HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Wykes, M. Articles by MacPherson, G. G. Search for Related Content PubMed PubMed Citation Articles by Wykes, M. Articles by MacPherson, G. G. Pubmed/NCBI databases Compound via MeSH Substance via MeSH

Dendritic Cells Interact Directly with Naive B Lymphocytes to Transfer Antigen and Initiate Class Switching in a Primary T-Dependent Response¹

Sir William Dunn School of Pathology, University of Oxford, Oxford, United Kingdom

Dendritic cells (DC) are thought to initiate Ab synthesis by activation of T cells, which then provide cytokine and cell-bound "help" to B cells. Here, we provide evidence that DC can capture and retain unprocessed Ag in vitro and in vivo, and can transfer this Ag to naive B cells to initiate a specific Ab response. The response is skewed with 4- to 13-fold higher titers of IgG than IgM, and the predominant subclasses of Ab produced in naive animals are those associated with Th2-type responses. Ag retention and the skew in class switching is a physiologic phenomenon because DC loaded with Ag in vivo and isolated 24 h later initiated a class-switched, Ag-specific Ab response in naive animals. In vitro studies confirmed that DC provide naive B cells with signals that are essential for the synthesis of class-switched Ab. Taken together, these observations show that DC have an important role in the initiation of Ab synthesis by direct interaction with B cells.

This article has been cited by other articles:

				L. Fahlen-Yrlid, T. Gustafsson, J. Westlund, A. Holmberg, A. Strombeck, M. Blomquist, G. G. MacPherson, J. Holmgren, and U. Yrlid CD11chigh Dendritic Cells Are Essential for Activation of CD4+ T Cells and Generation of Specific Antibodies following Mucosal Immunization J. Immunol., October 15, 2009; 183(8): 5032 - 5041. [Abstract] [Full Text] [PDF]

				E. Y. Enioutina, D. Bareyan, and R. A. Daynes TLR-Induced Local Metabolism of Vitamin D3 Plays an Important Role in the Diversification of Adaptive Immune Responses J. Immunol., April 1, 2009; 182(7): 4296 - 4305. [Abstract] [Full Text] [PDF]

				D. Depoil, M. Weber, B. Treanor, S. J. Fleire, Y. R. Carrasco, N. E. Harwood, and F. D. Batista Early Events of B Cell Activation by Antigen Sci. Signal., March 24, 2009; 2(63): pt1 - pt1. [Abstract] [Full Text] [PDF]

				I. Douagi, C. Gujer, C. Sundling, W. C. Adams, A. Smed-Sorensen, R. A. Seder, G. B. Karlsson Hedestam, and K. Lore Human B Cell Responses to TLR Ligands Are Differentially Modulated by Myeloid and Plasmacytoid Dendritic Cells J. Immunol., February 15, 2009; 182(4): 1991 - 2001. [Abstract] [Full Text] [PDF]

				G. Chattopadhyay, Q. Chen, J. Colino, A. Lees, and C. M. Snapper Intact Bacteria Inhibit the Induction of Humoral Immune Responses to Bacterial-Derived and Heterologous Soluble T Cell-Dependent Antigens J. Immunol., February 15, 2009; 182(4): 2011 - 2019. [Abstract] [Full Text] [PDF]

				M. McPherson, B. Wei, O. Turovskaya, D. Fujiwara, S. Brewer, and J. Braun Colitis immunoregulation by CD8+ T cell requires T cell cytotoxicity and B cell peptide antigen presentation Am J Physiol Gastrointest Liver Physiol, September 1, 2008; 295(3): G485 - G492. [Abstract] [Full Text] [PDF]

				E. Arana, N. E. Harwood, and F. D. Batista Regulation of integrin activation through the B-cell receptor J. Cell Sci., July 15, 2008; 121(14): 2279 - 2286. [Abstract] [Full Text] [PDF]

				T. L. McGaha, M. C. I. Karlsson, and J. V. Ravetch Fc{gamma}RIIB Deficiency Leads to Autoimmunity and a Defective Response to Apoptosis in Mrl-MpJ Mice J. Immunol., April 15, 2008; 180(8): 5670 - 5679. [Abstract] [Full Text] [PDF]

