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Departments of
*
Microbiology and Immunology and
Pathology and Laboratory Medicine, University of California Los Angeles School of Medicine, Los Angeles, CA 90095; and
Emory Vaccine Center & Department of Microbiology and Immunology, Emory University School of Medicine, Atlanta, GA 30322
Glycosylation changes on surface molecules of T cells affect cell trafficking and function and may be useful in discriminating between naive, effector, and memory T cells. To analyze oligosaccharide structures on T cells activated in vivo, we examined alterations in sialic acid residues on T cells following infection of mice with lymphocytic choriomeningitis (LCMV), vaccinia virus, and vesicular stomatitis virus. We found that the majority of CD8 T cells from mice acutely infected with these viruses showed increased binding to peanut agglutinin (PNA). All of the PNAhighCD8 T cells from infected mice were CD44high, indicating that glycosylation changes were occurring on activated T cells. There was also an increase in the PNAhighCD4 T cell population in virally infected mice. Increased PNA binding to activated CD8 T cells correlated with higher endogenous neuraminidase levels in these cells. This higher neuraminidase activity most likely contributed to the PNAhigh phenotype by cleaving sialic acid residues off the core-1 O-glycans or glycoproteins destined for the cell surface. A PNAhighCD8 T cell population persisted in immune mice that had cleared the LCMV infection. When spleen cells from immune mice were sorted into PNAhigh and PNAlow populations, >95% of the LCMV-specific memory CD8 T cells segregated with the PNAhigh population. This shows that virus-specific memory CD8 T cells remain hyposialylated and can be distinguished from naive CD8 T cells based on PNA binding. Thus, PNA can be used as a marker for Ag-experienced T cells.
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M. Kaufmann, C. Blaser, S. Takashima, R. Schwartz-Albiez, S. Tsuji, and H. Pircher Identification of an {alpha}2,6-sialyltransferase induced early after lymphocyte activation Int. Immunol., May 1, 1999; 11(5): 731 - 738. [Abstract] [Full Text] [PDF]
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