| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
*
Terry Fox Laboratory, British Columbia Cancer Agency,
Department of Medical Genetics, and
Department of Pathology and Laboratory Medicine, University of British Columbia, Vancouver, British Columbia, Canada
The recognition of class I MHC molecules on target cells by the
Ly-49 family of receptors regulates NK cytotoxicity. Previous studies
have suggested that carbohydrates are involved in the recognition of
class I MHC by Ly-49, although their precise role remains unclear.
Here, we examined the role of asparagine-linked carbohydrates of the
murine class I MHC in the binding to Ly-49A and Ly-49C. We have
generated H-2Dd mutants that lack the highly conserved
glycosylation sites at amino acid residues 86 in the 
1 domain and
176 in the 2 domain, respectively. These mutant Dd cDNAs
were transfected into leukemic cell lines, and the binding of the
transfected cells to COS cells expressing Ly-49A or Ly-49C, as well as
their susceptibility to lysis by Ly-49A+ NK cells, was
examined. Only the mutation of the 2 domain glycosylation site
significantly reduced the binding of Dd to Ly-49A and
Ly-49C. Cells expressing Dd with the mutation at this site
were partially resistant to killing by Ly-49A+ NK cells.
These results suggest that, while carbohydrates linked to residue 176
seem to function as a part of the ligand structure for the Ly-49 family
of NK receptors, there are additional structural features involved in
this recognition. This glycosylation site is highly conserved among
murine class I MHC but is not found among those of other species,
suggesting that its role is unique to the murine immune system. It
further suggests that murine class I MHC and Ly-49 gene families may
have evolved in concert.
This article has been cited by other articles:
|
|
 |
|
  A. Martayan, L. Sibilio, A. Setini, E. L. Monaco, E. Tremante, D. Fruci, M. Colonna, and P. Giacomini N-Linked Glycosylation Selectively Regulates the Generic Folding of HLA-Cw1 J. Biol. Chem., June 13, 2008; 283(24): 16469 - 16476. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. A. Benoit and R. Tan Xenogeneic beta2-Microglobulin Substitution Alters NK Cell Function J. Immunol., August 1, 2007; 179(3): 1466 - 1474. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Maeda, C. Carpenito, R. C. Russell, J. Dasanjh, L. L. Veinotte, H. Ohta, T. Yamamura, R. Tan, and F. Takei Murine CD160, Ig-Like Receptor on NK Cells and NKT Cells, Recognizes Classical and Nonclassical MHC Class I and Regulates NK Cell Activation J. Immunol., October 1, 2005; 175(7): 4426 - 4432. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Maeda, A. Shadeo, A. M. MacFadyen, and F. Takei CD1d-Independent NKT Cells in {beta}2-Microglobulin-Deficient Mice Have Hybrid Phenotype and Function of NK and T Cells J. Immunol., May 15, 2004; 172(10): 6115 - 6122. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. S. J. Marshall, J. A. Willment, H.-H. Lin, D. L. Williams, S. Gordon, and G. D. Brown Identification and Characterization of a Novel Human Myeloid Inhibitory C-type Lectin-like Receptor (MICL) That Is Predominantly Expressed on Granulocytes and Monocytes J. Biol. Chem., April 9, 2004; 279(15): 14792 - 14802. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. H. Mason, J. Willette-Brown, S. K. Anderson, W. G. Alvord, R. L. Klabansky, H. A. Young, and J. R. Ortaldo Receptor Glycosylation Regulates Ly-49 Binding to MHC Class I J. Immunol., October 15, 2003; 171(8): 4235 - 4242. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. H. Lian, M. Maeda, S. Lohwasser, M. Delcommenne, T. Nakano, R. E. Vance, D. H. Raulet, and F. Takei Orderly and Nonstochastic Acquisition of CD94/NKG2 Receptors by Developing NK Cells Derived from Embryonic Stem Cells In Vitro J. Immunol., May 15, 2002; 168(10): 4980 - 4987. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Sundback, A. Achour, J. Michaelsson, H. Lindstrom, and K. Karre NK Cell Inhibitory Receptor Ly-49C Residues Involved in MHC Class I Binding J. Immunol., January 15, 2002; 168(2): 793 - 800. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. A. Willment, S. Gordon, and G. D. Brown Characterization of the Human beta -Glucan Receptor and Its Alternatively Spliced Isoforms J. Biol. Chem., November 16, 2001; 276(47): 43818 - 43823. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Matsumoto, M. Mitsuki, K. Tajima, W. M. Yokoyama, and K. Yamamoto The Functional Binding Site for the C-type Lectin-like Natural Killer Cell Receptor Ly49A Spans Three Domains of Its Major Histocompatibility Complex Class I Ligand J. Exp. Med., January 8, 2001; 193(2): 147 - 158. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. A. Tripp, D. Moore, L. Jones, W. Sullender, J. Winter, and L. J. Anderson Respiratory Syncytial Virus G and/or SH Protein Alters Th1 Cytokines, Natural Killer Cells, and Neutrophils Responding to Pulmonary Infection in BALB/c Mice J. Virol., September 1, 1999; 73(9): 7099 - 7107. [Abstract] [Full Text]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
