lineage. DIFFERENTIATION-DEPENDENT EXPRESSION, REGULATION, AND IDENTIFICATION AS SEMICARBAZIDE-SENSITIVE AMINE OXIDASE J. Biol. Chem., April 2, 1999; 274(14): 9515 - 9523. [Abstract] [Full Text] [PDF]