				M. Weber, B. Treanor, D. Depoil, H. Shinohara, N. E. Harwood, M. Hikida, T. Kurosaki, and F. D. Batista Phospholipase C-{gamma}2 and Vav cooperate within signaling microclusters to propagate B cell spreading in response to membrane-bound antigen J. Exp. Med., April 14, 2008; 205(4): 853 - 868. [Abstract] [Full Text] [PDF]

				L. Santos, K. E. Draves, M. Boton, P. K. Grewal, J. D. Marth, and E. A. Clark Dendritic Cell-Dependent Inhibition of B Cell Proliferation Requires CD22 J. Immunol., April 1, 2008; 180(7): 4561 - 4569. [Abstract] [Full Text] [PDF]

				C. Ronet, H. Voigt, H. Himmelrich, M.-A. Doucey, Y. Hauyon-La Torre, M. Revaz-Breton, F. Tacchini-Cottier, C. Bron, J. Louis, and P. Launois Leishmania major-Specific B Cells Are Necessary for Th2 Cell Development and Susceptibility to L. major LV39 in BALB/c Mice J. Immunol., April 1, 2008; 180(7): 4825 - 4835. [Abstract] [Full Text] [PDF]

				C. R. Raymond, P. Aucouturier, and N. A. Mabbott In Vivo Depletion of CD11c+ Cells Impairs Scrapie Agent Neuroinvasion from the Intestine J. Immunol., December 1, 2007; 179(11): 7758 - 7766. [Abstract] [Full Text] [PDF]

				C. Hansell, X. W. Zhu, H. Brooks, M. Sheppard, S. Withanage, D. Maskell, and I. McConnell Unique Features and Distribution of the Chicken CD83+ Cell J. Immunol., October 15, 2007; 179(8): 5117 - 5125. [Abstract] [Full Text] [PDF]

				M. N. Wykes, X. Q. Liu, S. Jiang, C. Hirunpetcharat, and M. F. Good Systemic Tumor Necrosis Factor Generated during Lethal Plasmodium Infections Impairs Dendritic Cell Function J. Immunol., September 15, 2007; 179(6): 3982 - 3987. [Abstract] [Full Text] [PDF]

				M. Ostrowski, M. Vermeulen, O. Zabal, P. I. Zamorano, A. M. Sadir, J. R. Geffner, and O. J. Lopez The Early Protective Thymus-Independent Antibody Response to Foot-and-Mouth Disease Virus Is Mediated by Splenic CD9+ B Lymphocytes J. Virol., September 1, 2007; 81(17): 9357 - 9367. [Abstract] [Full Text] [PDF]

				R. Fang, N. Ismail, L. Soong, V. L. Popov, T. Whitworth, D. H. Bouyer, and D. H. Walker Differential Interaction of Dendritic Cells with Rickettsia conorii: Impact on Host Susceptibility to Murine Spotted Fever Rickettsiosis Infect. Immun., June 1, 2007; 75(6): 3112 - 3123. [Abstract] [Full Text] [PDF]

				E. Scandella, K. Fink, T. Junt, B. M. Senn, E. Lattmann, R. Forster, H. Hengartner, and B. Ludewig Dendritic Cell-Independent B Cell Activation During Acute Virus Infection: A Role for Early CCR7-Driven B-T Helper Cell Collaboration J. Immunol., February 1, 2007; 178(3): 1468 - 1476. [Abstract] [Full Text] [PDF]

				M. Chieppa, M. Rescigno, A. Y.C. Huang, and R. N. Germain Dynamic imaging of dendritic cell extension into the small bowel lumen in response to epithelial cell TLR engagement J. Exp. Med., December 25, 2006; 203(13): 2841 - 2852. [Abstract] [Full Text] [PDF]

				L. Delamarre, R. Couture, I. Mellman, and E. S. Trombetta Enhancing immunogenicity by limiting susceptibility to lysosomal proteolysis J. Exp. Med., September 4, 2006; 203(9): 2049 - 2055. [Abstract] [Full Text] [PDF]

