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*
Department of Immunology, Medical Clinic, University Hospital Eppendorf, Hamburg, Germany;
Institute of Cell Biology, University of Münster, Münster, Germany;
Heinrich Pette Institute, Hamburg, Germany; and
§
Institute for Experimental Medicine, University of Erlangen-Nürnberg, Erlangen, Germany
A. Hamann, F. Austrup, W. Tietz, and D. Jablonski-Westrich. Activation induces rapid and profound alterations in the trafficking of T cells. Submitted for publication.
Previous data suggested a role of endothelial selectins in skin homing of lymphocytes. In the current study, we have analyzed the expression and functional role of E-and P-selectin ligands on CD4+ T cells induced in vivo upon skin sensitization, using soluble selectin-Ig chimera and blocking Abs. Only low numbers of CD4+ cells expressing significant levels of E- or P-selectin ligands were present in s.c. lymph nodes of untreated mice (0.5–1.5% and 2–4%, respectively). Induction of a delayed-type hypersensitivity reaction increased the percentage of E-selectin-binding CD4+ cells in the draining lymph nodes up to 6 to 9% and that of P-selectin-binding cells up to 14%. The majority of E- and P-selectin-binding cells displayed an activated phenotype as judged by the increase in IL-2R, CD71, or cell size. The populations of E- and P-selectin-binding cells were largely overlapping; all E-selectin-binding cells also bound to P-selectin, whereas only a subfraction of P-selectin-binding cells reacted with E-selectin. Both E- and P-selectin-binding CD4+ cells, isolated by FACS, efficiently migrated into inflamed, but not normal skin, whereas P- or E-selectin ligand-negative CD4+ T cells did not. Abs against one of the two endothelial selectins partially inhibited the entry of isolated, ligand-positive cells, whereas a combination of Abs against both selectins almost completely abrogated skin homing. These data indicate that the expression of functional ligands for E- and for P-selectin is essential for homing of CD4+ T cells into the inflamed skin.
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R. L. Reinhardt, D. C. Bullard, C. T. Weaver, and M. K. Jenkins Preferential Accumulation of Antigen-specific Effector CD4 T Cells at an Antigen Injection Site Involves CD62E-dependent Migration but Not Local Proliferation J. Exp. Med., March 17, 2003; 197(6): 751 - 762. [Abstract] [Full Text] [PDF]
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J. L. Curtis, J. Sonstein, R. A. Craig, J. C. Todt, R. N. Knibbs, T. Polak, D. C. Bullard, and L. M. Stoolman3 Subset-Specific Reductions in Lung Lymphocyte Accumulation Following Intratracheal Antigen Challenge in Endothelial Selectin-Deficient Mice J. Immunol., September 1, 2002; 169(5): 2570 - 2579. [Abstract] [Full Text] [PDF]
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I. Erdmann, E. P. Scheidegger, F. K. Koch, L. Heinzerling, B. Odermatt, G. Burg, J. B. Lowe, and T. M. Kundig Fucosyltransferase VII-Deficient Mice with Defective E-, P-, and L-Selectin Ligands Show Impaired CD4+ and CD8+ T Cell Migration into the Skin, but Normal Extravasation into Visceral Organs J. Immunol., March 1, 2002; 168(5): 2139 - 2146. [Abstract] [Full Text] [PDF]
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A. C. Issekutz and T. B. Issekutz The Role of E-Selectin, P-Selectin, and Very Late Activation Antigen-4 in T Lymphocyte Migration to Dermal Inflammation J. Immunol., February 15, 2002; 168(4): 1934 - 1939. [Abstract] [Full Text] [PDF]
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T. Katakai, K. J. Mori, T. Masuda, and A. Shimizu Selective accumulation of type 1 effector cells expressing P-selectin ligand and/or {alpha}4{beta}7-integrin at the lesions of autoimmune gastritis Int. Immunol., February 1, 2002; 14(2): 167 - 175. [Abstract] [Full Text] [PDF]
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D. J. Campbell and E. C. Butcher Rapid Acquisition of Tissue-specific Homing Phenotypes by CD4+ T Cells Activated in Cutaneous or Mucosal Lymphoid Tissues J. Exp. Med., January 7, 2002; 195(1): 135 - 141. [Abstract] [Full Text] [PDF]
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D. A. Carlow, S. Y. Corbel, M. J. Williams, and H. J. Ziltener IL-2, -4, and -15 Differentially Regulate O-Glycan Branching and P-Selectin Ligand Formation in Activated CD8 T Cells J. Immunol., December 15, 2001; 167(12): 6841 - 6848. [Abstract] [Full Text] [PDF]
