| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
*
Vascular Research Division, Department of Pathology, Brigham and Women’s Hospital,
Cardiovascular Research Center, Massachusetts General Hospital-East, Harvard Medical School, Boston, MA
E-selectin, a selectin expressed on activated vascular endothelium, supports rolling and stable adhesion of leukocytes at sites of inflammation. Previously, we have reported that leukocyte adhesion to cultured endothelial cells induces association of the cytoplasmic domain of E-selectin with cytoskeletal elements, suggesting that outside-in signaling may occur during E-selectin-mediated adhesion. To investigate this potential signaling function of E-selectin, HUVEC activated with recombinant human IL-1ß (10 U/ml, 4 h) were labeled with [32P]orthophosphate, and E-selectin was immunoprecipitated using mAb H18/7. Autoradiography revealed constitutive phosphorylation of E-selectin in these cells and time-dependent dephosphorylation following adhesion of HL-60 cells. Cross-linking of cell surface E-selectin using H18/7 and a polyclonal secondary Ab induced E-selectin dephosphorylation, as did adhesion of beads coated with recombinant P-selectin glycoprotein ligand-1 (PSGL-1), an E-selectin ligand. Using adenoviral vector-mediated transfection in HUVEC of a tail-less E-selectin and phosphoamino acid analysis, we documented phosphorylation occurring exclusively within the cytoplasmic domain and involving serine residues. Additional experiments using a series of cytoplasmic domain mutants of E-selectin expressed in COS-7 cells localized the regions that were constitutively phosphorylated. Preincubation with okadaic acid and sodium vanadate abrogated adhesion-induced dephosphorylation of E-selectin. Thus, E-selectin, which is constitutively phosphorylated in cytokine-activated human endothelial cells, undergoes an enzymatically regulated dephosphorylation following leukocyte adhesion. This process appears to be triggered by multivalent ligand binding and/or cross-linking of cell surface E-selectin. Ligand-dependent regulation of the phosphorylation of E-selectin’s cytoplasmic domain provides additional evidence for a transmembrane signaling function of this molecule during leukocyte-endothelial interactions.
This article has been cited by other articles:
|
|
 |
|
  H. Setiadi and R. P. McEver Clustering endothelial E-selectin in clathrin-coated pits and lipid rafts enhances leukocyte adhesion under flow Blood, February 15, 2008; 111(4): 1989 - 1998. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Osaka, S. Hagita, M. Haraguchi, M. Kajimura, M. Suematsu, and M. Yoshida Real-time imaging of mechanically injured femoral artery in mice reveals a biphasic pattern of leukocyte accumulation Am J Physiol Heart Circ Physiol, April 1, 2007; 292(4): H1876 - H1882. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Nishiwaki, M. Yoshida, H. Iwaguro, H. Masuda, N. Nitta, T. Asahara, and M. Isobe Endothelial E-Selectin Potentiates Neovascularization via Endothelial Progenitor Cell-Dependent and -Independent Mechanisms Arterioscler. Thromb. Vasc. Biol., March 1, 2007; 27(3): 512 - 518. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Galkina and K. Ley Leukocyte Recruitment and Vascular Injury in Diabetic Nephropathy J. Am. Soc. Nephrol., February 1, 2006; 17(2): 368 - 377. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Yu, K. S. Moulton, M. K. Khan, S. Vineberg, E. Boye, V. M. Davis, P. E. O'Donnell, J. Bischoff, and D. S. Milstone E-selectin is required for the antiangiogenic activity of endostatin PNAS, May 25, 2004; 101(21): 8005 - 8010. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J.-M. Kiely, Y. Hu, G. Garcia-Cardena, and M. A. Gimbrone Jr. Lipid Raft Localization of Cell Surface E-Selectin Is Required for Ligation-Induced Activation of Phospholipase C{gamma} J. Immunol., September 15, 2003; 171(6): 3216 - 3224. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Yoshida, Y. Takano, T. Sasaoka, T. Izumi, and A. Kimura E-Selectin Polymorphism Associated With Myocardial Infarction Causes Enhanced Leukocyte-Endothelial Interactions Under Flow Conditions Arterioscler. Thromb. Vasc. Biol., May 1, 2003; 23(5): 783 - 788. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. S. Kluger, S. L. Shiao, A. L. M. Bothwell, and J. S. Pober Cutting Edge: Internalization of Transduced E-Selectin by Cultured Human Endothelial Cells: Comparison of Dermal Microvascular and Umbilical Vein Cells and Identification of a Phosphoserine-Type Di-leucine Motif J. Immunol., March 1, 2002; 168(5): 2091 - 2095. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Hu, B. Szente, J.-M. Kiely, and M. A. Gimbrone Jr. Molecular Events in Transmembrane Signaling via E-selectin. SHP2 ASSOCIATION, ADAPTOR PROTEIN COMPLEX FORMATION AND ERK1/2 ACTIVATION J. Biol. Chem., December 14, 2001; 276(51): 48549 - 48553. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Yoshida, T. Sawada, H. Ishii, R. E. Gerszten, A. Rosenzweig, M. A. Gimbrone Jr, Y. Yasukochi, and F. Numano HMG-CoA Reductase Inhibitor Modulates Monocyte-Endothelial Cell Interaction Under Physiological Flow Conditions In Vitro : Involvement of Rho GTPase-Dependent Mechanism Arterioscler. Thromb. Vasc. Biol., July 1, 2001; 21(7): 1165 - 1171. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. E. ERIKSSON, X. XIE, J. WERR, P. THOREN, and L. LINDBOM Direct viewing of atherosclerosis in vivo: plaque invasion by leukocytes is initiated by the endothelial selectins FASEB J, May 1, 2001; 15(7): 1149 - 1157. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Ishii, M. Yoshida, A. Rosenzweig, M. A. Gimbrone Jr, Y. Yasukochi, and F. Numano Adenoviral transduction of human E-selectin into isolated, perfused, rat aortic segments: an ex vivo model for studying leukocyte-endothelial interactions J. Leukoc. Biol., November 1, 2000; 68(5): 687 - 692. [Abstract] [Full Text]
|
|
|
|
|
|
Y. Hu, J.-M. Kiely, B. E. Szente, A. Rosenzweig, and M. A. Gimbrone Jr. E-Selectin-Dependent Signaling Via the Mitogen-Activated Protein Kinase Pathway in Vascular Endothelial Cells J. Immunol., August 15, 2000; 165(4): 2142 - 2148. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. R. Allport, W. A. Muller, and F. W. Luscinskas Monocytes Induce Reversible Focal Changes in Vascular Endothelial Cadherin Complex during Transendothelial Migration Under Flow J. Cell Biol., January 10, 2000; 148(1): 203 - 216. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
