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MHC Class II Transport from Lysosomal Compartments to the Cell Surface Is Determined by Stable Peptide Binding, But Not by the Cytosolic Domains of the - and ß-Chains¹

Clotilde Théry^*, Valérie Brachet^*, Armelle Regnault^*, Maria Rescigno, Paola Ricciardi-Castagnoli, Christian Bonnerot^* and Sebastian Amigorena^2,*

^* Institut National de la Santé et de la Recherche Médicale, CJF 95-01, Institut Curie, Section Recherche, Paris, France; and Consiglio Nazionale delle Ricerche, Center of Cellular and Molecular Pharmacology, Milan, Italy

Inside APCs, MHC class II molecules associate with antigenic peptides before reaching the cell surface. This association takes place in compartments of the endocytic pathway, more related to endosomes or lysosomes depending on the cell type. Here, we compared MHC class II transport from endosomal vs lysosomal compartments to the plasma membrane. We show that transport of MHC class II molecules to the cell surface does not depend on the cytosolic domains of the - and ß-chains. In contrast, the stability of the ß-peptide complexes determined the efficiency of transport to the cell surface from lysosomal, but not from endosomal, compartments. In murine B lymphoma cells, SDS-unstable and -stable complexes were transported to the cell surface at almost similar rates, whereas after lysosomal relocalization or in a cell line in which MHC class II molecules normally accumulate in lysosomal compartments, stable complexes were preferentially addressed to the cell surface. Our results suggest that when peptide loading occurs in lysosomal compartments, selective retention and lysosomal degradation of unstable dimers result in the expression of highly stable MHC class II-peptide complexes at the APC surface.

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