| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Department of Molecular Genetics and Microbiology, Graduate Program in Immunology and Virology, University of Massachusetts Medical School, Worcester, MA 01655
B cell-specific activator protein (BSAP)/Pax-5 is a paired domain
DNA-binding protein expressed in the developing nervous system, testis,
and in all B lineage cells, except terminally differentiated plasma
cells. BSAP regulates transcription of several genes expressed in B
cells and also the activity of the 3' IgH enhancer. As it has binding
sites within or 5' to the switch regions of nearly all Ig heavy chain C
region genes and also is known to increase transcription of the
germline 
RNA, BSAP has been hypothesized to be involved in
regulation of Ab class switch recombination. To directly examine the
effects of BSAP on isotype switching, we use a tetracycline-regulated
expression system to overexpress BSAP in the surface IgM+
I.29µ B cell line, a mouse cell line that can be induced to undergo
class switch recombination. We find that overexpression of BSAP
inhibits switching to IgA in I.29µ cells stimulated with LPS +
TGF-ß1 + nicotinamide, but enhances switching to IgE in
cells stimulated with LPS + IL-4 + nicotinamide. Parallel to its
effects on switching, overexpression of BSAP inhibits germline 
RNA
expression and the transcriptional activity of the germline
promoter, while enhancing activity of the germline promoter.
Proliferation of I.29µ cells is not affected in this system. The
possible mechanisms and significance of the effect of BSAP on isotype
switching are discussed.
This article has been cited by other articles:
|
|
 |
|
  G. A. Robichaud, J.-P. Perreault, and R. J. Ouellette Development of an isoform-specific gene suppression system: the study of the human Pax-5B transcriptional element Nucleic Acids Res., August 1, 2008; 36(14): 4609 - 4620. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Kishida, Y. Hiromura, M. Shin-Ya, H. Asada, H. Kuriyama, M. Sugai, A. Shimizu, Y. Yokota, T. Hama, J. Imanishi, et al. IL-21 Induces Inhibitor of Differentiation 2 and Leads to Complete Abrogation of Anaphylaxis in Mice J. Immunol., December 15, 2007; 179(12): 8554 - 8561. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. P. Bradley, D. A. Kaminski, A. H. F. M. Peters, T. Jenuwein, and J. Stavnezer The Histone Methyltransferase Suv39h1 Increases Class Switch Recombination Specifically to IgA J. Immunol., July 15, 2006; 177(2): 1179 - 1188. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Pawlitzky, C. V. Angeles, A. M. Siegel, M. L. Stanton, R. Riblet, and P. H. Brodeur Identification of a Candidate Regulatory Element within the 5' Flanking Region of the Mouse Igh Locus Defined by Pro-B Cell-Specific Hypersensitivity Associated with Binding of PU.1, Pax5, and E2A. J. Immunol., June 1, 2006; 176(11): 6839 - 6851. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Oppezzo, G. Dumas, A. I. Lalanne, B. Payelle-Brogard, C. Magnac, O. Pritsch, G. Dighiero, and F. Vuillier Different isoforms of BSAP regulate expression of AID in normal and chronic lymphocytic leukemia B cells Blood, March 15, 2005; 105(6): 2495 - 2503. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Gonda, M. Sugai, Y. Nambu, T. Katakai, Y. Agata, K. J. Mori, Y. Yokota, and A. Shimizu The Balance Between Pax5 and Id2 Activities Is the Key to AID Gene Expression J. Exp. Med., November 3, 2003; 198(9): 1427 - 1437. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Schaffer, E. C. Kim, X. Wu, H. Zan, L. Testoni, S. Salamon, A. Cerutti, and P. Casali Selective Inhibition of Class Switching to IgG and IgE by Recruitment of the HoxC4 and Oct-1 Homeodomain Proteins and Ku70/Ku86 to Newly Identified ATTT cis-Elements J. Biol. Chem., June 13, 2003; 278(25): 23141 - 23150. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Zhang Accessibility control and machinery of immunoglobulin class switch recombination J. Leukoc. Biol., March 1, 2003; 73(3): 323 - 332. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Suto, H. Nakajima, K. Hirose, K. Suzuki, S.-i. Kagami, Y. Seto, A. Hoshimoto, Y. Saito, D. C. Foster, and I. Iwamoto Interleukin 21 prevents antigen-induced IgE production by inhibiting germ line Cepsilon transcription of IL-4-stimulated B cells Blood, December 15, 2002; 100(13): 4565 - 4573. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K.-I Lin, C. Angelin-Duclos, T. C. Kuo, and K. Calame Blimp-1-Dependent Repression of Pax-5 Is Required for Differentiation of B Cells to Immunoglobulin M-Secreting Plasma Cells Mol. Cell. Biol., July 1, 2002; 22(13): 4771 - 4780. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. E. Drouin, C. E. Schrader, J. Stavnezer, and U. Hansen The Ubiquitously Expressed DNA-Binding Protein Late SV40 Factor Binds Ig Switch Regions and Represses Class Switching to IgA J. Immunol., March 15, 2002; 168(6): 2847 - 2856. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Laurencikiene, V. Deveikaite, and E. Severinson HS1,2 Enhancer Regulation of Germline {epsilon} and {gamma}2b Promoters in Murine B Lymphocytes: Evidence for Specific Promoter-Enhancer Interactions J. Immunol., September 15, 2001; 167(6): 3257 - 3265. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M.-J. Shi, S.-R. Park, P.-H. Kim, and J. Stavnezer Roles of Ets proteins, NF-{{kappa}}B and nocodazole in regulating induction of transcription of mouse germline Ig {{alpha}} RNA by transforming growth factor-{beta}1 Int. Immunol., June 1, 2001; 13(6): 733 - 746. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Tell, A. Zecca, L. Pellizzari, P. Spessotto, A. Colombatti, M. R. Kelley, G. Damante, and C. Pucillo An environment to nucleus' signaling system operates in B lymphocytes: redox status modulates BSAP/Pax-5 activation through Ref-1 nuclear translocation Nucleic Acids Res., March 1, 2000; 28(5): 1099 - 1105. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Stavnezer, S. P. Bradley, N. Rousseau, T. Pearson, A. Shanmugam, D. J. Waite, P. R. Rogers, and A. L. Kenter Switch Recombination in a Transfected Plasmid Occurs Preferentially in a B Cell Line That Undergoes Switch Recombination of Its Chromosomal Ig Heavy Chain Genes J. Immunol., August 15, 1999; 163(4): 2028 - 2040. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. G. Soro, P. Morales-A., J. A. Martinez-M., S. Morales-A., S. G. Copin, M. A. R. Marcos, and M.-L. Gaspar Differential Involvement of the Transcription Factor Blimp-1 in T Cell-Independent and -Dependent B Cell Differentiation to Plasma Cells J. Immunol., July 15, 1999; 163(2): 611 - 617. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M.-J. Shi and J. Stavnezer CBF{alpha}3 (AML2) Is Induced by TGF-{beta}1 to Bind and Activate the Mouse Germline Ig {alpha} Promoter J. Immunol., December 15, 1998; 161(12): 6751 - 6760. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
