The JI
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     
 

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Request Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Aguirre, S. A.
Right arrow Articles by McGuire, T. C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Aguirre, S. A.
Right arrow Articles by McGuire, T. C.
The Journal of Immunology, 1998, 161: 1891-1900.
Copyright © 1998 by The American Association of Immunologists

IL-4 Protects Adult C57BL/6 Mice from Prolonged Cryptosporidium parvum Infection: Analysis of CD4+{alpha}ß+IFN-{gamma}+ and CD4+{alpha}ß+IL-4+ Lymphocytes in Gut-Associated Lymphoid Tissue During Resolution of Infection1

Shirley A. Aguirre2, Lance E. Perryman{dagger}, William C. Davis* and Travis C. McGuire*

* Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164; and {dagger} Department of Microbiology, Pathology, and Parasitology, North Carolina State University College of Veterinary Medicine, Raleigh, NC 27606

Resistance of adult C57BL/6 mice to severe Cryptosporidium parvum infection is dependent on CD4+{alpha}ß+ TCR lymphocytes. In this study, we demonstrated that treatment with anti-IFN-{gamma} mAb extended oocyst excretion 18 days longer, and anti-IL-4 mAb extended oocyst excretion at least 11 days longer than isotype control mAb treatment. Analysis of the specific activity of anti-IFN-{gamma} mAb present in treated mouse sera suggested that IFN-{gamma} may have a limited role in the resolution phase of infection. Changes were also documented in numbers of CD4+{alpha}ß+IFN-{gamma}+ and CD4+{alpha}ß+IL-4+ lymphocytes in Peyer’s patches and intraepithelium of adult C57BL/6 mice during resolution of C. parvum infection. Resistance to initial severe infection was associated with CD4+{alpha}ß+IFN-{gamma}+ lymphocytes, and eventual resolution of infection was associated with CD4+{alpha}ß+IL-4+ lymphocytes. Analysis of cytokine expression following in vitro stimulation with C. parvum Ags during resolution of infection demonstrated consistent increases in CD4+{alpha}ß+IL-4+ lymphocytes, but not CD4+{alpha}ß+IFN-{gamma}+ lymphocytes. The relevance of CD4+{alpha}ß+IL-4+ lymphocytes in protection against C. parvum was then evaluated in C57BL/6 IL-4 gene knockout mice (IL-4-/-). Adult IL-4-/- mice excreted oocysts in feces approximately 23 days longer than IL-4+/+ mice. Further, anti-IFN-{gamma} mAb treatment increased the severity and the duration of infection in IL-4-/- mice compared with those in IL-4+/+ mice. Together, the data demonstrated that IFN-{gamma} was important in the control of severity of infection, and either IFN-{gamma} or IL-4 accelerated termination of infection. However, neither IL-4 nor IFN-{gamma} was required for the final clearance of infection from the intestinal tract of adult mice.




This article has been cited by other articles:


Home page
 BloodHome page
S. Noda, S. A. Aguirre, A. Bitmansour, J. M. Brown, T. E. Sparer, J. Huang, and E. S. Mocarski
Cytomegalovirus MCK-2 controls mobilization and recruitment of myeloid progenitor cells to facilitate dissemination
Blood, January 1, 2006; 107(1): 30 - 38.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
I.-S. Lean, S. A. C. McDonald, M. Bajaj-Elliott, R. C. G. Pollok, M. J. G. Farthing, and V. McDonald
Interleukin-4 and Transforming Growth Factor {beta} Have Opposing Regulatory Effects on Gamma Interferon-Mediated Inhibition of Cryptosporidium parvum Reproduction
Infect. Immun., August 1, 2003; 71(8): 4580 - 4585.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
S. Lacroix, R. Mancassola, M. Naciri, and F. Laurent
Cryptosporidium parvum-Specific Mucosal Immune Response in C57BL/6 Neonatal and Gamma Interferon-Deficient Mice: Role of Tumor Necrosis Factor Alpha in Protection
Infect. Immun., March 1, 2001; 69(3): 1635 - 1642.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
J. K. Roche, C. A. P. Martins, R. Cosme, R. Fayer, and R. L. Guerrant
Transforming Growth Factor beta 1 Ameliorates Intestinal Epithelial Barrier Disruption by Cryptosporidium parvum In Vitro in the Absence of Mucosal T Lymphocytes
Infect. Immun., October 1, 2000; 68(10): 5635 - 5644.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
K. G.-E. Scott, M. R. Logan, G. M. Klammer, D. A. Teoh, and A. G. Buret
Jejunal Brush Border Microvillous Alterations in Giardia muris-Infected Mice: Role of T Lymphocytes and Interleukin-6
Infect. Immun., June 1, 2000; 68(6): 3412 - 3418.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
K. Lukin, M. Cosyns, T. Mitchell, M. Saffry, and A. Hayward
Eradication of Cryptosporidium parvum Infection by Mice with Ovalbumin-Specific T Cells
Infect. Immun., May 1, 2000; 68(5): 2663 - 2670.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
G. J. Leitch and Q. He
Reactive Nitrogen and Oxygen Species Ameliorate Experimental Cryptosporidiosis in the Neonatal BALB/c Mouse Model
Infect. Immun., November 1, 1999; 67(11): 5885 - 5891.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Website Copyright © 1998 by The American Association of Immunologists, Inc. All rights reserved.
All Contents Copyright © 1998 by The American Association of Immunologists, Inc. All rights reserved.