				A. Seamons, A. Perchellet, and J. Goverman Endogenous Myelin Basic Protein Is Presented in the Periphery by Both Dendritic Cells and Resting B Cells with Different Functional Consequences J. Immunol., August 15, 2006; 177(4): 2097 - 2106. [Abstract] [Full Text] [PDF]

				H. Qi, J. G. Egen, A. Y. C. Huang, and R. N. Germain Extrafollicular activation of lymph node B cells by antigen-bearing dendritic cells. Science, June 16, 2006; 312(5780): 1672 - 1676. [Abstract] [Full Text] [PDF]

				S. J. Fleire, J. P. Goldman, Y. R. Carrasco, M. Weber, D. Bray, and F. D. Batista B cell ligand discrimination through a spreading and contraction response. Science, May 5, 2006; 312(5774): 738 - 741. [Abstract] [Full Text] [PDF]

				N.-N. Huang, S.-B. Han, I.-Y. Hwang, and J. H. Kehrl B Cells Productively Engage Soluble Antigen-Pulsed Dendritic Cells: Visualization of Live-Cell Dynamics of B Cell-Dendritic Cell Interactions J. Immunol., December 1, 2005; 175(11): 7125 - 7134. [Abstract] [Full Text] [PDF]

				C. von Garnier, L. Filgueira, M. Wikstrom, M. Smith, J. A. Thomas, D. H. Strickland, P. G. Holt, and P. A. Stumbles Anatomical Location Determines the Distribution and Function of Dendritic Cells and Other APCs in the Respiratory Tract J. Immunol., August 1, 2005; 175(3): 1609 - 1618. [Abstract] [Full Text] [PDF]

				P. Vanlandschoot, F. Van Houtte, P. Ulrichts, J. Tavernier, and G. Leroux-Roels Immunostimulatory potential of hepatitis B nucleocapsid preparations: lipopolysaccharide contamination should not be overlooked J. Gen. Virol., February 1, 2005; 86(2): 323 - 331. [Abstract] [Full Text] [PDF]

				J. Mohan, M. E. Bruce, and N. A. Mabbott Neuroinvasion by Scrapie following Inoculation via the Skin Is Independent of Migratory Langerhans Cells J. Virol., February 1, 2005; 79(3): 1888 - 1897. [Abstract] [Full Text] [PDF]

				E. G. Cormier, R. J. Durso, F. Tsamis, L. Boussemart, C. Manix, W. C. Olson, J. P. Gardner, and T. Dragic L-SIGN (CD209L) and DC-SIGN (CD209) mediate transinfection of liver cells by hepatitis C virus PNAS, September 28, 2004; 101(39): 14067 - 14072. [Abstract] [Full Text] [PDF]

				M. Vermeulen, M. Giordano, A. S. Trevani, C. Sedlik, R. Gamberale, P. Fernandez-Calotti, G. Salamone, S. Raiden, J. Sanjurjo, and J. R. Geffner Acidosis Improves Uptake of Antigens and MHC Class I-Restricted Presentation by Dendritic Cells J. Immunol., March 1, 2004; 172(5): 3196 - 3204. [Abstract] [Full Text] [PDF]

				J. Poudrier, X. Weng, D. G. Kay, Z. Hanna, and P. Jolicoeur The AIDS-Like Disease of CD4C/Human Immunodeficiency Virus Transgenic Mice Is Associated with Accumulation of Immature CD11bHi Dendritic Cells J. Virol., November 1, 2003; 77(21): 11733 - 11744. [Abstract] [Full Text] [PDF]

				M. Y. Sangster, J. M. Riberdy, M. Gonzalez, D. J. Topham, N. Baumgarth, and P. C. Doherty An Early CD4+ T Cell-dependent Immunoglobulin A Response to Influenza Infection in the Absence of Key Cognate T-B Interactions J. Exp. Med., October 6, 2003; 198(7): 1011 - 1021. [Abstract] [Full Text] [PDF]

				L. Gorelik, K. Gilbride, M. Dobles, S. L. Kalled, D. Zandman, and M. L. Scott Normal B Cell Homeostasis Requires B Cell Activation Factor Production by Radiation-resistant Cells J. Exp. Med., September 15, 2003; 198(6): 937 - 945. [Abstract] [Full Text] [PDF]

