HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Demaison, C. Articles by Froussard, P. Search for Related Content PubMed PubMed Citation Articles by Demaison, C. Articles by Froussard, P.

IL-2 Receptor -Chain Expression Is Independently Regulated in Primary and Secondary Lymphoid Organs

Christophe Demaison^1,*, Laurence Fiette, Valérie Blanchetière^*, Anneliese Schimpl, Jacques Thèze^2,* and P. Froussard^*

^* Unité d’Immunogénétique Cellulaire and Unité d’Histopathologie, Institut Pasteur, Paris, France; and Institute of Virology and Immunobiology, University of Würzburg, Würzburg, Germany

The IL-2R is composed of three chains: IL-2R, IL-2Rß, and IL-2R. In mice, IL-2R is critical and determines IL-2 binding to the tripartite IL-2R complex. To extend our previous studies, which demonstrated that IL-2 regulates IL-2R expression in vitro, we have analyzed expression in IL-2-deficient mice in vivo. As in control animals, CD4^-CD8^- thymocytes and bone marrow-derived B220⁺ pre-B cells were Il-2R positive. In contrast, activated lymph node and splenic CD4 T cells (CD4⁺CD69⁺) were found to be IL-2R negative, whereas 20% of the same cell populations from the MLR/lpr strain, which also accumulate large numbers of CD4-activated T cells in the presence of intact IL-2, retained expression. A similar pattern of IL-2R expression was found among splenic CD8 cells from IL-2^-/- and IL-2^+/- animals. These findings demonstrate that in primary lymphoid organs, IL-2 is not directly involved in IL-2R expression. However, at the level of mature lymphocytes, and more specifically CD4 T cells, IL-2 remains in vivo, as in vitro, the most critical cytokine controlling both IL-2R expression and sensitivity to IL-2.

This article has been cited by other articles:

				T. Vowinkel, K. C. Wood, K. Y. Stokes, J. Russell, C. F. Krieglstein, and D. N. Granger Differential expression and regulation of murine CD40 in regional vascular beds Am J Physiol Heart Circ Physiol, February 1, 2006; 290(2): H631 - H639. [Abstract] [Full Text] [PDF]

				F. Gesbert, J.-L. Moreau, and J. Theze IL-2 Responsiveness of CD4 and CD8 lymphocytes: further investigations with human IL-2R{beta} transgenic mice Int. Immunol., August 1, 2005; 17(8): 1093 - 1102. [Abstract] [Full Text] [PDF]

				J. Valenzuela, C. Schmidt, and M. Mescher The Roles of IL-12 in Providing a Third Signal for Clonal Expansion of Naive CD8 T Cells J. Immunol., December 15, 2002; 169(12): 6842 - 6849. [Abstract] [Full Text] [PDF]

				P. Chastagner, J. Reddy, and J. Theze Lymphoadenopathy in IL-2-Deficient Mice: Further Characterization and Overexpression of the Antiapoptotic Molecule Cellular FLIP J. Immunol., October 1, 2002; 169(7): 3644 - 3651. [Abstract] [Full Text] [PDF]

				J.-H. Yeh, S. Spicuglia, S. Kumar, A. Sanchez-Sevilla, P. Ferrier, and J. Imbert Control of IL-2R{alpha} gene expression: structural changes within the proximal enhancer/core promoter during T-cell development Nucleic Acids Res., May 1, 2002; 30(9): 1944 - 1951. [Abstract] [Full Text] [PDF]

				J. Reddy, P. Chastagner, L. Fiette, X. Liu, and J. Theze IL-2-induced tumor necrosis factor (TNF)-{beta} expression: further analysis in the IL-2 knockout model, and comparison with TNF-{{alpha}}, lymphotoxin-{beta}, TNFR1 and TNFR2 modulation Int. Immunol., February 1, 2001; 13(2): 135 - 147. [Abstract] [Full Text] [PDF]