HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Graham, C. M. Articles by Thomas, D. B. Search for Related Content PubMed PubMed Citation Articles by Graham, C. M. Articles by Thomas, D. B.

Novel Diversity in Th1, Th2 Type Differentiation of Hemagglutinin-Specific T Cell Clones Elicited by Natural Influenza Virus Infection in Three Major Haplotypes (H-2^b,d,k)

National Institute for Medical Research, London, United Kingdom

We report novel diversity in the lymphokine (LK) secretion profile of hemagglutinin-specific, CD4⁺ T cell clones elicited by influenza virus infection in three major haplotypes: I-A^d- or I-E^d-restricted T cell clones obtained from individual BALB/c donors, and specific for three distinct antigenic peptides (p56–76, or p186–205 or p177–199), were uniformly Th1 type, releasing only IFN- on activation. In contrast, extensive diversity was evident for the C57BL/10 or CBA/Ca repertoire. Sibling T cell clones, established from the same C57BL/10 donor and expressing identical TCR ß-chains in their recognition of p186–205, released either (IFN- and IL-5) or (IFN- and IL-4 and IL-5) or (IL-4 and IL-5 and IL-10) following Ag-specific or nonspecific stimulation. Similarly, I-A^k-restricted T cell clones, specific for p120–139 secreted either (IFN- only) or (IFN- and IL-5) or (IFN- and IL-2 and IL-5) on activation. Despite such phenotypic diversity within the individual’s repertoire, all clones had been maintained under identical in vitro culture conditions. Moreover, sequence analyses of TCR ß gene usage indicated that in most instances clones from the same donor expressed identical (VDJ)ß rearrangements, indicative of a common progenitor cell. FACS analysis of cytoplasmic cytokine production confirmed that for the novel phenotype (IFN- and IL-5), both LKs were synthesized at the single cell level. Sibling families of T cell clones, established from a common donor following viral infection but differing in LK secretion, may offer a suitable model system for further studies of signal transduction mechanisms that discriminate between Th1- and Th2-specific responses to a well defined protective Ag.

This article has been cited by other articles:

				K. C. Fae, D. D. da Silva, S. E. Oshiro, A. C. Tanaka, P. M. A. Pomerantzeff, C. Douay, D. Charron, A. Toubert, M. W. Cunningham, J. Kalil, et al. Mimicry in Recognition of Cardiac Myosin Peptides by Heart-Intralesional T Cell Clones from Rheumatic Heart Disease J. Immunol., May 1, 2006; 176(9): 5662 - 5670. [Abstract] [Full Text] [PDF]

				P. A. Johnson, M. A. Conway, J. Daly, C. Nicolson, J. Robertson, and K. H. G. Mills Plasmid DNA encoding influenza virus haemagglutinin induces Th1 cells and protection against respiratory infection despite its limited ability to generate antibody responses J. Gen. Virol., July 1, 2000; 81(7): 1737 - 1745. [Abstract] [Full Text]