				H. Shiono, Y. L. Wong, I. Matthews, J.-L. Liu, W. Zhang, G. Sims, A. Meager, D. Beeson, A. Vincent, and N. Willcox Spontaneous production of anti-IFN-{alpha} and anti-IL-12 autoantibodies by thymoma cells from myasthenia gravis patients suggests autoimmunization in the tumor Int. Immunol., August 1, 2003; 15(8): 903 - 913. [Abstract] [Full Text] [PDF]

				M. Gilliet, M. Kleinhans, E. Lantelme, D. Schadendorf, G. Burg, and F. O. Nestle Intranodal injection of semimature monocyte-derived dendritic cells induces T helper type 1 responses to protein neoantigen Blood, July 1, 2003; 102(1): 36 - 42. [Abstract] [Full Text] [PDF]

				T. Yamanaka, L. Helgeland, I. N. Farstad, H. Fukushima, T. Midtvedt, and P. Brandtzaeg Microbial Colonization Drives Lymphocyte Accumulation and Differentiation in the Follicle-Associated Epithelium of Peyer's Patches J. Immunol., January 15, 2003; 170(2): 816 - 822. [Abstract] [Full Text] [PDF]

				S. Manoj, P. J. Griebel, L. A. Babiuk, and S. van Drunen Littel-van den Hurk Targeting with Bovine CD154 Enhances Humoral Immune Responses Induced by a DNA Vaccine in Sheep J. Immunol., January 15, 2003; 170(2): 989 - 996. [Abstract] [Full Text] [PDF]

				V. K. Shreedhar, B. L. Kelsall, and M. R. Neutra Cholera Toxin Induces Migration of Dendritic Cells from the Subepithelial Dome Region to T- and B-Cell Areas of Peyer's Patches Infect. Immun., January 1, 2003; 71(1): 504 - 509. [Abstract] [Full Text] [PDF]

				B. Chackerian, P. Lenz, D. R. Lowy, and J. T. Schiller Determinants of Autoantibody Induction by Conjugated Papillomavirus Virus-Like Particles J. Immunol., December 1, 2002; 169(11): 6120 - 6126. [Abstract] [Full Text] [PDF]

				S. G. Tangye, A. Ferguson, D. T. Avery, C. S. Ma, and P. D. Hodgkin Isotype Switching by Human B Cells Is Division-Associated and Regulated by Cytokines J. Immunol., October 15, 2002; 169(8): 4298 - 4306. [Abstract] [Full Text] [PDF]

				M. Wykes, K. P. A. MacDonald, M. Tran, R. J. Quin, P. X. Xing, S. J. Gendler, D. N. J. Hart, and M. A. McGuckin MUC1 epithelial mucin (CD227) is expressed by activated dendritic cells J. Leukoc. Biol., October 1, 2002; 72(4): 692 - 701. [Abstract] [Full Text] [PDF]

				M. Lindstedt, B. Johansson-Lindbom, and C. A. K. Borrebaeck Global reprogramming of dendritic cells in response to a concerted action of inflammatory mediators Int. Immunol., October 1, 2002; 14(10): 1203 - 1213. [Abstract] [Full Text] [PDF]

				S. Hirohata, T. Yanagida, T. Tomita, H. Yoshikawa, and T. Ochi Bone marrow CD34+ progenitor cells stimulated with stem cell factor and GM-CSF have the capacity to activate IgD- B cells through direct cellular interaction J. Leukoc. Biol., June 1, 2002; 71(6): 987 - 995. [Abstract] [Full Text] [PDF]

				M.-T. Wu and S. T. Hwang CXCR5-Transduced Bone Marrow-Derived Dendritic Cells Traffic to B Cell Zones of Lymph Nodes and Modify Antigen-Specific Immune Responses J. Immunol., May 15, 2002; 168(10): 5096 - 5102. [Abstract] [Full Text] [PDF]

