| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
-Melanocyte-Stimulating Hormone Inhibits the Nuclear Transcription Factor NF-
B Activation Induced by Various Inflammatory Agents
Cytokine Research Laboratory, Department of Molecular Oncology, University of Texas M. D. Anderson Cancer Center, Houston, TX 77030
-Melanocyte-stimulating hormone (-MSH) is a
tridecapeptide found mainly in the brain, pituitary, and circulation.
It inhibits most forms of inflammation by a mechanism that is not
known. As most types of inflammation require activation of NF-
B, we
investigated the effect of -MSH on the activation of this
transcription factor by a wide variety of inflammatory stimuli.
Electrophoretic mobility shift assay showed that -MSH completely
abolished TNF-mediated NF-B activation in a dose- and time-dependent
manner. It also suppressed NF-B activation induced by LPS, okadaic
acid, and ceramide. The effect was specific, as the activation of the
transcription factor activating protein-1 by TNF was unaffected.
Western blot analysis revealed that TNF-dependent degradation of the
inhibitory subunit of NF-B, IB, and nuclear translocation of
the p65 subunit of NF-B were also inhibited. This correlated with
suppression of NF-B-dependent reporter gene expression induced by
TNF. The inhibitory effect of -MSH appeared to be mediated through
generation of cAMP, as inhibitors of adenylate cyclase and of protein
kinase A reversed its inhibitory effect. Similarly, addition of
membrane-permeable dibutyryl cAMP, like -MSH, suppressed TNF-induced
NF-B activation. Overall, our results suggest that -MSH
suppresses NF-B activated by various inflammatory agents and that
this mechanism probably contributes to its anti-inflammatory
effects.
This article has been cited by other articles:
|
|
 |
|
  G. Leoni, H. B. Patel, A. L. F. Sampaio, F. N. E. Gavins, J. F. Murray, P. Grieco, S. J. Getting, and M. Perretti Inflamed phenotype of the mesenteric microcirculation of melanocortin type 3 receptor-null mice after ischemia-reperfusion FASEB J, December 1, 2008; 22(12): 4228 - 4238. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Brzoska, T. A. Luger, C. Maaser, C. Abels, and M. Bohm {alpha}-Melanocyte-Stimulating Hormone and Related Tripeptides: Biochemistry, Antiinflammatory and Protective Effects in Vitro and in Vivo, and Future Perspectives for the Treatment of Immune-Mediated Inflammatory Diseases Endocr. Rev., August 1, 2008; 29(5): 581 - 602. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Li and A. W. Taylor Diminishment of {alpha}-MSH anti-inflammatory activity in MC1r siRNA-transfected RAW264.7 macrophages J. Leukoc. Biol., July 1, 2008; 84(1): 191 - 198. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. A Luger and T. Brzoska {alpha}-MSH related peptides: a new class of anti-inflammatory and immunomodulating drugs Ann Rheum Dis, November 1, 2007; 66(suppl_3): iii52 - iii55. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Catania The melanocortin system in leukocyte biology J. Leukoc. Biol., February 1, 2007; 81(2): 383 - 392. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. J. Getting, C. W. Lam, G. Leoni, F. N. E. Gavins, P. Grieco, and M. Perretti [D-Trp8]-{gamma}-Melanocyte-Stimulating Hormone Exhibits Anti-Inflammatory Efficacy in Mice Bearing a Nonfunctional MC1R (Recessive Yellow e/e Mouse) Mol. Pharmacol., December 1, 2006; 70(6): 1850 - 1855. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. J. Getting, C. W. Lam, A. S. Chen, P. Grieco, and M. Perretti Melanocortin 3 receptors control crystal-induced inflammation FASEB J, November 1, 2006; 20(13): 2234 - 2241. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Kang, K. W. McIntyre, K. M. Gillooly, Y. Yang, J. Haycock, S. Roberts, A. Khanna, T. F. Herpin, G. Yu, X. Wu, et al. A selective small molecule agonist of the melanocortin-1 receptor inhibits lipopolysaccharide-induced cytokine accumulation and leukocyte infiltration in mice J. Leukoc. Biol., October 1, 2006; 80(4): 897 - 904. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C Maaser, K Kannengiesser, C Specht, A Lugering, T Brzoska, T A Luger, W Domschke, and T Kucharzik Crucial role of the melanocortin receptor MC1R in experimental colitis Gut, October 1, 2006; 55(10): 1415 - 1422. