| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Versus IL-4 and IL-10: Selective Induction of IL-10 by Sequential Stimulation of Naive Th Cells with IL-12 and IL-41
*
Institute for Genetics, University of Cologne, Zentrum für Molekularbiologische Medizin, Cologne, Germany, and Deutsches Rheuma-Forschungszentrum, Berlin, Germany; and
Miltenyi Biotec GmbH, Bergisch Gladbach, Germany
Commitment of Th lymphocytes to the Th1 phenotype, as characterized
by the expression of the major proinflammatory cytokine IFN-
, may be
critically involved in the establishment of chronic inflammation and
inflammatory autoimmune disease. To date, it has been shown that in
IL-12-stimulated murine Th cell lines containing a major fraction of
Th1 cells, Th2 cells can be induced by IL-4 until about 2 wk after
initial activation, but not later. Here we analyze, based on the
magnetic isolation of viable Th1 cells according to their specific
expression of IFN-, the cytokine commitment of individual Th1 cells.
After activation of naive Th cells with Ag and IL-12 for up to 5 wk,
isolated IFN--producing cells were restimulated with Ag and IL-4.
Within the first 3 to 4 wk of IL-12 stimulation, some
IFN-+ cells stopped expression of IFN- when
restimulated with IL-4. However, within only 1 to 2 wk of IL-12
stimulation, few IFN-+ cells could be converted to
produce IL-4. Others continued to express IFN- and thus were already
committed to a proinflammatory, Th1-like phenotype. Surprisingly,
within 3 wk of IL-12 stimulation, many of the IFN--producing cells
responded to IL-4 restimulation by expression of IL-10, but neither
IFN- nor IL-4, i.e., by conversion to a suppressive,
anti-inflammatory phenotype.
This article has been cited by other articles:
|
|
 |
|
  F. Blumenthal-Barby, K. Eulenburg, A. Schrage, M. Zeitz, A. Hamann, and K. Klugewitz In vivo modulation of antigen-experienced cells in response to high-dose oral antigen: deletion but no evidence for alterations in the cytokine phenotype Int. Immunol., July 1, 2008; 20(7): 893 - 900. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Langer, D. Langnickel, P. Enghard, R. Undeutsch, G. R. Burmester, F. Hiepe, A. Radbruch, and G. Riemekasten The systemic and SmD183-119-autoantigen-specific cytokine memory of Th cells in SLE patients Rheumatology, February 1, 2007; 46(2): 238 - 245. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Negishi, Y. Kato, O. Ooneda, J. Mimura, T. Takada, H. Mochizuki, M. Yamamoto, Y. Fujii-Kuriyama, and S. Furusako Effects of Aryl Hydrocarbon Receptor Signaling on the Modulation of Th1/Th2 Balance J. Immunol., December 1, 2005; 175(11): 7348 - 7356. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L.-O. Tykocinski, P. Hajkova, H.-D. Chang, T. Stamm, O. SOzeri, M. LOhning, J. Hu-Li, U. Niesner, S. Kreher, B. Friedrich, et al. A Critical Control Element for Interleukin-4 Memory Expression in T Helper Lymphocytes J. Biol. Chem., August 5, 2005; 280(31): 28177 - 28185. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Lohning, A. Richter, T. Stamm, J. Hu-Li, M. Assenmacher, W. E. Paul, and A. Radbruch Establishment of memory for IL-10 expression in developing T helper 2 cells requires repetitive IL-4 costimulation and does not impair proliferation PNAS, October 14, 2003; 100(21): 12307 - 12312. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. J. Loza and B. Perussia Peripheral Immature CD2-/low T Cell Development from Type 2 to Type 1 Cytokine Production J. Immunol., September 15, 2002; 169(6): 3061 - 3068. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. E. Wigginton and D. Kirschner A Model to Predict Cell-Mediated Immune Regulatory Mechanisms During Human Infection with Mycobacterium tuberculosis J. Immunol., February 1, 2001; 166(3): 1951 - 1967. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Jonuleit, E. Schmitt, G. Schuler, J. Knop, and A. H. Enk Induction of Interleukin 10-producing, Nonproliferating CD4+ T Cells with Regulatory Properties by Repetitive Stimulation with Allogeneic Immature Human Dendritic Cells J. Exp. Med., October 30, 2000; 192(9): 1213 - 1222. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. Yin, D.-H. Zhang, T. Welte, G. Bahtiyar, S. Jung, L. Liu, X.-Y. Fu, A. Ray, and J. Craft Dominance of IL-12 Over IL-4 in {gamma}{delta} T Cell Differentiation Leads to Default Production of IFN-{gamma}: Failure to Down-Regulate IL-12 Receptor {beta}2-Chain Expression J. Immunol., March 15, 2000; 164(6): 3056 - 3064. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Richter, M. Lohning, and A. Radbruch Instruction for Cytokine Expression in T Helper Lymphocytes in Relation to Proliferation and Cell Cycle Progression J. Exp. Med., November 15, 1999; 190(10): 1439 - 1450. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. G. Doyle, K. Buttigieg, P. Groves, B. J. Johnson, and A. Kelso The Activated Type 1-polarized CD8+ T Cell Population Isolated from an Effector Site Contains Cells with Flexible Cytokine Profiles J. Exp. Med., October 18, 1999; 190(8): 1081 - 1092. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Lohning, J. L. Grogan, A. J. Coyle, M. Yazdanbakhsh, C. Meisel, J.-C. Gutierrez-Ramos, A. Radbruch, and T. Kamradt T1/ST2 Expression Is Enhanced on CD4+ T Cells from Schistosome Egg-Induced Granulomas: Analysis of Th Cell Cytokine Coexpression Ex Vivo J. Immunol., April 1, 1999; 162(7): 3882 - 3889. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
