HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Chen, W. Articles by Cheever, M. A. Search for Related Content PubMed PubMed Citation Articles by Chen, W. Articles by Cheever, M. A.

T Cells Specific for a Polymorphic Segment of CD45 Induce Graft-Versus-Host Disease with Predominant Pulmonary Vasculitis¹

Wei Chen^2,*, Gurkamal S. Chatta^3,*, William D. Rubin^*, Joan G. Clark^§, Robert C. Hackman^,§, David K. Madtes^§, Denny H. Ligitt, Yoichiro Kusunoki^§, Paul J. Martin^§ and Martin A. Cheever^4,*

^* Division of Oncology, Departments of Medicine, Comparative Medicine, and Pathology, University of Washington, Seattle, WA 98195; and ^§ Fred Hutchinson Cancer Research Center, Seattle, WA 98104

To study the character of graft-vs-host disease (GVHD) induced by T cells specific for hemopoietic cells, T cells specific for a polymorphic segment of CD45 were transferred into CD45 congenic mice. C57BL/6 mice that express the CD45b allele were immunized with a 13 mer peptide representing the polymorphic segment (p_257–268) of CD45a protein. Conversely, C57BL/6 mice congenic for CD45a were immunized with the CD45b peptide. CD4⁺ T cells specific for allelic CD45 peptides were elicited. Importantly, T cells specific for CD45 peptides proliferated specifically and vigorously in response to spleen cells expressing the appropriate polymorphic CD45 protein. T cells specific for CD45 induced a substantial graft-vs-host response (GVHR) with predominant early pulmonary vasculitis and later more widespread interstitial mononuclear cell infiltration and alveolitis. No GVHR was induced in bone marrow chimeras expressing only donor hemopoietic cells. Thus, donor T cell recognition of host hemopoietic cells is sufficient to elicit GVHR, but the classical skin, liver, and gut manifestations of GVHD were not observed. The CD45-specific T cells used secreted Th1 cytokines, but without detectable soluble IL-2. Studies using CD45-specific T cells with different effector functions might allow further dissection of donor cell requirements for GVHD syndromes.

This article has been cited by other articles:

				K.-H. Kim, K. Burkhart, P. Chen, C. W. Frevert, J. Randolph-Habecker, R. C. Hackman, P. D. Soloway, and D. K. Madtes Tissue Inhibitor of Metalloproteinase-1 Deficiency Amplifies Acute Lung Injury in Bleomycin-Exposed Mice Am. J. Respir. Cell Mol. Biol., September 1, 2005; 33(3): 271 - 279. [Abstract] [Full Text] [PDF]

				J. G. Clark, J. B. Mandac-Dy, A. E. Dixon, D. K. Madtes, K. M. Burkhart, J. M. Harlan, and D. C. Bullard Trafficking of Th1 Cells to Lung: A Role for Selectins and a P-Selectin Glycoprotein-1-Independent Ligand Am. J. Respir. Cell Mol. Biol., February 1, 2004; 30(2): 220 - 227. [Abstract] [Full Text] [PDF]

				J. Thatte, V. Dabak, M. B. Williams, T. J. Braciale, and K. Ley LFA-1 is required for retention of effector CD8 T cells in mouse lungs Blood, June 15, 2003; 101(12): 4916 - 4922. [Abstract] [Full Text] [PDF]

				R. van Os, T. M. Sheridan, S. Robinson, D. Drukteinis, J. L.M. Ferrara, and P. M. Mauch Immunogenicity of Ly5 (CD45)-Antigens Hampers Long-Term Engraftment Following Minimal Conditioning in a Murine Bone Marrow Transplantation Model Stem Cells, January 1, 2001; 19(1): 80 - 87. [Abstract] [Full Text]

				A. E. Dixon, J. B. Mandac, P. J. Martin, R. C. Hackman, D. K. Madtes, and J. G. Clark Adherence of adoptively transferred alloreactive Th1 cells in lung: partial dependence on LFA-1 and ICAM-1 Am J Physiol Lung Cell Mol Physiol, September 1, 2000; 279(3): L583 - L591. [Abstract] [Full Text] [PDF]

				A. E. Dixon, J. B. Mandac, D. K. Madtes, P. J. Martin, and J. G. Clark Chemokine expression in Th1 cell-induced lung injury: prominence of IFN-gamma -inducible chemokines Am J Physiol Lung Cell Mol Physiol, September 1, 2000; 279(3): L592 - L599. [Abstract] [Full Text] [PDF]

				A. E. Dixon, J. B. Mandac, P. J. Martin, D. K. Madtes, R. C. Hackman, and J. G. Clark Alloreactive Th1 Cells Localize in Lung and Induce Acute Lung Injury Chest, July 1, 1999; 116(2007): 36S - 37S. [Full Text]

				P. A. Eden, G. J. Christianson, P. Fontaine, P. J. Wettstein, C. Perreault, and D. C. Roopenian Biochemical and Immunogenetic Analysis of an Immunodominant Peptide (B6dom1) Encoded by the Classical H7 Minor Histocompatibility Locus J. Immunol., April 15, 1999; 162(8): 4502 - 4510. [Abstract] [Full Text] [PDF]

				J. G. Clark, D. K. Madtes, R. C. Hackman, W. Chen, M. A. Cheever, and P. J. Martin Lung Injury Induced by Alloreactive Th1 Cells Is Characterized by Host-Derived Mononuclear Cell Inflammation and Activation of Alveolar Macrophages J. Immunol., August 15, 1998; 161(4): 1913 - 1920. [Abstract] [Full Text] [PDF]