| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
-Activated Endothelium Under Flow In Vitro1
*
Vascular Research Division, Departments of Pathology, Brigham and Women’s Hospital and Harvard Medical School, Boston, MA 02115;
Department of Immunology and Microbiology, Northwestern University School of Medicine, Chicago, IL 60611; and
Drug Discovery Group, Genetics Institute, Cambridge, MA 02140
In this study, an in vitro flow model and a blocking mAb to
P-selectin glycoprotein ligand-1 (PSGL-1) were used to define the role
of PSGL-1 in monocyte attachment and rolling on E- and P-selectin and
in attachment and accumulation on 6-h TNF-
-activated HUVEC. KPL1, an
adhesion-blocking mAb directed against the tyrosine sulfate motif of
PSGL-1, abolished monocyte-adhesive interactions with P-selectin, but
only partially blocked monocyte interaction with E-selectin. Further
analysis showed that on E-selectin, KPL1 blocked only secondary (i.e.,
monocyte/monocyte) interactions, but did not block primary (i.e.,
monocyte/E-selectin) interactions, with secondary adhesion accounting
for 90% of the total adhesive interactions on either E- or P-selectin.
On cytokine-activated HUVEC, monocytes initially attached and formed
linear strings of adherent cells, which involved both primary and
secondary adhesion. PSGL-1 or L-selectin mAb reduced string formation,
and the combination of PSGL-1 and L-selectin mAb prevented monocyte
strings and inhibited 86% of accumulation. Monocyte attachment and
rolling on purified adherent monocytes were also critically dependent
on PSGL-1 on the adherent monocytes. These studies document that
secondary interactions between monocytes, mediated by PSGL-1, are
crucial for monocyte initial attachment, rolling, and accumulation on
activated endothelium under laminar shear flow.
This article has been cited by other articles:
|
|
 |
|
  M. Stefanidakis, G. Newton, W. Y. Lee, C. A. Parkos, and F. W. Luscinskas Endothelial CD47 interaction with SIRP{gamma} is required for human T-cell transendothelial migration under shear flow conditions in vitro Blood, August 15, 2008; 112(4): 1280 - 1289. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. C. Kerr, C. B. Fieger, K. R. Snapp, and S. D. Rosen Endoglycan, a Member of the CD34 Family of Sialomucins, Is a Ligand for the Vascular Selectins J. Immunol., July 15, 2008; 181(2): 1480 - 1490. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. An, H. Wang, R. Tang, T. Yago, J. M. McDaniel, S. McGee, Y. Huo, and L. Xia P-Selectin Glycoprotein Ligand-1 Is Highly Expressed on Ly-6Chi Monocytes and a Major Determinant for Ly-6Chi Monocyte Recruitment to Sites of Atherosclerosis in Mice Circulation, June 24, 2008; 117(25): 3227 - 3237. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Xu, C. L. Cheng, M. Chen, A. Manivannan, L. Cabay, R. G. Pertwee, A. Coutts, and J. V. Forrester Anti-inflammatory property of the cannabinoid receptor-2-selective agonist JWH-133 in a rodent model of autoimmune uveoretinitis J. Leukoc. Biol., September 1, 2007; 82(3): 532 - 541. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Sircar, P. F. Bradfield, M. Aurrand-Lions, R. J. Fish, P. Alcaide, L. Yang, G. Newton, D. Lamont, S. Sehrawat, T. Mayadas, et al. Neutrophil Transmigration under Shear Flow Conditions In Vitro Is Junctional Adhesion Molecule-C Independent J. Immunol., May 1, 2007; 178(9): 5879 - 5887. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Kipar, H. May, S. Menger, M. Weber, W. Leukert, and M. Reinacher Morphologic Features and Development of Granulomatous Vasculitis in Feline Infectious Peritonitis Vet. Pathol., May 1, 2005; 42(3): 321 - 330. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. P. Burns and N. DePaola Flow-conditioned HUVECs support clustered leukocyte adhesion by coexpressing ICAM-1 and E-selectin Am J Physiol Heart Circ Physiol, January 1, 2005; 288(1): H194 - H204. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. K. Shaw, S. Ma, M. B. Kim, R. M. Rao, C. U. Hartman, R. M. Froio, L. Yang, T. Jones, Y. Liu, A. Nusrat, et al. Coordinated Redistribution of Leukocyte LFA-1 and Endothelial Cell ICAM-1 Accompany Neutrophil Transmigration J. Exp. Med., December 20, 2004; 200(12): 1571 - 1580. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. W. Homeister, A. Daugherty, and J. B. Lowe {alpha}(1,3)Fucosyltransferases FucT-IV and FucT-VII Control Susceptibility to Atherosclerosis in Apolipoprotein E-/- Mice Arterioscler Thromb Vasc Biol, October 1, 2004; 24(10): 1897 - 1903. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. M. Rao, T. V. Betz, D. J. Lamont, M. B. Kim, S. K. Shaw, R. M. Froio, F. Baleux, F. Arenzana-Seisdedos, R. Alon, and F. W. Luscinskas Elastase Release by Transmigrating Neutrophils Deactivates Endothelial-bound SDF-1{alpha} and Attenuates Subsequent T Lymphocyte Transendothelial Migration J. Exp. Med., September 20, 2004; 200(6): 713 - 724. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. H. Birdsall, W. J. Porter, D. M. Green, J. Rubio, J. Trial, and R. D. Rossen Impact of Fibronectin Fragments on the Transendothelial Migration of HIV-Infected Leukocytes and the Development of Subendothelial Foci of Infectious Leukocytes J. Immunol., August 15, 2004; 173(4): 2746 - 2754. