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*
Intestinal Disease Research Program, McMaster University, Hamilton, Canada;
Institute of Neurobiology, University of Amsterdam, Amsterdam, The Netherlands; and
Department of Pathology, Osaka University Medical School, Japan
Luminal Ag challenge of intestinal segments from sensitized rats
results in a rapid (
3 min) secretory response. We previously showed
in horseradish peroxidase (HRP)-sensitized rats that the initial phase
of transepithelial Ag transport occurred via a transcellular route and
was enhanced by sensitization. However, following the hypersensitivity
reaction, Ag also crossed between epithelial cells. The aim of this
study was to determine the role of mast cells in the altered
transepithelial Ag transport. White spotting mast cell-deficient
rats and +/+ littermate controls were sensitized to HRP. After 10 to 14
days, jejunal segments were resected, mounted in Ussing chambers, and
challenged with HRP on the luminal side. Electron microscopy of jejunum
fixed at 2 min showed a similarly enhanced endocytic transport of HRP
in sensitized +/+ and Ws/Ws rats compared with naive controls. In
sensitized +/+ rats, a secretory response occurred 3 min after
challenge, and tissue conductance increased thereafter. Naive +/+ and
sensitized Ws/Ws rats did not demonstrate a secretory response to HRP
challenge, and conductance remained at baseline levels. The flux of HRP
was elevated across tissue from sensitized +/+ rats but not across
tissue from naive controls or sensitized Ws/Ws rats. The results
indicate that sensitization enhances the initial phase of
transepithelial uptake of Ag by transcytosis in a mast cell-independent
manner. However, subsequent recruitment of the paracellular pathway for
Ag transport in sensitized rats is dependent upon the presence of mast
cells and occurs after the activation of such
cells.
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