| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
* Division of Respiratory Medicine, City Hospital, University of Nottingham, Nottingham, United Kingdom
IL-8 is an important neutrophil and eosinophil chemoattractant in asthma. A recent report has suggested that bradykinin (BK), an asthmatic mediator, induces the release of IL-8 in nonairway cells. We have recently reported that BK causes cyclooxygenase (COX)-2 induction and PGE2 release in human airway smooth muscle (ASM) cells. In this study, we tested the ability of BK to induce IL-8 from these cells and explored the role of COX products and COX-2 induction in this process. Confluent serum-deprived human ASM cells were studied. IL-8 was assayed by specific ELISA. Unstimulated cells released low levels of IL-8. BK enhanced IL-8 release in a concentration- and time-dependent fashion (maximum 50-fold increase over basal). The nonselective COX inhibitor indomethacin and the selective COX-2 inhibitor NS-398 strongly inhibited BK-stimulated PGE2 and IL-8 production. The COX substrate arachidonic acid also caused PGE2 and IL-8 production, and its effect was inhibited by nonselective COX inhibitors but unaffected by NS-398. Both the BK- and arachidonic acid-induced IL-8 production was inhibited by the protein synthesis inhibitors cycloheximide and actinomycin D and by the steroid dexamethasone. Furthermore, exogenous PGE2 and calcium ionophore A23187 also stimulated IL-8 release. BK-induced IL-8 release was mimicked by the BK B2 receptor agonist (Tyr(Me)8)-BK and was potently inhibited by the selective B2 receptor antagonist HOE-140. These results suggest that human ASM can be a source of IL-8 and also that endogenous prostanoids, involving both COX-1 and COX-2, have a novel role in mediating BK-induced IL-8 production.
This article has been cited by other articles:
|
|
 |
|
  S.-C. Ho, K.-Y. Lee, Y.-F. Chan, L.-W. Kuo, K. Ito, I. M. Adcock, B.-C. Chen, J.-R. Sheu, C.-H. Lin, and H.-P. Kuo Neutrophil Elastase Represses IL-8/CXCL8 Synthesis in Human Airway Smooth Muscle Cells through Induction of NF-{kappa}B Repressing Factor J. Immunol., July 1, 2009; 183(1): 411 - 420. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Kaur, N. S. Holden, S. M. Wilson, M. B. Sukkar, K. F. Chung, P. J. Barnes, R. Newton, and M. A. Giembycz Effect of {beta}2-adrenoceptor agonists and other cAMP-elevating agents on inflammatory gene expression in human ASM cells: a role for protein kinase A Am J Physiol Lung Cell Mol Physiol, September 1, 2008; 295(3): L505 - L514. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. F. Chung and I. M. Adcock Multifaceted mechanisms in COPD: inflammation, immunity, and tissue repair and destruction Eur. Respir. J., June 1, 2008; 31(6): 1334 - 1356. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. S. Mullan, M. Riley, D. Clarke, A. Tatler, A. Sutcliffe, A. J. Knox, and L. Pang {beta}-Tryptase Regulates IL-8 Expression in Airway Smooth Muscle Cells by a PAR-2-Independent Mechanism Am. J. Respir. Cell Mol. Biol., May 1, 2008; 38(5): 600 - 608. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. B. Penn and J. L. Benovic Regulation of Heterotrimeric G Protein Signaling in Airway Smooth Muscle Proceedings of the ATS, January 1, 2008; 5(1): 47 - 57. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Berndt, F. J. Derksen, P. J. Venta, S. Ewart, V. Yuzbasiyan-Gurkan, and N. E. Robinson Elevated amount of Toll-like receptor 4 mRNA in bronchial epithelial cells is associated with airway inflammation in horses with recurrent airway obstruction Am J Physiol Lung Cell Mol Physiol, April 1, 2007; 292(4): L936 - L943. