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The Journal of Immunology, 1998, 161: 1399-1405.
Copyright © 1998 by The American Association of Immunologists

A Developmental Stage-Specific Promoter Directs Germline Transcription of DßJß Gene Segments in Precursor T Lymphocytes1

Michael L. Sikes, Rey J. Gomez, Jing Song and Eugene M. Oltz2

Department of Microbiology and Immunology, Vanderbilt University Medical School, Nashville, TN 37232

The tissue- and stage-specific assembly of Ag receptor genes is regulated by transcriptional control elements positioned within Ig and TCR loci. To further understand the role of cis-acting elements in these regulatory mechanisms, we have characterized a transcriptional promoter that drives germline expression of TCRß gene segments in vivo. The activity of this promoter, termed PDß, is restricted to a highly conserved 400-bp region located directly upstream from Dß1-coding sequences. Maximal PDß activity requires a TATA element situated within the Dß1 recombination signal sequences and consensus binding sites for the ubiquitous SP1 and the T cell-specific GATA-3 transcription factors. When linked to active enhancer elements, PDß directs transcription in most cell types; however, the TCRß enhancer (Eß) stimulates PDß function specifically in precursor T lymphocytes. These findings suggest that PDß/Eß interactions may contribute to differential regulation of regions within the TCRß locus during thymocyte development.




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