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. da Costa Martins, N. van den Berk, L. H. Ulfman, L. Koenderman, P.L. Hordijk, and J.J. Zwaginga Platelet-Monocyte Complexes Support Monocyte Adhesion to Endothelium by Enhancing Secondary Tethering and Cluster Formation Arterioscler Thromb Vasc Biol, January 1, 2004; 24(1): 193 - 199. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Tchernychev, B. Furie, and B. C. Furie Peritoneal macrophages express both P-selectin and PSGL-1 J. Cell Biol., December 8, 2003; 163(5): 1145 - 1155. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Li, Y.-J. Kim, C. Mantel, and H. E. Broxmeyer P-Selectin Enhances Generation of CD14+CD16+ Dendritic-Like Cells and Inhibits Macrophage Maturation from Human Peripheral Blood Monocytes J. Immunol., July 15, 2003; 171(2): 669 - 677. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Sperandio, M. L. Smith, S. B. Forlow, T. S. Olson, L. Xia, R. P. McEver, and K. Ley P-selectin Glycoprotein Ligand-1 Mediates L-Selectin-dependent Leukocyte Rolling in Venules J. Exp. Med., May 19, 2003; 197(10): 1355 - 1363. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 O. J. T. McCarty, N. Tien, B. S. Bochner, and K. Konstantopoulos Exogenous eosinophil activation converts PSGL-1-dependent binding to CD18-dependent stable adhesion to platelets in shear flow Am J Physiol Cell Physiol, May 1, 2003; 284(5): C1223 - C1234. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Kanamori, N. Kojima, K. Uchimura, T. Muramatsu, T. Tamatani, M. C. Berndt, G. S. Kansas, and R. Kannagi Distinct Sulfation Requirements of Selectins Disclosed Using Cells That Support Rolling Mediated by All Three Selectins under Shear Flow. L-SELECTIN PREFERS CARBOHYDRATE 6-SULFATION TO TYROSINE SULFATION, WHEREAS P-SELECTIN DOES NOT J. Biol. Chem., August 30, 2002; 277(36): 32578 - 32586. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. A. Boyce, E. A. Mellor, B. Perkins, Y.-C. Lim, and F. W. Luscinskas Human mast cell progenitors use alpha 4-integrin, VCAM-1, and PSGL-1 E-selectin for adhesive interactions with human vascular endothelium under flow conditions Blood, April 15, 2002; 99(8): 2890 - 2896. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. K. Shaw, B. N. Perkins, Y.-C. Lim, Y. Liu, A. Nusrat, F. J. Schnell, C. A. Parkos, and F. W. Luscinskas Reduced Expression of Junctional Adhesion Molecule and Platelet/Endothelial Cell Adhesion Molecule-1 (CD31) at Human Vascular Endothelial Junctions by Cytokines Tumor Necrosis Factor-{alpha} Plus Interferon-{gamma} Does Not Reduce Leukocyte Transmigration Under Flow Am. J. Pathol., December 1, 2001; 159(6): 2281 - 2291. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. E. Eriksson, X. Xie, J. Werr, P. Thoren, and L. Lindbom Importance of Primary Capture and L-Selectin-Dependent Secondary Capture in Leukocyte Accumulation in Inflammation and Atherosclerosis in Vivo J. Exp. Med., July 16, 2001; 194(2): 205 - 218. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Hirata, G. Merrill-Skoloff, M. Aab, J. Yang, B. C. Furie, and B. Furie P-Selectin Glycoprotein Ligand 1 (Psgl-1) Is a Physiological Ligand for E-Selectin in Mediating T Helper 1 Lymphocyte Migration J. Exp. Med., December 4, 2000; 192(11): 1669 - 1676. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Nomura, F. Okamae, M. Abe, M. Hosokawa, M. Yamaoka, T. Ohtani, S. Onishi, T. Matsuzaki, A. Teraoka, T. Ishida, et al. Platelets Expressing P-Selectin and Platelet-Derived Microparticles in Stored Platelet Concentrates Bind to PSGL-1 on Filtrated Leukocytes Clinical and Applied Thrombosis/Hemostasis, October 1, 2000; 6(4): 213 - 221. [Abstract] [PDF]
|
|
|
|
|
|
D. W. Schmidtke and S. L. Diamond Direct Observation of Membrane Tethers Formed during Neutrophil Attachment to Platelets or P-Selectin under Physiological Flow J. Cell Biol., May 1, 2000; 149(3): 719 - 730. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. J. Mitchell, P. Li, P. H. Reinhardt, and P. Kubes Importance of L-selectin-dependent leukocyte-leukocyte interactions in human whole blood Blood, May 1, 2000; 95(9): 2954 - 2959. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. R. Allport, W. A. Muller, and F. W. Luscinskas Monocytes Induce Reversible Focal Changes in Vascular Endothelial Cadherin Complex during Transendothelial Migration under Flow J. Cell Biol., January 10, 2000; 148(1): 203 - 216. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Yago, M. Tsukuda, and M. Minami P-Selectin Binding Promotes the Adhesion of Monocytes to VCAM-1 Under Flow Conditions J. Immunol., July 1, 1999; 163(1): 367 - 373. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. S.C. Weber, G. Draude, W. Erl, R. de Martin, and C. Weber Monocyte Arrest and Transmigration on Inflamed Endothelium in Shear Flow Is Inhibited by Adenovirus-Mediated Gene Transfer of Ikappa B-alpha Blood, June 1, 1999; 93(11): 3685 - 3693. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. Dwir, Geoffrey. S. Kansas, and R. Alon An Activated L-selectin Mutant with Conserved Equilibrium Binding Properties but Enhanced Ligand Recognition under Shear Flow J. Biol. Chem., June 16, 2000; 275(25): 18682 - 18691. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