				P. Yu, Y. Wang, R. K. Chin, L. Martinez-Pomares, S. Gordon, M. H. Kosco-Vibois, J. Cyster, and Y.-X. Fu B Cells Control the Migration of a Subset of Dendritic Cells into B Cell Follicles Via CXC Chemokine Ligand 13 in a Lymphotoxin-Dependent Fashion J. Immunol., May 15, 2002; 168(10): 5117 - 5123. [Abstract] [Full Text] [PDF]

				P. S. Rajapakse, N. M. O'Brien-Simpson, N. Slakeski, B. Hoffmann, and E. C. Reynolds Immunization with the RgpA-Kgp Proteinase-Adhesin Complexes of Porphyromonas gingivalis Protects against Periodontal Bone Loss in the Rat Periodontitis Model Infect. Immun., May 1, 2002; 70(5): 2480 - 2486. [Abstract] [Full Text] [PDF]

				F.-P. Huang, C. F. Farquhar, N. A. Mabbott, M. E. Bruce, and G. G. MacPherson Migrating intestinal dendritic cells transport PrPSc from the gut J. Gen. Virol., January 1, 2002; 83(1): 267 - 271. [Abstract] [Full Text] [PDF]

				M. F. Lipscomb and B. J. Masten Dendritic Cells: Immune Regulators in Health and Disease Physiol Rev, January 1, 2002; 82(1): 97 - 130. [Abstract] [Full Text] [PDF]

				J. Colino, Y. Shen, and C. M. Snapper Dendritic Cells Pulsed with Intact Streptococcus pneumoniae Elicit both Protein- and Polysaccharide-specific Immunoglobulin Isotype Responses In Vivo through Distinct Mechanisms J. Exp. Med., December 31, 2001; 195(1): 1 - 14. [Abstract] [Full Text] [PDF]

				J.-Y. Wu, R. K. Taylor, and W. F. Wade Anti-Class II Monoclonal Antibody-Targeted Vibrio cholerae TcpA Pilin: Modulation of Serologic Response, Epitope Specificity, and Isotype Infect. Immun., December 1, 2001; 69(12): 7679 - 7686. [Abstract] [Full Text] [PDF]

				B. A. Cocca, S. N. Seal, P. D'Agnillo, Y. M. Mueller, P. D. Katsikis, J. Rauch, M. Weigert, and M. Z. Radic Structural basis for autoantibody recognition of phosphatidylserine-beta 2 glycoprotein I and apoptotic cells PNAS, November 20, 2001; 98(24): 13826 - 13831. [Abstract] [Full Text] [PDF]

				C. G. F. Mueller, I. Cremer, P. E. Paulet, S. Niida, N. Maeda, S. Lebeque, W. H. Fridman, and C. Sautes-Fridman Mannose Receptor Ligand-Positive Cells Express the Metalloprotease Decysin in the B Cell Follicle J. Immunol., November 1, 2001; 167(9): 5052 - 5060. [Abstract] [Full Text] [PDF]

				E. M. Ponnuraj and A. R. Hayward Intact Intestinal mRNAs and Intestinal Epithelial Cell Esterase, But Not Cryptosporidium parvum, Reach Mesenteric Lymph Nodes of Infected Mice J. Immunol., November 1, 2001; 167(9): 5321 - 5328. [Abstract] [Full Text] [PDF]

				B.N. Lambrecht, J-;B. Prins, and H.C. Hoogsteden Lung dendritic cells and host immunity to infection Eur. Respir. J., October 1, 2001; 18(4): 692 - 704. [Abstract] [Full Text] [PDF]

				B. Dubois, C. Massacrier, and C. Caux Selective attraction of naive and memory B cells by dendritic cells J. Leukoc. Biol., October 1, 2001; 70(4): 633 - 641. [Abstract] [Full Text] [PDF]

				K. Takahashi, A. Kenji, T. Norihiro, K. Eisaku, O. Takashi, H. Kazuhiko, Y. Tadashi, and A. Tadaatsu Morphological Interactions of Interdigitating Dendritic Cells with B and T Cells in Human Mesenteric Lymph Nodes Am. J. Pathol., July 1, 2001; 159(1): 131 - 138. [Abstract] [Full Text] [PDF]