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. G. Arndt, S. K. Young, K. R. Poch, J. A. Nick, S. Falk, R. W. Schrier, and G. S. Worthen Systemic Inhibition of the Angiotensin-Converting Enzyme Limits Lipopolysaccharide-Induced Lung Neutrophil Recruitment through Both Bradykinin and Angiotensin II-Regulated Pathways J. Immunol., November 15, 2006; 177(10): 7233 - 7241. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. Govindaraju, M.-C. Michoud, M. Al-Chalabi, P. Ferraro, W. S. Powell, and J. G. Martin Interleukin-8: novel roles in human airway smooth muscle cell contraction and migration Am J Physiol Cell Physiol, November 1, 2006; 291(5): C957 - C965. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Pang, M. Nie, L. Corbett, A. Sutcliffe, and A. J. Knox Mast Cell beta-Tryptase Selectively Cleaves Eotaxin and RANTES and Abrogates Their Eosinophil Chemotactic Activities J. Immunol., March 15, 2006; 176(6): 3788 - 3795. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. F. Chung The Role of Airway Smooth Muscle in the Pathogenesis of Airway Wall Remodeling in Chronic Obstructive Pulmonary Disease Proceedings of the ATS, November 1, 2005; 2(4): 347 - 354. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Bronner Is the upregulation of bradykinin B2 receptors by TGF-{beta}1 one of the missing pieces in the "airway hyperresponsiveness" puzzle? Am J Physiol Lung Cell Mol Physiol, October 1, 2005; 289(4): L509 - L510. [Full Text] [PDF]
|
|
|
|
 |
|
 M. Nie, L. Corbett, A. J. Knox, and L. Pang Differential Regulation of Chemokine Expression by Peroxisome Proliferator-activated Receptor {gamma} Agonists: INTERACTIONS WITH GLUCOCORTICOIDS AND {beta}2-AGONISTS J. Biol. Chem., January 28, 2005; 280(4): 2550 - 2561. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. A. Panettieri Jr. Effects of Corticosteroids on Structural Cells in Asthma and Chronic Obstructive Pulmonary Disease Proceedings of the ATS, November 1, 2004; 1(3): 231 - 234. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B.-C. Chen, C.-C. Yu, H.-C. Lei, M.-S. Chang, M.-J. Hsu, C.-L. Huang, M.-C. Chen, J.-R. Sheu, T.-F. Chen, T.-L. Chen, et al. Bradykinin B2 Receptor Mediates NF-{kappa}B Activation and Cyclooxygenase-2 Expression via the Ras/Raf-1/ERK Pathway in Human Airway Epithelial Cells J. Immunol., October 15, 2004; 173(8): 5219 - 5228. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. El-Haroun, D. Bradbury, A. Clayton, and A. J. Knox Interleukin-1{beta}, Transforming Growth Factor-{beta}1, and Bradykinin Attenuate Cyclic AMP Production by Human Pulmonary Artery Smooth Muscle Cells in Response to Prostacyclin Analogues and Prostaglandin E2 by Cyclooxygenase-2 Induction and Downregulation of Adenylyl Cyclase Isoforms 1, 2, and 4 Circ. Res., February 20, 2004; 94(3): 353 - 361. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Nie, L. Pang, H. Inoue, and A. J Knox Transcriptional Regulation of Cyclooxygenase 2 by Bradykinin and Interleukin-1{beta} in Human Airway Smooth Muscle Cells: Involvement of Different Promoter Elements, Transcription Factors, and Histone H4 Acetylation Mol. Cell. Biol., December 15, 2003; 23(24): 9233 - 9244. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. M. Zhu, D. A. Bradbury, L. Pang, and A. J. Knox Transcriptional Regulation of Interleukin (IL)-8 by Bradykinin in Human Airway Smooth Muscle Cells Involves Prostanoid-dependent Activation of AP-1 and Nuclear Factor (NF)-IL-6 and Prostanoid-independent Activation of NF-{kappa}B J. Biol. Chem., August 1, 2003; 278(31): 29366 - 29375. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Baraldo, D. S. Faffe, P. E. Moore, T. Whitehead, M. McKenna, E. S. Silverman, R. A. Panettieri Jr., and S. A. Shore Interleukin-9 influences chemokine release in airway smooth muscle: role of ERK Am J Physiol Lung Cell Mol Physiol, June 1, 2003; 284(6): L1093 - L1102. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Kumar, A. J. Knox, and A. M. Boriek CCAAT/Enhancer-binding Protein and Activator Protein-1 Transcription Factors Regulate the Expression of Interleukin-8 through the Mitogen-activated Protein Kinase Pathways in Response to Mechanical Stretch of Human Airway Smooth Muscle Cells J. Biol. Chem., May 23, 2003; 278(21): 18868 - 18876. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C.-D. Huang, O. Tliba, R. A. Panettieri Jr., and Y. Amrani Bradykinin Induces Interleukin-6 Production in Human Airway Smooth Muscle Cells: Modulation by Th2 Cytokines and Dexamethasone Am. J. Respir. Cell Mol. Biol., March 1, 2003; 28(3): 330 - 338. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Pang, M. Nie, L. Corbett, and A. J. Knox Cyclooxygenase-2 Expression by Nonsteroidal Anti-inflammatory Drugs in Human Airway Smooth Muscle Cells: Role of Peroxisome Proliferator-Activated Receptors J. Immunol., January 15, 2003; 170(2): 1043 - 1051. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. T. Gerthoffer and C. A. Singer Secretory Functions of Smooth Muscle: Cytokines and Growth Factors Mol. Interv., November 1, 2002; 2(7): 447 - 456. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. C. Rodgers, L. Pang, E. Holland, L. Corbett, S. Range, and A. J. Knox Bradykinin increases IL-8 generation in airway epithelial cells via COX-2-derived prostanoids Am J Physiol Lung Cell Mol Physiol, September 1, 2002; 283(3): L612 - L618. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Page, A. J. Ammit, J. L. Black, and C. L. Armour Human mast cell and airway smooth muscle cell interactions: implications for asthma Am J Physiol Lung Cell Mol Physiol, December 1, 2001; 281(6): L1313 - L1323. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. J. KNOX, L. CORBETT, J. STOCKS, E. HOLLAND, Y. M. ZHU, and L. PANG Human airway smooth muscle cells secrete vascular endothelial growth factor: up-regulation by bradykinin via a protein kinase C and prostanoid-dependent mechanism FASEB J, November 1, 2001; 15(13): 2480 - 2488. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L PANG COX-2 expression in asthmatic airways: the story so far Thorax, May 1, 2001; 56(5): 335 - 336. [Full Text]
|
|
|
|
|
|
L. PANG and A. J. KNOX Regulation of TNF-{alpha}-induced eotaxin release from cultured human airway smooth muscle cells by {beta}2-agonists and corticosteroids FASEB J, January 1, 2001; 15(1): 261 - 269. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C.-G. Yoo, S. Lee, C.-T. Lee, Y. W. Kim, S. K. Han, and Y.-S. Shim Effect of acetylsalicylic acid on endogenous I{kappa}B kinase activity in lung epithelial cells Am J Physiol Lung Cell Mol Physiol, January 1, 2001; 280(1): L3 - L9. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Pang and A. J. Knox Synergistic Inhibition by beta 2-Agonists and Corticosteroids on Tumor Necrosis Factor-alpha -Induced Interleukin-8 Release from Cultured Human Airway Smooth-Muscle Cells Am. J. Respir. Cell Mol. Biol., July 1, 2000; 23(1): 79 - 85. [Abstract] [Full Text]
|
|
|
|
|
|
C. Y. Fong, L. Pang, E. Holland, and A. J. Knox TGF-beta 1 stimulates IL-8 release, COX-2 expression, and PGE2 release in human airway smooth muscle cells Am J Physiol Lung Cell Mol Physiol, July 1, 2000; 279(1): L201 - L207. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Johnson and A. Knox Autocrine production of matrix metalloproteinase-2 is required for human airway smooth muscle proliferation Am J Physiol Lung Cell Mol Physiol, December 1, 1999; 277(6): L1109 - L1117. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. M. Hamad, S. R. Johnson, and A. J. Knox Antiproliferative effects of NO and ANP in cultured human airway smooth muscle Am J Physiol Lung Cell Mol Physiol, November 1, 1999; 277(5): L910 - L918. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