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. R. Vulliemoz, E. Xiao, L. Xia-Zhang, M. Ferin, and S. L. Wardlaw Melanocortin Modulation of Inflammatory Cytokine and Neuroendocrine Responses to Endotoxin in the Monkey Endocrinology, April 1, 2006; 147(4): 1878 - 1883. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G.-S. Liu, L.-F. Liu, C.-J. Lin, J.-C. Tseng, M.-J. Chuang, H.-C. Lam, J.-K. Lee, L.-C. Yang, J. H. Y. Chan, S.-L. Howng, et al. Gene Transfer of Pro-opiomelanocortin Prohormone Suppressed the Growth and Metastasis of Melanoma: Involvement of {alpha}-Melanocyte-Stimulating Hormone-Mediated Inhibition of the Nuclear Factor {kappa}B/Cyclooxygenase-2 Pathway Mol. Pharmacol., February 1, 2006; 69(2): 440 - 451. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Deng, X. Hu, P. S. T. Yuen, and R. A. Star {alpha}-Melanocyte-stimulating Hormone Inhibits Lung Injury after Renal Ischemia/Reperfusion Am. J. Respir. Crit. Care Med., March 15, 2004; 169(6): 749 - 756. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Catania, S. Gatti, G. Colombo, and J. M. Lipton Targeting Melanocortin Receptors as a Novel Strategy to Control Inflammation Pharmacol. Rev., March 1, 2004; 56(1): 1 - 29. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Bohm, M. Raghunath, C. Sunderkotter, M. Schiller, S. Stander, T. Brzoska, T. Cauvet, H. B. Schioth, T. Schwarz, and T. A. Luger Collagen Metabolism Is a Novel Target of the Neuropeptide {alpha}-Melanocyte-stimulating Hormone J. Biol. Chem., February 20, 2004; 279(8): 6959 - 6966. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Catania, G. Colombo, A. Carlin, L. Garofalo, S. Gatti, R. Buffa, N. Carboni, L. Rosso, L. Santambrogio, L. Cantalamessa, et al. Autocrine inhibitory influences of {alpha}-melanocyte-stimulating hormone in malignant pleural mesothelioma J. Leukoc. Biol., February 1, 2004; 75(2): 253 - 259. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Shiratori, K. Ohgami, I. B. Ilieva, Y. Koyama, K. Yoshida, and S. Ohno Inhibition of Endotoxin-Induced Uveitis and Potentiation of Cyclooxygenase-2 Protein Expression by {alpha}-Melanocyte-Stimulating Hormone Invest. Ophthalmol. Vis. Sci., January 1, 2004; 45(1): 159 - 164. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Abeyama, K.-i. Kawahara, S. Iino, T. Hamada, S.-i. Arimura, K. Matsushita, T. Nakajima, and I. Maruyama Antibiotic cyclic AMP signaling by "primed" leukocytes confers anti-inflammatory cytoprotection J. Leukoc. Biol., November 1, 2003; 74(5): 908 - 915. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Xiao, L. Xia-Zhang, N. R. Vulliemoz, M. Ferin, and S. L. Wardlaw Leptin Modulates Inflammatory Cytokine and Neuroendocrine Responses to Endotoxin in the Primate Endocrinology, October 1, 2003; 144(10): 4350 - 4353. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S.-W. Yoon, S.-H. Goh, J.-S. Chun, E.-W. Cho, M.-K. Lee, K.-L. Kim, J.-J. Kim, C.-J. Kim, and H. Poo {alpha}-Melanocyte-stimulating Hormone Inhibits Lipopolysaccharide-induced Tumor Necrosis Factor-{alpha} Production in Leukocytes by Modulating Protein Kinase A, p38 Kinase, and Nuclear Factor {kappa}B Signaling Pathways J. Biol. Chem., August 29, 2003; 278(35): 32914 - 32920. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. J. Getting, H. B. Schioth, and M. Perretti Dissection of the Anti-Inflammatory Effect of the Core and C-Terminal (KPV) {alpha}-Melanocyte-Stimulating Hormone Peptides J. Pharmacol. Exp. Ther., August 1, 2003; 306(2): 631 - 637. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Xiao, L. Xia-Zhang, N. R. Vulliemoz, M. Ferin, and S. L. Wardlaw Agouti-Related Protein Stimulates the Hypothalamic-Pituitary-Adrenal (HPA) Axis and Enhances the HPA Response to Interleukin-1 in the Primate Endocrinology, May 1, 2003; 144(5): 1736 - 1741. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. J. Getting, H. C. Christian, C. W. Lam, F. N. E. Gavins, R. J. Flower, H. B. Schioth, and M. Perretti Redundancy of a Functional Melanocortin 1 Receptor in the Anti-inflammatory Actions of Melanocortin Peptides: Studies in the Recessive Yellow (e/e) Mouse Suggest an Important Role for Melanocortin 3 Receptor J. Immunol., March 15, 2003; 170(6): 3323 - 3330. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. E. Scholzen, C. Sunderkotter, D.-H. Kalden, T. Brzoska, M. Fastrich, T. Fisbeck, C. A. Armstrong, J. C. Ansel, and T. A. Luger {alpha}-Melanocyte Stimulating Hormone Prevents Lipopolysaccharide-Induced Vasculitis by Down-Regulating Endothelial Cell Adhesion Molecule Expression Endocrinology, January 1, 2003; 144(1): 360 - 370. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Gavrilyuk, C. Dello Russo, M. T. Heneka, D. Pelligrino, G. Weinberg, and D. L. Feinstein Norepinephrine Increases Ikappa Balpha Expression in Astrocytes J. Biol. Chem., August 9, 2002; 277(33): 29662 - 29668. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. R. Bernik, S. G. Friedman, M. Ochani, R. DiRaimo, L. Ulloa, H. Yang, S. Sudan, C. J. Czura, S. M. Ivanova, and K. J. Tracey Pharmacological Stimulation of the Cholinergic Antiinflammatory Pathway J. Exp. Med., March 18, 2002; 195(6): 781 - 788. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Ichiyama, M. Nishikawa, T. Yoshitomi, S. Hasegawa, T. Matsubara, T. Hayashi, and S. Furukawa Clarithromycin Inhibits NF-{kappa}B Activation in Human Peripheral Blood Mononuclear Cells and Pulmonary Epithelial Cells Antimicrob. Agents Chemother., January 1, 2001; 45(1): 44 - 47. [Abstract] [Full Text]
|
|
|
|
|
|
S. J. Getting, G. H. Allcock, R. Flower, and M. Perretti Natural and synthetic agonists of the melanocortin receptor type 3 possess anti-inflammatory properties J. Leukoc. Biol., January 1, 2001; 69(1): 98 - 104. [Abstract] [Full Text]
|
|
|
|
|
|
W. Barcellini, G. Colombo, L. La Maestra, G. Clerici, L. Garofalo, A. T. Brini, J. M. Lipton, and A. Catania {alpha}-Melanocyte-stimulating hormone peptides inhibit HIV-1 expression in chronically infected promonocytic U1 cells and in acutely infected monocytes J. Leukoc. Biol., November 1, 2000; 68(5): 693 - 699. [Abstract] [Full Text]
|
|
|
|
 |
|
 N. Ouchi, S. Kihara, Y. Arita, Y. Okamoto, K. Maeda, H. Kuriyama, K. Hotta, M. Nishida, M. Takahashi, M. Muraguchi, et al. Adiponectin, an Adipocyte-Derived Plasma Protein, Inhibits Endothelial NF-{kappa}B Signaling Through a cAMP-Dependent Pathway Circulation, September 12, 2000; 102(11): 1296 - 1301. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Airaghi, L. Garofalo, M. G. Cutuli, R. Delgado, A. Carlin, M. T. Demitri, S. Badalamenti, G. Graziani, J. M. Lipton, and A. Catania Plasma concentrations of {alpha}-melanocyte-stimulating hormone are elevated in patients on chronic haemodialysis Nephrol. Dial. Transplant., August 1, 2000; 15(8): 1212 - 1216. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Slominski, J. Wortsman, T. Luger, R. Paus, and S. Solomon Corticotropin Releasing Hormone and Proopiomelanocortin Involvement in the Cutaneous Response to Stress Physiol Rev, July 1, 2000; 80(3): 979 - 1020. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. K. Manna, N. K. Sah, R. A. Newman, A. Cisneros, and B. B. Aggarwal Oleandrin Suppresses Activation of Nuclear Transcription Factor-{{kappa}}B, Activator Protein-1, and c-Jun NH2-Terminal Kinase Cancer Res., July 1, 2000; 60(14): 3838 - 3847. [Abstract] [Full Text]
|
|
|
|
|
|
S. K. Manna, A. Mukhopadhyay, and B. B. Aggarwal Human Chorionic Gonadotropin Suppresses Activation of Nuclear Transcription Factor-kappa B and Activator Protein-1 Induced by Tumor Necrosis Factor J. Biol. Chem., April 28, 2000; 275(18): 13307 - 13314. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Delgado and D. Ganea Vasoactive Intestinal Peptide and Pituitary Adenylate Cyclase-activating Polypeptide Inhibit Interleukin-12 Transcription by Regulating Nuclear Factor kappa B and Ets Activation J. Biol. Chem., November 5, 1999; 274(45): 31930 - 31940. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Adachi, T. Nakano, H. Vliagoftis, and D. D. Metcalfe Receptor-Mediated Modulation of Murine Mast Cell Function by {alpha}-Melanocyte Stimulating Hormone J. Immunol., September 15, 1999; 163(6): 3363 - 3368. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
