| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
*
Department of Biomedical Engineering, University of Virginia School of Medicine, Charlottesville, VA 22908;
Department of Immunology, Duke University Medical Center, Durham, NC 27710; and
Institute for Genetics, University of Cologne, Cologne, Germany
Lymphocyte trafficking into Peyer’s patches requires ß7 integrins and L-selectin. Here, we use intravital microscopy to examine leukocyte rolling and adhesion in Peyer’s patch high endothelial venules (HEV) of wild-type, L-selectin-deficient (L-/-), ß7 integrin-deficient (ß7-/-), and ß7/L-/- mice. Although the leukocyte rolling flux fraction was reduced by 70%, Peyer’s patches in L-/- mice were of normal size and cellularity. In ß7-/- mice, the rolling flux fraction was normal, but the number of adherent leukocytes in HEV was greatly reduced. The median leukocyte rolling velocity was reduced in L-/- mice and increased in ß7-/- mice, suggesting that ß7 integrins and L-selectin mediate rolling in Peyer’s patch HEV at different velocities. ß7/L-/- exhibited both a low rolling flux fraction and low adhesion and had severely reduced Peyer’s patch size and cellularity. The residual rolling in these mice was completely blocked by a P-selectin mAb. A significant P-selectin component was also detected in the other genotypes. Twenty-six percent of B and T lymphocytes isolated from Peyer’s patches of wild-type mice expressed functional ligands for P-selectin, and this fraction was increased to 57% in ß7/L-/- mice. Peyer’s patch HEV were found to express P-selectin under the conditions of intravital microscopy, but not in situ. Our data suggest a novel P-selectin dependent mechanism of lymphocyte homing to Peyer’s patches. In situ, ß7 integrins and L-selectin account for all lymphocyte homing to Peyer’s patches, but P-selectin-dependent rolling, as induced by minimal trauma, may support trafficking of effector T lymphocytes to Peyer’s patches.
This article has been cited by other articles:
|
|
 |
|
  A. Martin-Fontecha, D. Baumjohann, G. Guarda, A. Reboldi, M. Hons, A. Lanzavecchia, and F. Sallusto CD40L+ CD4+ memory T cells migrate in a CD62P-dependent fashion into reactive lymph nodes and license dendritic cells for T cell priming J. Exp. Med., October 27, 2008; 205(11): 2561 - 2574. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. E. Eriksson No detectable endothelial- or leukocyte-derived L-selectin ligand activity on the endothelium in inflamed cremaster muscle venules J. Leukoc. Biol., July 1, 2008; 84(1): 93 - 103. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. V. Ostanin, K. L. Furr, K. P. Pavlick, L. Gray, C. G. Kevil, D. Shukla, D. D'Souza, J. M. Hoffman, and M. B. Grisham T cell-associated CD18 but not CD62L, ICAM-1, or PSGL-1 is required for the induction of chronic colitis Am J Physiol Gastrointest Liver Physiol, June 1, 2007; 292(6): G1706 - G1714. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Rydstrom and M. J. Wick Monocyte Recruitment, Activation, and Function in the Gut-Associated Lymphoid Tissue during Oral Salmonella Infection J. Immunol., May 1, 2007; 178(9): 5789 - 5801. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Harakawa, A. Shigeta, M. Wato, G. Merrill-Skoloff, B. C. Furie, B. Furie, T. Okazaki, N. Domae, M. Miyasaka, and T. Hirata P-selectin glycoprotein ligand-1 mediates L-selectin-independent leukocyte rolling in high endothelial venules of peripheral lymph nodes Int. Immunol., March 1, 2007; 19(3): 321 - 329. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Rivera-Nieves, T. L. Burcin, T. S. Olson, M. A. Morris, M. McDuffie, F. Cominelli, and K. Ley Critical role of endothelial P-selectin glycoprotein ligand 1 in chronic murine ileitis J. Exp. Med., April 17, 2006; 203(4): 907 - 917. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Zhou, Y. Luo, C. D. Kaplan, J. A. Kruger, S.-H. Lee, R. Xiang, and R. A. Reisfeld A DNA-based cancer vaccine enhances lymphocyte cross talk by engaging the NKG2D receptor Blood, April 15, 2006; 107(8): 3251 - 3257. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. R. Mangan, D. O'Quinn, L. Harrington, C. S. Bonder, P. Kubes, D. F. Kucik, D. C. Bullard, and C. T. Weaver Both Th1 and Th2 Cells Require P-Selectin Glycoprotein Ligand-1 for Optimal Rolling on Inflamed Endothelium Am. J. Pathol., December 1, 2005; 167(6): 1661 - 1675. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Rivera-Nieves, T. Olson, G. Bamias, A. Bruce, M. Solga, R. F. Knight, S. Hoang, F. Cominelli, and K. Ley L-Selectin, {alpha}4{beta}1, and {alpha}4{beta}7 Integrins Participate in CD4+ T Cell Recruitment to Chronically Inflamed Small Intestine J. Immunol., February 15, 2005; 174(4): 2343 - 2352. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Van Assche and P. Rutgeerts Physiological Basis for Novel Drug Therapies Used to Treat the Inflammatory Bowel Diseases I. Immunology and therapeutic potential of antiadhesion molecule therapy in inflammatory bowel disease Am J Physiol Gastrointest Liver Physiol, February 1, 2005; 288(2): G169 - G174. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Bistrup, D. Tsay, P. Shenoy, M. S. Singer, N. Bangia, S. A. Luther, J. G. Cyster, N. H. Ruddle, and S. D. Rosen Detection of a Sulfotransferase (HEC-GlcNAc6ST) in High Endothelial Venules of Lymph Nodes and in High Endothelial Venule-Like Vessels within Ectopic Lymphoid Aggregates: Relationship to the MECA-79 Epitope Am. J. Pathol., May 1, 2004; 164(5): 1635 - 1644. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Hiraoka, H. Kawashima, B. Petryniak, J. Nakayama, J. Mitoma, J. D. Marth, J. B. Lowe, and M. Fukuda Core 2 Branching {beta}1,6-N-Acetylglucosaminyltransferase and High Endothelial Venule-restricted Sulfotransferase Collaboratively Control Lymphocyte Homing J. Biol. Chem., January 23, 2004; 279(4): 3058 - 3067. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Ley Sulfated Sugars for Rolling Lymphocytes J. Exp. Med., November 3, 2003; 198(9): 1285 - 1288. [Full Text] [PDF]
|
|
|
|
|
|
A. van Zante, J.-M. Gauguet, A. Bistrup, D. Tsay, U. H. von Andrian, and S. D. Rosen Lymphocyte-HEV Interactions in Lymph Nodes of a Sulfotransferase-deficient Mouse J. Exp. Med., November 3, 2003; 198(9): 1289 - 1300. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. Haddad, C. J. Cooper, Z. Zhang, J. B. Brown, Y. Zhu, A. Issekutz, I. Fuss, H.-o. Lee, G. S. Kansas, and T. A. Barrett P-Selectin and P-Selectin Glycoprotein Ligand 1 Are Major Determinants for Th1 Cell Recruitment to Nonlymphoid Effector Sites in the Intestinal Lamina Propria J. Exp. Med., August 4, 2003; 198(3): 369 - 377. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. H. Grayson, R. S. Hotchkiss, I. E. Karl, M. J. Holtzman, and D. D. Chaplin Intravital microscopy comparing T lymphocyte trafficking to the spleen and the mesenteric lymph node Am J Physiol Heart Circ Physiol, June 1, 2003; 284(6): H2213 - H2226. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. M. Moore, W. B. Shirah, P. L. Khimenko, P. Paisley, R. N. Lausch, and A. E. Taylor Involvement of CD40-CD40L signaling in postischemic lung injury Am J Physiol Lung Cell Mol Physiol, December 1, 2002; 283(6): L1255 - L1262. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Redlin, J. Werner, H. Habazettl, W. Griethe, H. Kuppe, and A. R. Pries Cariporide (HOE 642) Attenuates Leukocyte Activation in Ischemia and Reperfusion Anesth. Analg., December 1, 2001; 93(6): 1472 - 1479. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Shigematsu, R. D. Specian, R. E. Wolf, M. B. Grisham, and D. N. Granger MAdCAM mediates lymphocyte-endothelial cell adhesion in a murine model of chronic colitis Am J Physiol Gastrointest Liver Physiol, November 1, 2001; 281(5): G1309 - G1315. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Sperandio, S. B. Forlow, J. Thatte, L. G. Ellies, J. D. Marth, and K. Ley Differential Requirements for Core2 Glucosaminyltransferase for Endothelial L-Selectin Ligand Function In Vivo J. Immunol., August 15, 2001; 167(4): 2268 - 2274. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Singbartl, J. Thatte, M. L. Smith, K. Wethmar, K. Day, and K. Ley A CD2-Green Fluorescence Protein-Transgenic Mouse Reveals Very Late Antigen-4-Dependent CD8+ Lymphocyte Rolling in Inflamed Venules J. Immunol., June 15, 2001; 166(12): 7520 - 7526. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 U. H. von Andrian and C. R. Mackay T-Cell Function and Migration -- Two Sides of the Same Coin N. Engl. J. Med., October 5, 2000; 343(14): 1020 - 1034. [Full Text] [PDF]
|
|
|
|
|
|
R.A. Warnock, J.J. Campbell, M.E. Dorf, A. Matsuzawa, L.M. McEvoy, and E.C. Butcher The Role of Chemokines in the Microenvironmental Control of T versus B Cell Arrest in Peyer's Patch High Endothelial Venules J. Exp. Med., January 3, 2000; 191(1): 77 - 88. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
U. Jung and K. Ley Mice Lacking Two or All Three Selectins Demonstrate Overlapping and Distinct Functions for Each Selectin J. Immunol., June 1, 1999; 162(11): 6755 - 6762. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. A. Steeber, M. L. K. Tang, X.-Q. Zhang, W. Muller, N. Wagner, and T. F. Tedder Efficient Lymphocyte Migration Across High Endothelial Venules of Mouse Peyer's Patches Requires Overlapping Expression of L-Selectin and {beta}7 Integrin J. Immunol., December 15, 1998; 161(12): 6638 - 6647. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