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Y. Reiss, A. E. Proudfoot, C. A. Power, J. J. Campbell, and E. C. Butcher CC Chemokine Receptor (CCR)4 and the CCR10 Ligand Cutaneous T Cell-attracting Chemokine (CTACK) in Lymphocyte Trafficking to Inflamed Skin J. Exp. Med., November 19, 2001; 194(10): 1541 - 1547. [Abstract] [Full Text] [PDF]
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Y.-C. Lim, H. Xie, C. E. Come, S. I. Alexander, M. J. Grusby, A. H. Lichtman, and F. W. Luscinskas IL-12, STAT4-Dependent Up-Regulation of CD4+ T Cell Core 2 {beta}-1,6-n-Acetylglucosaminyltransferase, an Enzyme Essential for Biosynthesis of P-Selectin Ligands J. Immunol., October 15, 2001; 167(8): 4476 - 4484. [Abstract] [Full Text] [PDF]
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K. Tanigawa, N. Takeshita, R. A. Craig, K. Phillips, R. N. Knibbs, A. E. Chang, and L. M. Stoolman Tumor-Specific Responses in Lymph Nodes Draining Murine Sarcomas Are Concentrated in Cells Expressing P-Selectin Binding Sites J. Immunol., September 15, 2001; 167(6): 3089 - 3098. [Abstract] [Full Text] [PDF]
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G. Smithson, C. E. Rogers, P. L. Smith, E. P. Scheidegger, B. Petryniak, J. T. Myers, D. S. L. Kim, J. W. Homeister, and J. B. Lowe Fuc-Tvii Is Required for T Helper 1 and T Cytotoxic 1 Lymphocyte Selectin Ligand Expression and Recruitment in Inflammation, and Together with Fuc-Tiv Regulates Naive T Cell Trafficking to Lymph Nodes J. Exp. Med., September 3, 2001; 194(5): 601 - 614. [Abstract] [Full Text] [PDF]
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K. Singbartl, J. Thatte, M. L. Smith, K. Wethmar, K. Day, and K. Ley A CD2-Green Fluorescence Protein-Transgenic Mouse Reveals Very Late Antigen-4-Dependent CD8+ Lymphocyte Rolling in Inflamed Venules J. Immunol., June 15, 2001; 166(12): 7520 - 7526. [Abstract] [Full Text] [PDF]
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K. Hong, E. L. Berg, and R. O. Ehrhardt Persistence of Pathogenic CD4+ Th1-Like Cells In Vivo in the Absence of IL-12 but in the Presence of Autoantigen J. Immunol., April 1, 2001; 166(7): 4765 - 4772. [Abstract] [Full Text] [PDF]
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T. M. Engeman, A. V. Gorbachev, R. P. Gladue, P. S. Heeger, and R. L. Fairchild Inhibition of Functional T Cell Priming and Contact Hypersensitivity Responses by Treatment with Anti-Secondary Lymphoid Chemokine Antibody During Hapten Sensitization J. Immunol., May 15, 2000; 164(10): 5207 - 5214. [Abstract] [Full Text] [PDF]
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C. Bartholdy, O. Marker, and A. R. Thomsen Migration of activated CD8+ T lymphocytes to sites of viral infection does not require endothelial selectins Blood, February 15, 2000; 95(4): 1362 - 1369. [Abstract] [Full Text] [PDF]
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B. A. Zabel, W. W. Agace, J. J. Campbell, H. M. Heath, D. Parent, A. I. Roberts, E. C. Ebert, N. Kassam, S. Qin, M. Zovko, et al. Human G Protein-Coupled Receptor Gpr-9-6/Cc Chemokine Receptor 9 Is Selectively Expressed on Intestinal Homing T Lymphocytes, Mucosal Lymphocytes, and Thymocytes and Is Required for Thymus-Expressed Chemokine-Mediated Chemotaxis J. Exp. Med., November 1, 1999; 190(9): 1241 - 1256. [Abstract] [Full Text] [PDF]
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A. Chu, K. Hong, E. L. Berg, and R. O. Ehrhardt Tissue Specificity of E- and P-Selectin Ligands in Th1-Mediated Chronic Inflammation J. Immunol., November 1, 1999; 163(9): 5086 - 5093. [Abstract] [Full Text] [PDF]
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J. M. Blander, I. Visintin, C. A. Janeway Jr., and R. Medzhitov {alpha}(1,3)-Fucosyltransferase VII and {alpha}(2,3)-Sialyltransferase IV Are Up-Regulated in Activated CD4 T Cells and Maintained After Their Differentiation into Th1 and Migration into Inflammatory Sites J. Immunol., October 1, 1999; 163(7): 3746 - 3752. [Abstract] [Full Text] [PDF]
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S. Trembleau, G. Penna, S. Gregori, H. D. Chapman, D. V. Serreze, J. Magram, and L. Adorini Pancreas-Infiltrating Th1 Cells and Diabetes Develop in IL-12-Deficient Nonobese Diabetic Mice J. Immunol., September 1, 1999; 163(5): 2960 - 2968. [Abstract] [Full Text] [PDF]
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H. Xie, Y.-C. Lim, F. W. Luscinskas, and A. H. Lichtman Acquisition of Selectin Binding and Peripheral Homing Properties by CD4+ and CD8+ T Cells J. Exp. Med., June 7, 1999; 189(11): 1765 - 1776. [Abstract] [Full Text] [PDF]
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