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IL-2 Receptor -Chain Expression Is Independently Regulated in Primary and Secondary Lymphoid Organs

Christophe Demaison^1,*, Laurence Fiette, Valérie Blanchetière^*, Anneliese Schimpl, Jacques Thèze^2,* and P. Froussard^*

^* Unité d’Immunogénétique Cellulaire and Unité d’Histopathologie, Institut Pasteur, Paris, France; and Institute of Virology and Immunobiology, University of Würzburg, Würzburg, Germany

The IL-2R is composed of three chains: IL-2R, IL-2Rß, and IL-2R. In mice, IL-2R is critical and determines IL-2 binding to the tripartite IL-2R complex. To extend our previous studies, which demonstrated that IL-2 regulates IL-2R expression in vitro, we have analyzed expression in IL-2-deficient mice in vivo. As in control animals, CD4^-CD8^- thymocytes and bone marrow-derived B220⁺ pre-B cells were Il-2R positive. In contrast, activated lymph node and splenic CD4 T cells (CD4⁺CD69⁺) were found to be IL-2R negative, whereas 20% of the same cell populations from the MLR/lpr strain, which also accumulate large numbers of CD4-activated T cells in the presence of intact IL-2, retained expression. A similar pattern of IL-2R expression was found among splenic CD8 cells from IL-2^-/- and IL-2^+/- animals. These findings demonstrate that in primary lymphoid organs, IL-2 is not directly involved in IL-2R expression. However, at the level of mature lymphocytes, and more specifically CD4 T cells, IL-2 remains in vivo, as in vitro, the most critical cytokine controlling both IL-2R expression and sensitivity to IL-2.

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