				A. George-Chandy, N. Mielcarek, I. Nordstrom, J. Holmgren, and K. Eriksson Vaccination with Bordetella pertussis-Pulsed Autologous or Heterologous Dendritic Cells Induces a Mucosal Antibody Response In Vivo and Protects against Infection Infect. Immun., June 1, 2001; 69(6): 4120 - 4124. [Abstract] [Full Text] [PDF]

				A. Kaser, S. Dunzendorfer, F. A. Offner, O. Ludwiczek, B. Enrich, R. O. Koch, W. W. Cruikshank, C. J. Wiedermann, and H. Tilg B Lymphocyte-Derived IL-16 Attracts Dendritic Cells and Th Cells J. Immunol., September 1, 2000; 165(5): 2474 - 2480. [Abstract] [Full Text] [PDF]

				D. Yang, Q. Chen, S. Stoll, X. Chen, O. M. Z. Howard, and J. J. Oppenheim Differential Regulation of Responsiveness to fMLP and C5a Upon Dendritic Cell Maturation: Correlation with Receptor Expression J. Immunol., September 1, 2000; 165(5): 2694 - 2702. [Abstract] [Full Text] [PDF]

				V. Moulin, F. Andris, K. Thielemans, C. Maliszewski, J. Urbain, and M. Moser B Lymphocytes Regulate Dendritic Cell (Dc) Function in Vivo: Increased Interleukin 12 Production by DCs from B Cell-Deficient Mice Results in T Helper Cell Type 1 Deviation J. Exp. Med., August 21, 2000; 192(4): 475 - 482. [Abstract] [Full Text] [PDF]

				J. M. Timmerman and R. Levy Linkage of Foreign Carrier Protein to a Self-Tumor Antigen Enhances the Immunogenicity of a Pulsed Dendritic Cell Vaccine J. Immunol., May 1, 2000; 164(9): 4797 - 4803. [Abstract] [Full Text] [PDF]

				B. Johansson, S. Ingvarsson, P. Bjorck, and C. A. K. Borrebaeck Human Interdigitating Dendritic Cells Induce Isotype Switching and IL-13-Dependent IgM Production in CD40-Activated Naive B Cells J. Immunol., February 15, 2000; 164(4): 1847 - 1854. [Abstract] [Full Text] [PDF]

				F.-P. Huang, N. Platt, M. Wykes, J. R. Major, T. J. Powell, C. D. Jenkins, and G. G. MacPherson A Discrete Subpopulation of Dendritic Cells Transports Apoptotic Intestinal Epithelial Cells to T Cell Areas of Mesenteric Lymph Nodes J. Exp. Med., February 7, 2000; 191(3): 435 - 444. [Abstract] [Full Text] [PDF]

				Y. Zhang, Y. Zhang, Y. Wang, M. Ogata, S.-i. Hashimoto, N. Onai, and K. Matsushima Development of dendritic cells in vitro from murine fetal liver-derived lineage phenotype-negative c-kit+ hematopoietic progenitor cells Blood, January 1, 2000; 95(1): 138 - 146. [Abstract] [Full Text] [PDF]

				D. Marshall, R. Sealy, M. Sangster, and C. Coleclough TH Cells Primed During Influenza Virus Infection Provide Help for Qualitatively Distinct Antibody Responses to Subsequent Immunization J. Immunol., November 1, 1999; 163(9): 4673 - 4682. [Abstract] [Full Text] [PDF]

				J. Skok, J. Poudrier, and D. Gray Dendritic Cell-Derived IL-12 Promotes B Cell Induction of Th2 Differentiation: A Feedback Regulation of Th1 Development J. Immunol., October 15, 1999; 163(8): 4284 - 4291. [Abstract] [Full Text] [PDF]

				C. Berney, S. Herren, C. A. Power, S. Gordon, L. Martinez-Pomares, and M. H. Kosco-Vilbois A Member of the Dendritic Cell Family That Enters B Cell Follicles and Stimulates Primary Antibody Responses Identified by a Mannose Receptor Fusion Protein J. Exp. Med., September 20, 1999; 190(6): 851 - 860. [Abstract] [Full Text] [PDF]